Antibacterial screening of plants from the Brazilian Atlantic Forest led to the identification of active compounds in <i>Miconia latecrenata</i> (DC.) Naudin

Rodrigues, Laís Azevedo; Almeida, Ayla das Chagas; Gontijo, Douglas Costa; Salustiano, Iorrana Vieira; Almeida, Alisson Andrade; Brandão, Geraldo Célio; Ribon, Andréa de Oliveira Barros; Leite, João Paulo Viana

doi:10.1080/14786419.2020.1802271

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…It is the aim of the BIOPROS group that has been implemented a large-scale project to produce and test extracts from the Atlantic Forest trees, where species are still rarely studied for their biological activity. The BIOPROS Extract Library is also screened in other biological targets such as antibacterial [24,25] , Anticholinesterase inhibitor [26] , Insecticidal [27] , and phytonematode control [28] .…”

Section: Resultsmentioning

confidence: 99%

Molecular bioprospecting of plant extracts: Experience report of the BIOPROS/UFV group in the search for antitumor compounds

Almeida

Leite

Simão

et al. 2022

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This manuscript discusses the experience of the research group BIOPROS (Molecular Bioprospecting in the Sustainable Use of Biodiversity) from the Federal University of Viçosa in the field of bioprospecting. We describe our experience on the search for antitumor compounds from the collection of extracts of native tree species from the Atlantic Forest biome. Presenting an interdisciplinary approach, integrating knowledge of forestry engineering, bioinformatics and natural products chemistry, the bioprospecting research of the BIOPROS group has innovated in the generation of pharmacochemical knowledge of native species of the Atlantic Forest. For the composition of the extract library a total of 220 plant species distributed in 57 botanical families and 140 genera were identified. 196 extracts from 49 plant species were produced; all of them evaluated for cytotoxic activity. By showing the process of obtaining a promising antitumor activity withanolides compounds from Athenaea velutina, a species hitherto little known to science, this manuscript shows our sequence of methodological steps used to unravel bioactive natural products from fragments of Atlantic Forest. The research follows the premises of the Convention on Biological Diversity, regarding the creation of strategies for the sustainable use of biodiversity.

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Section: Resultsmentioning

confidence: 99%

Molecular bioprospecting of plant extracts: Experience report of the BIOPROS/UFV group in the search for antitumor compounds

Almeida

Leite

Simão

et al. 2022

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“…Four major peaks were observed in the chromatographic profile (▶ Fig. 1 a) and a total of 14 possible withanolides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) were tentatively identified in FAv_5 (▶ Table 2), based on the chemical structure, fragmentation pattern, and by comparison with relevant literature data and the Dictionary of Natural Products (DNP) database. MS/MS spectra obtained for each compound are provided in the Supplementary material (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In line with our screening for naturally occurring plant-derived extracts, antibacterial and antimelanoma (i.e., murine melanoma B16F10 model) activities, as well as the presence of quinic acid derivatives and flavonoid glycosides, were recently reported in Athenaea velutina (Sendtn.) DʼArcy (Solanaceae) 5 , 6 . However, further research was needed to confirm this antitumor potential and correlate the respective bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

A Withanolide-rich Fraction of Athenaea velutina Induces Apoptosis and Cell Cycle Arrest in Melanoma B16F10 Cells

et al. 2021

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Athenaea velutina is a promising Brazilian shrub with cytotoxic and antimigratory properties against cancer cells. However, the mechanism of induction of cancer cell death and the compounds involved remain unknown. To ascertain these bioactive compounds, bioassay-guided fractionation was performed, alongside the appropriate in vitro tests. A withanolide-rich fraction (FAv_5) from the dichloromethane extract increased cytotoxic activity by 1.5-fold (IC50 = 2.1 µg/mL). Fourteen withanolide steroids were tentatively identified for the first time for this species by mass spectrometry coupled to liquid chromatography (LC MS/MS), including withanolide A, aurelianolide A, and aurelianolide B. FAv_5 significantly decreased cell proliferation, migration, and invasion with a selectivity index greater than 8 for B16F10 cells. Furthermore, flow cytometry with annexin V fluorescein isothiocyanate/propidium iodide (V-FITC/PI) staining showed FAv_5 to promote cell cycle arrest at the G0/G1-phase as well as apoptotic cell death. Overall, these findings highlight A. velutina as a source of withanolide-steroids that inhibit cancer cell proliferation through apoptosis and cell cycle blockade mechanisms. Details on the geographic distribution of A. velutina and species conservation strategies have also been highlighted.

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“…The aqueous extract from the leaves of M. latecrenata showed antibacterial activity against strains of Staphylococcus aureus, with an MIC value of 100.0 µg/mL [16]. Rodrigues et al [47] also demonstrated that the extract prepared from the leaves of M. latecrenata was promising in inhibiting the bacterial growth of S. aureus and Pseudomonas aeruginosa strains; in addition, it demonstrated synergism with ampicillin and tetracycline, respectively.…”

Section: Antimicrobial Potentialmentioning

confidence: 98%

The Genus Miconia Ruiz & Pav. (Melastomataceae): Ethnomedicinal Uses, Pharmacology, and Phytochemistry

et al. 2022

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Species of the genus Miconia are used in traditional medicine for the treatment of diseases, such as pain, throat infections, fever, and cold, and they used as depuratives, diuretics, and sedatives. This work reviewed studies carried out with Miconia species, highlighting its ethnomedicinal uses and pharmacological and phytochemical potential. This information was collected in the main platforms of scientific research (PubMed, Scopus, and Web of Science). Our findings show that some of the traditional uses of Miconia are corroborated by biological and/or pharmacological assays, which demonstrated, among other properties, anti-inflammatory, analgesic, antimutagenic, antiparasitic, antioxidant, cytotoxic, and antimicrobial activities. A total of 148 chemical compounds were identified in Miconia species, with phenolic compounds being the main constituents found in the species of this genus. Such phytochemical investigations have demonstrated the potential of species belonging to this genus as a source of bioactive substances, thus reinforcing their medicinal and pharmacological importance.

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Antibacterial screening of plants from the Brazilian Atlantic Forest led to the identification of active compounds in Miconia latecrenata (DC.) Naudin

Cited by 10 publications

References 17 publications

Molecular bioprospecting of plant extracts: Experience report of the BIOPROS/UFV group in the search for antitumor compounds

Molecular bioprospecting of plant extracts: Experience report of the BIOPROS/UFV group in the search for antitumor compounds

A Withanolide-rich Fraction of Athenaea velutina Induces Apoptosis and Cell Cycle Arrest in Melanoma B16F10 Cells

The Genus Miconia Ruiz & Pav. (Melastomataceae): Ethnomedicinal Uses, Pharmacology, and Phytochemistry

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