Antibiofilm and immunological properties of lectin purified from shrimp Penaeus semisulcatus

Elumalai, Preetham; Lakshmi, Sreeja; Wongpanya, Ratree; Vaseeharan, Baskaralingam; Arockiaraj, Jesu; Olsen, Rolf Erik

doi:10.1016/j.fsi.2020.07.053

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…Protein purification is often tasking, time-consuming and sometimes result in the generation of unexpected results, however, attempts are being made at simplifying specific purification process taking consideration of yield, purity and biological activity of the targeted protein [51] . In recent times, diverse chromatographic techniques are being used in the purification of lectins, exploiting the size, charge, or ligand affinity of the lectin [52] , [53] , [54] , [55] . MoL was purified to homogeneity via a simple and efficient two-step purification process involving ammonium sulfate precipitation and size exclusion chromatography ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Nubi

Adewole²,

Agunbiade

et al. 2021

Biotechnology Reports

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Highlights Purification of Moringa oleifera seed lectin (MoL) was achieved by ammonium sulfate precipitation and size exclusion chromatography MoL hemagglutinating activity was thermostable up to 90°C, within pH 2-4 and moderately inhibited by sucrose and fructose Traces of Ca2+, Mg2+, and Na+ was detected in MoL, and modification of arginine and tryptophan residues affected the hemagglutinating activity Hemolytic (membrane-perturbing) activity of MoL was potentiated in mildly acidic pH, and dependent on concentration, temperature, pH, and time of incubation.

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Section: Resultsmentioning

confidence: 99%

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Nubi

Adewole²,

Agunbiade

et al. 2021

Biotechnology Reports

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“…The supernatant was collected and transferred to 30 mL acetonitrile-saturated n-hexane, followed by agitation for 10 min to remove the fat. Finally, the organic solvents were evaporated by heating the mixture in a water bath at 80 °C, and the dried residue was dissolved in 50 mL of binding buffer and diluted 10 times for use [ 25 , 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

et al. 2022

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In this study, we developed a fluorescent aptasensor based on Fe3O4/Au/g-C3N4 and a FAM-labeled aptamer (FAM-SMZ1S) against sulfamethazine (SMZ) for the specific and sensitive detection of SMZ in food matrix. The FAM-SMZ1S was adsorbed by the Fe3O4/Au/g-C3N4 via π–π stacking and electrostatic adsorption, serving as a basis for the ultrasensitive detection of SMZ. Molecular dynamics was used to explain the reasons why SMZ1S and SMZ were combined. This aptasensor presented sensitive recognition performance, with a limit of detection of 0.16 ng/mL and a linear range of 1–100 ng/mL. The recovery rate ranged from 91.6% to 106.8%, and the coefficient of variation (CV) ranged from 2.8% to 13.4%. In addition, we tested the aptasensor for the monitoring of SMZ in various matrix samples, and the results were well-correlated (R2 ≥ 0.9153) with those obtained for HPLC detection. According to these results, the aptasensor was sensitive and accurate, representing a potentially useful tool for the detection of SMZ in food matrix.

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“…Lectins are produced by microorganisms, plants, and animals [37,38]. However, the largest number of known lectins are from the plant kingdom.…”

Section: Generalitiesmentioning

confidence: 99%

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

Lima

Patriota

Albuquerque

et al. 2020

AIR

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Depression and anxiety disorder are the most common mental disorders worldwide and their treatments are combinations of pharmacological and psychotherapeutic approaches. Depression treatment depends largely on a pharmacotherapy that improves the transmission of monoamines in the brain. However, the drugs available have adverse reactions and do not contemplate positively all patients, which stimulates scientific research that seeks new molecules, including from natural sources. Lectins are proteins capable of binding reversibly and non-covalently to specific sugars. For example, it has been reported the antimicrobial, antitumor, antiparasitic, anti-inflammatory and antinociceptive activities of lectins. The ability of some lectins in modulating nociception and inflammation stimulated studies on the possible effects on processes that share some pathways and molecular agents, like depression and anxiety. Lectins isolated from plants showed antidepressant effects, which were demonstrated to be linked to activation of serotonergic, adrenergic, and dopaminergic systems as well as to inhibition of the glutamatergic system and L-arginine–NO–cGMP pathway. In view of their immunomodulatory properties, it is also suggested that lectins can ameliorate the inflammatory framework associated with depression. Anxiolytic effects were also reported and associated with modulation of GABAergic mechanisms, serotonergic system, and NO pathway. It should be taken in account that some lectins induced depressive-like behavior, associated with an neuroinflammatory action, as well anxiogenic action. Thus, it is important to use combinations of batteries for testing anxiety, depression, despair, and anhedonia behaviors in the studies with lectins. The mechanisms by which lectins exactly modulate depression or anxiety frameworks are still unclear but important windows had already been open by researchers and preclinical studies with lectins have indicated these proteins as candidates for alternative or complementary agents in therapies of depression and anxiety disorder.

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Antibiofilm and immunological properties of lectin purified from shrimp Penaeus semisulcatus

Cited by 12 publications

References 25 publications

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

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