Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics

Abstract

The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO 3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO 3 : as AgNO 3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm 2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones.

A One-Pot Synthesis and Characterization of Antibacterial Silver Nanoparticle–Cellulose Film

“…Currently, the widely recognized antibacterial mechanisms of Ag-NPs include disrupting the normal function of the cell wall [5], interacting with the lipid components of the cell membrane to impede its normal function [6][7][8][9][10], inducing ROS free radicals to damage the cell membrane [11][12][13], damaging the DNA structure and inhibiting its related functions [14][15][16], binding with sulfhydryl groups of enzyme proteins to make cell inactive, and so on [17,18]. Ag-NPs inhibits the characteristics of simple preparation, broad-spectrum antibacterial, strong sterilization, and less prone to emerge drug resistance, which prompted it to be used as an antibacterial agent added to ceramics [19], coatings [20], textiles [21], films [22,23], and other raw materials to fabricate antibacterial materials.…”

Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens

“…The BCF embedded with nanobactericides were subjected to antibacterial activity according to the protocol described by Volova et al, 2018 with slight modification. In brief, small blocks of BCF with nanobactericides were excised under aseptic condition.…”

“…The synthesized nanobactericides were hybridized onto bacterial cellulose films. The bacterial cellulose is a network of cellulose fibrils with less than 100 nm which has resulted in unique properties thus forming one of the promising material (Volova et al, 2018). Scientific reports highlight the importance of BC due to its versatile properties, ease in large production via fermentation, inexpensive, generation in different shapes, absorptivity, elasticity, biocompatible, inert, hypoallergenity and mechanical properties (Volova et al, 2018).…”

“…The bacterial cellulose is a network of cellulose fibrils with less than 100 nm which has resulted in unique properties thus forming one of the promising material (Volova et al, 2018). Scientific reports highlight the importance of BC due to its versatile properties, ease in large production via fermentation, inexpensive, generation in different shapes, absorptivity, elasticity, biocompatible, inert, hypoallergenity and mechanical properties (Volova et al, 2018). In recent years, use of bacterial cellulose has rapidly expanded which has marked its application in biomedical sector to develop implants, catheters, scaffolds to tissue engineering, bio-composite etc (Moniri et al, 2017).…”

“…In recent years, use of bacterial cellulose has rapidly expanded which has marked its application in biomedical sector to develop implants, catheters, scaffolds to tissue engineering, bio-composite etc (Moniri et al, 2017). The biocompatibility of bacterial cellulose films is regarded as one of the best suit for biomedical applications to designed novel biomaterials such as artificial skin substitutes for treatment of wounds, ulcers and burns (Volova et al, 2018). In such situations, prevention of microbial infections is one of the top priority and bacterial cellulose lack antimicrobial potential (Hu and Hsieh, 2015;Heli et al,2016).…”