Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

Drago, Lorenzo; Bortolin, Monica; Vecchi, Elena De; Agrappi, Serse; Weinstein, R; Mattina, Roberto; Francetti, Luca

doi:10.1080/1120009x.2016.1158489

Cited by 32 publications

(32 citation statements)

References 39 publications

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“…The quantitative results assessed by both CLSM and qPCR resulted in a higher biomass volume and higher numbers of total bacteria on moderate‐roughness surface implants. These results are in agreement with those reported by Bevilacqua, Milan, Del Lupo, Maglione and Dolzani () or Drago et al (), in terms of biomass, and by Burgers et al (), in terms of qPCR, thus supporting the hypothesis that moderate‐roughness surfaces accumulate more bacteria and larger biofilms. Furthermore, these investigations demonstrated significantly higher concentrations of F. nucleatum and A. actinomycetemcomitans in moderate‐roughness surfaces when compared to minimal‐roughness surface implants .…”

Section: Discussionsupporting

confidence: 93%

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

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Objectives:To compare biofilm formation on whole dental titanium implants with different surface micro-topography. Methods:A multispecies in vitro biofilm model consisting of initial (Streptococcus oralis and Actinomyces naeslundii), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late colonizers (Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans) was grown for 96 hr on sterile titanium dental implants with either minimal (S a : 0.5-1.0 mm) or moderate-roughness titanium surfaces (S a : 1.1-2.0 mm).The resulting biofilms were studied with Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscope. Concentrations (colony-forming units per mL [CFU/ml]) of each bacterium were measured by quantitative Polymerase Chain Reaction (qPCR) and compared by Student t tests.Results: A biofilm, located mainly at the peak and lateral areas of the implant threads, was observed on both implant surfaces, with a greater biomass and a greater live/dead ratio in moderate-compared to minimal-roughness surface implants. Statistically significant higher values of total bacteria (mean difference = 2.61 × 10 7 CFU/ml; 95% confidence interval -CI [1.91 × 10 6 ; 5.02 × 10 7 ]; p = 0.036), F. Nucleatum (mean difference = 4.43 × 10 6 CFU/ml; 95% CI [1.06 × 10 6 ; 7.80 × 10 6 ]; p = 0.013) andA. actinomycetemcomitans (mean difference = 2.55 × 10 7 CFU/ml; 95% CI [1.07 × 10 7 ; 4.04 × 10 7 ]; p = 0.002), were found in the moderate-compared to minimal-roughness surface dental implants.Conclusions: Implants with moderate-roughness surfaces accumulated more bacterial biomass and significant higher number of pathogenic bacteria (F. nucleatum and A. actinomycetemcomitans), when compared to implants with minimal-roughness surfaces, within a similar biofilm structure.

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Section: Discussionsupporting

confidence: 93%

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

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“…Peri-implant biofilm was mimicked by cell adhesion to titanium surfaces, using a previously described methodology [23,38]. In spite of being an in vitro model, the comparison between mono-species of C. albicans and co-culture biofilms of C. albicans and C. glabrata allowed the authors to evaluate potential of chemical agents involved in the control of peri-implantitis biofilms.…”

Section: Discussionmentioning

confidence: 99%

“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Martorano-Fernandes

Cavalcanti

Almeida

2020

BMC Complement Med Ther

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Background: Peri-implant inflammation resulting from the presence of Candida biofilms may compromise the longevity of implant-supported dentures. This study evaluated the inhibitory effect of Brazilian red propolis on mono-species biofilms of C. albicans (ATCC 90028) and co-culture biofilms of C. albicans (ATCC 90028) and C. glabrata (ATCC 2001), developed on titanium surfaces. Methods: Titanium specimens were pre-conditioned with artificial saliva and submitted to biofilm formation (1 × 10 6 CFU/mL). After 24 h (under microaerophilic conditions at 37°C) biofilms were submitted to treatment for 10 min, according to the groups: sterile saline solution (growth control), 0.12% chlorhexidine and 3% red propolis extract. Treatments were performed every 24 h for 3 days and analyses were conducted 96 h after initial adhesion. After that, the metabolic activity (MTT assay) (n = 12/group), cell viability (CFU counts) (n = 12/group) and surface roughness (optical profilometry) (n = 6/group) were evaluated. Data from viability and metabolic activity assays were evaluated by ANOVA and Tukey tests. Surface roughness analysis was determined by Kruskal Wallis e Mann Whitney tests. Results: Regarding the mono-species biofilm, the cell viability and the metabolic activity showed that both chlorhexidine and red propolis had inhibitory effects and reduced the metabolism of biofilms, differing statistically from the growth control (p < 0.05). With regards the co-culture biofilms, chlorhexidine had the highest inhibitory effect (p < 0.05). The metabolic activity was reduced by the exposure to chlorhexidine and to red propolis, different from the growth control group (p < 0.05). The surface roughness (Sa parameter) within the mono-species and the co-culture biofilms statistically differed among groups (p < 0.05). Conclusions: Brazilian red propolis demonstrated potential antifungal activity against Candida biofilms, suggesting it is a feasible alternative for the treatment of peri-implantitis.

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“…Results showed a significantly lower total volume of the biomass on laser-modified surfaces, while no significant changes in live/dead bacteria were noticed between materials [224].…”

Section: Bacterial Adhesionmentioning

confidence: 87%

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

Baena

Rizzo

Manzo

et al. 2017

Journal of Nanomaterials

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Nanotechnology enables the control and modification of the chemical and topographical characteristics of materials of size less than 100 nm, down to 10 nm. The goal of this review is to discuss the role of titanium substrates as nanoscale surface modification tools for improving various aspects of implantology, including osseointegration and antibacterial properties. Techniques that can impart nanoscale topographical features to endosseous implants are described. Since the advent of nanotechnology, cellular specific functions, such as adhesion, proliferation, and differentiation, have been better understood. By applying these technologies, it is possible to direct cellular responses and improve osseointegration. Conversely, modulating surface features by nanotechnology could have the effect of decreased bacterial colonization.

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Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

Cited by 32 publications

References 39 publications

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

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