Antibiofilm Effect of Trans-Cinnamaldehyde on Uropathogenic Escherichia coli

Amalaradjou, Mary Anne Roshni; Narayanan, Amoolya; Baskaran, Sangeetha Ananda; Venkitanarayanan, Kumar

doi:10.1016/j.juro.2010.03.006

Cited by 75 publications

(62 citation statements)

References 25 publications

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“…Moreover, TC used as an antimicrobial constituent in a catheter lock solution effectively inactivated already-formed E. coli biofilm. This observation confirms the previous report by Amalaradjou et al (14), who showed that TC was effective against UPEC biofilm on polystyrene or latex, and that the expression of E. coli genes encoding attachment and invasion of bladder cells was significantly decreased by TC (15). In our study, p-CA had high activity in UPEC biofilm prevention.…”

Section: Discussionsupporting

confidence: 93%

“…Biofilm formation on the inner or outer surface of the urinary catheter plays a main role in the pathogenesis of CAUTI and is a major reservoir of resistant pathogens (6). Different methods of CAUTI prevention have been evaluated, including the use of catheters coated with various materials targeted against the pathogenesis of biofilms (19,20) or bladder and catheter irrigation with antimicrobial solutions (1,14,21). Treatment with prophylactic antibiotics is not recommended because it is not always effective in preventing CAUTI and the effect is only temporary.…”

Section: Discussionmentioning

confidence: 99%

“…The chemical structures of TC, p-CA, and FA are shown in Figure 1. Although the antibiofilm effect of TC on UPEC was reported earlier (14,15), the efficiency of p-CA and FA against UPEC biofilm formed on urinary catheters has not been determined.…”

Section: Introductionmentioning

confidence: 99%

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Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

Kot

Wicha²,

Piechota³

et al. 2015

Turk J Med Sci

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Background/aim: Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters.Materials and methods: Biofilm prevention was tested using catheter fragments inoculated with E. coli and treated with transcinnamaldehyde, p-coumaric, and ferulic acids (0%, 0.1%, 0.25%, and 0.5%) for 0, 1, 3, and 5 days. Inactivation of E. coli biofilm with the same agents at concentrations of 0%, 1%, 1.25%, or 1.5% used for 0, 1, 3, or 5 days was also evaluated.Results: All used concentrations of trans-cinnamaldehyde prevented and effectively inactivated E. coli biofilm formed on urinary catheter fragments. p-Coumaric (0.25% and 0.5%) and ferulic acids (0.5%) had preventive action on E. coli biofilm formation in urinary catheter fragments. The number of uropathogenic E. coli cells in biofilm formed in the lumen of a urinary catheter was significantly reduced in the presence of p-coumaric and ferulic acids, but complete inactivation of the biofilm formed was not observed, as opposed to the use of trans-cinnamaldehyde. Conclusion:The obtained results indicate that phytochemicals may be an important source of antibiofilm agents that have preventive action on E. coli biofilm formation on urinary catheters.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

Kot

Wicha²,

Piechota³

et al. 2015

Turk J Med Sci

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“…Previously, our laboratory observed that TC was effective at reducing S. Enteritidis in chicken cecal contents in vitro and in various internal organs in broilers (38). In addition, TC was found to inhibit biofilm formation by Cronobacter sakazakii (39) and uropathogenic Escherichia coli (40), by downregulating critical genes involved in biofilm synthesis.…”

mentioning

confidence: 96%

In-Feed Supplementation of trans -Cinnamaldehyde Reduces Layer-Chicken Egg-Borne Transmission of Salmonella enterica Serovar Enteritidis

Upadhyaya

Upadhyay

Johny

et al. 2015

Appl Environ Microbiol

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c Salmonella enterica serovar Enteritidis is a major foodborne pathogen in the United States, causing gastroenteritis in humans, primarily through consumption of contaminated eggs. Chickens are the reservoir host of S. Enteritidis. In layer hens, S. Enteritidis colonizes the intestine and migrates to various organs, including the oviduct, leading to egg contamination. This study investigated the efficacy of in-feed supplementation with trans-cinnamaldehyde (TC), a generally recognized as safe (GRAS) plant compound obtained from cinnamon, in reducing S. Enteritidis cecal colonization and systemic spread in layers. Additionally, the effect of TC on S. Enteritidis virulence factors critical for macrophage survival and oviduct colonization was investigated in vitro. The consumer acceptability of eggs was also determined by a triangle test. Supplementation of TC in feed for 66 days at 1 or 1.5% (vol/wt) for 40-or 25-week-old layer chickens decreased the amounts of S. Enteritidis on eggshell and in yolk (P < 0.001). Additionally, S. Enteritidis persistence in the cecum, liver, and oviduct in TC-supplemented birds was decreased compared to that in controls (P < 0.001). No significant differences in feed intake, body weight, or egg production in birds or in consumer acceptability of eggs were observed (P > 0.05). In vitro cell culture assays revealed that TC reduced S. Enteritidis adhesion to and invasion of primary chicken oviduct epithelial cells and reduced S. Enteritidis survival in chicken macrophages (P < 0.001). Follow-up gene expression analysis using real-time quantitative PCR (qPCR) showed that TC downregulated the expression of S. Enteritidis virulence genes critical for chicken oviduct colonization (P < 0.001). The results suggest that TC may potentially be used as a feed additive to reduce egg-borne transmission of S. Enteritidis. Salmonella enterica serovar Enteritidis is one of the major foodborne pathogens in the United States responsible for causing enteric illnesses in humans (1). Eggs are the primary source of S. Enteritidis infection of humans (1, 2). Approximately 90 billion eggs are produced and 67.5 billion shell eggs consumed annually in the United States (3). Thus, the microbiological safety of eggs is a major concern to the government, the poultry industry, and consumers due to the potential impacts on public health and the economy. Chickens act as asymptomatic carriers of S. Enteritidis, resulting in its environmental dissemination and potential infection of humans. Humans contract S. Enteritidis infection via consumption of contaminated, raw, or undercooked eggs, and several epidemiological studies have confirmed this association between human salmonellosis and egg consumption (4, 5).Despite the implementation of various pre-and postharvest control measures, S. Enteritidis remains a major cause of eggborne disease outbreaks in the United States (1). Recently, the U.S. Centers for Disease Control and Prevention (CDC) reported that the incidence of foodborne salmonellosis did not decrease signi...

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“…Although, cinnamon or cinnamon oil has been used for ages in the treatment of UTIs, no scientific study was undertaken to investigate its antimicrobial efficacy against uropathogens. Amalaradjou et al (2010) were the first to demonstrate the ability of trans-cinnamaldehyde to inactivate and inhibit UPEC biofilm formation on urinary www.intechopen.com catheters. A follow up study conducted by the same group indicated that trans-cinnamaldehyde inhibited the adhesion and invasion of uroepithelial cells by UPEC by downregulating major virulence genes in the pathogen.…”

Section: Trans-cinnamaldehydementioning

confidence: 99%

Natural Approaches for Controlling Urinary Tract Infections

Amalaradjou¹,

Venkitanarayanan²

2011

Urinary Tract Infections

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Antibiofilm Effect of Trans-Cinnamaldehyde on Uropathogenic Escherichia coli

Abstract: Results suggest that trans-cinnamaldehyde may be applied as a catheter surface coating or as an ingredient in catheter lock solution to prevent urinary tract infection in humans.

Cited by 75 publications

References 25 publications

Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

In-Feed Supplementation of trans -Cinnamaldehyde Reduces Layer-Chicken Egg-Borne Transmission of Salmonella enterica Serovar Enteritidis

Natural Approaches for Controlling Urinary Tract Infections

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