Antibacterial Properties of Chitosan Nanoparticles and Propolis Associated with Calcium Hydroxide against Single- and Multispecies Biofilms: An In Vitro and In Situ Study

Carpio-Perochena, Aldo del; Kishen, Anil; Felitti, Rafael; Bhagirath, Anjali Y.; Medapati, Manoj Reddy; Lai, Christopher; Cunha, Rodrigo Sanches

doi:10.1016/j.joen.2017.03.017

Cited by 67 publications

(56 citation statements)

References 30 publications

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“…Nonetheless, optimal concentration of brown propolis against E. faecalis is not known yet [50]. However, other factors such as type of the raw material, plant source, temperature zone, Though, P100 and P250 in this study showed antibacterial effect but it was not as effective as CPN that can be explained due to their poor penetrability in the dentinal tubule but when prepared as nanoparticles it enhances drug stability, treatment efficacy and penetration power compared to a pure drug solution [42,43].…”

Section: Discussionmentioning

confidence: 77%

“…Del Carpio-Perochena el al. also found that incorporating nanoparticles could potentially be beneficial when using interappointment intracanal medications because of their ability to kill bacteria in short-and long-term exposure [43]. Furthermore, factors such as zeta potential, poly dispersity index, encapsulation of nanoparticles and rate of release of the active ingredients attribute to the antibacterial effectiveness.…”

Section: Discussionmentioning

confidence: 96%

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Effectiveness of chitosan-propolis nanoparticle against Enterococcus faecalis biofilms in the root canal

Parolia

Kumar

Ramamurthy

et al. 2020

Preprint

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Background To determine the antibacterial effect of chitosan-propolis nanoparticle (CPN) as an intracanal medicament against Enterococcus faecalis biofilm in root canal. Methods 240 extracted human teeth were sectioned to obtain 6mm of the middle third of the root. The root canal was enlarged to an internal diameter of 0.9mm. The specimens were inoculated with E. faecalis for 21 days. Following this, specimens were randomly divided into eight groups ( n=30 ) according to the intracanal medicament placed: group I: saline, groupII: chitosan, group III: propolis100 µg/ml (P100), group IV: propolis 250 µg/ml (P250), group V: chitosan-propolis nanoparticle 100µg/ml (CPN100), group VI: chitosan-propolis nanoparticle 250 µg/ml (CPN250), group VII: calcium hydroxide(CH) and group VIII: 2% chlorhexidine (CHX) gel. Dentine shavings were collected at 200 and 400 μm depths, and total numbers of CFUs were determined at the end of day one, three and seven. The non-parametric Kruskal Wallis and Mann-Whitney tests were used to compare the differences in reduction of CFUs between all groups and probability values of P < 0.05 were set as the reference for statistically significant results. The scanning electron microscope (SEM) and confocal laser scanning microscopy (CLSM) were also performed after exposure to CPNs. The effectiveness of CPNs were also evaluated against E. faecalis isolated obtained from patients having failed root canal treatment. Results Reduction in the number of colony‐forming units was statistically significant in all groups compared to saline (p <.05). On day one and three, at 200 and 400-μm, CPN250 showed significant reduction of CFUs compared to all other groups (p <.05), while CPN100 was significantly better than other groups (p <.05) except CPN250 and CHX. On day seven, at 200-μm CPN250 showed significant reduction of CFUs compared to all other groups (p <.05) except CPN100 and CHX, while at 400 μm CPN250 showed similar effectiveness as CPN100, CH and CHX. SEM and CLSM images also showed the maximum reduction of E. faecalis with CPN250. Conclusion CPN250 was the most effective in reducing E. faecalis colonies on day one, three at both depths and at day seven CPN250 was equally effective as CPN100 and CHX.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 96%

Effectiveness of chitosan-propolis nanoparticle against Enterococcus faecalis biofilms in the root canal

Parolia

Kumar

Ramamurthy

et al. 2020

Preprint

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“…74 Otherwise, EEP showed synergistic antimicrobial effect with antibiotics {oxacillin, 27 clarithromycin, 75 cefazolin, 76 ciprofloxacin 50 cefixime 77 and many different antibiotics 27,53,66,69 } or essential oils of ginger, 78 garlic, 79 cinnamon, 80 clove, 78 or chitosan. 81 The study concluded that Egyptian fennel honey and EEP have potent antimicrobial activity against biofilm producing MRSA, while both apiproducts revealed more potency and synergy will be achieved against these foodborne pathogens. It is recommended to use the nutritive materials (fennel honey & EEP) as food preservatives against foodborne pathogens especially biofilm producing MRSA.…”

Section: Resultsmentioning

confidence: 97%

Egyptian fennel honey and/or propolis against MRSA harboring both mecA & icaAgenes

Sm¹,

Mf²,

Mm³

et al. 2018

IJCAM

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“…ese results bring a negative perspective to the prognosis of endodontically treated teeth, especially in cases of chronic apical periodontitis due to secondary infections because they are frequently associated with the presence of E. faecalis [25]. Few studies in the literature have evaluated the combined use of phytotherapeutic agents and calcium hydroxide in dental applications [7][8][9][10][11].…”

Section: Discussionmentioning

confidence: 99%

“…Several phytotherapeutic compounds have been studied in pure form or combined with calcium hydroxide in order to potentiate the effects of the latter, improving the prognosis of endodontic treatment [7][8][9][10][11]. Plant extracts such as the essential oils of rosemary (Rosmarinus officinalis L.), ginger (Zingiber officinale), bergamot (Citrus aurantium bergamia), and copaiba (Copaifera officinalis) have been shown to possess antimicrobial, anti-inflammatory, antifungal, analgesic, antioxidant, and healing properties [8,12,13].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Rosmarinus officinalis, Zingiber officinale, Citrus aurantium bergamia, and Copaifera officinalis Alone and in Combination with Calcium Hydroxide against Enterococcus faecalis

Silva

Alves

Silva

et al. 2019

BioMed Research International

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is study aimed to evaluate the efficacy of different concentrations of essential oils combined with calcium hydroxide against Enterococcus faecalis. irteen experimental groups were formed: NC (negative control); PC (positive control); GC (growth control); SC (sterilization control); RO (Rosmarinus officinalis); ROH (calcium hydroxide + RO); ZO (Zingiber officinale); ZOH (calcium hydroxide + ZO); AB (Citrus aurantium bergamia); ABH (calcium hydroxide + AB); CO (Copaifera officinalis); COH (calcium hydroxide + CO); DWH (calcium hydroxide and distilled water). After reconstitution of the E. faecalis strain, microdilution testing was performed to define the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). e data were tabulated in an Excel spreadsheet, and the MIC and MBC were calculated in accordance with the Bacteriological Analytical Handbook. MICs in the range of 0 to 100 mg/ml were only observed in the ROH group. e RO, ROH, AB, ZO, and ZOH presented absolute data for MBC. Bacterial growth was detected in the DWH group at all concentrations tested. e combination of the essential oils tested here with calcium hydroxide appears promising as an intracanal medication in endodontic treatment because of its effectiveness against Enterococcus faecalis. Essential oils are important in endodontic therapy since calcium hydroxide, the gold standard intracanal medication, is not effective against E. faecalis.

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Antibacterial Properties of Chitosan Nanoparticles and Propolis Associated with Calcium Hydroxide against Single- and Multispecies Biofilms: An In Vitro and In Situ Study

Cited by 67 publications

References 30 publications

Effectiveness of chitosan-propolis nanoparticle against Enterococcus faecalis biofilms in the root canal

Effectiveness of chitosan-propolis nanoparticle against Enterococcus faecalis biofilms in the root canal

Egyptian fennel honey and/or propolis against MRSA harboring both mecA & icaAgenes

Antibacterial Activity of Rosmarinus officinalis, Zingiber officinale, Citrus aurantium bergamia, and Copaifera officinalis Alone and in Combination with Calcium Hydroxide against Enterococcus faecalis

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