Antibacterial Potential of <i>Jatropha</i> sp. Latex against Multidrug-Resistant Bacteria

Prastiyanto, Muhammad Evy; Tama, Prayoda Deri; Ananda, Ninda; Wilson, Wildiani; Mukaromah, Ana Hidayati

doi:10.1155/2020/8509650

Cited by 22 publications

(24 citation statements)

References 11 publications

(12 reference statements)

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“…Methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum beta-lactamase-producing Escherichia coli (ESBL-producing E. coli ) and the agar well diffusion method were used to evaluate the antibacterial activity of the isolated compounds [ 29 ]. The strains were cultured in nutrient broth at 37 °C for 18 h and diluted with steriled 0.9% NaCl to obtain the 1.5 × 10 8 CFU/mL bacterial solution.…”

Section: Methodsmentioning

confidence: 99%

Flavones from Combretum quadrangulare Growing in Vietnam and Their Alpha-Glucosidase Inhibitory Activity

Dao¹,

Nguyen²,

Nguyen³

et al. 2021

Molecules

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Combretum quadrangulare Kurz is widely used in folk medicine in Eastern Asia and is associated with various ethnopharmacological properties including hepatoprotective, antipyretic, analgesic, antidysenteric, and anthelmintic activities. Previous phytochemical investigations reported the presence of numerous triterpenes (mostly cycloartanes, ursanes, lupanes, and oleananes) along with dozens of flavonoids. However, the extracts of C. quadrangulare and isolated flavonoids have not been evaluated for their alpha-glucosidase inhibition. In the frame of our efforts dedicated to the chemical investigation of Vietnamese medicinal plants and their biological activities, a phytochemical study of the MeOH extract of the leaves of C. quadrangulare using bioactive guided isolation was undertaken. In this paper, the isolation and structure elucidation of twelve known compounds, 5-hydroxy-3,7,4′-trimethoxyflavone (1), ayanin (2), kumatakenin (3), rhamnocitrin (4), ombuin (5), myricetin-3,7,3′,5′-tetramethyl ether (6), gardenin D (7), luteolin (12), apigenin (13), mearnsetin (14), isoorientin (15), and vitexin (16) were reported. Bromination was applied to compounds 2 and 3 to provide four new synthetic analogues 8–11. All isolated and synthesized compounds were evaluated for alpha-glucosidase inhibition and antibacterial activity. Compounds 4 and 5 showed moderate antibacterial activity against methicillin-resistant Staphylococcus aureus while others were inactive. All compounds failed to reveal any activity toward extended spectrum beta-lactamase-producing Escherichia coli. Compounds 2, 4, 6–9, and 11–14 showed good alpha-glucosidase inhibition with IC50 values in the range of 30.5–282.0 µM. The kinetic of enzyme inhibition showed that 8 and 11 were noncompetitive type inhibition against alpha-glucosidase. In silico molecular docking model indicated that compounds 8 and 11 were potential inhibitors against enzyme α-glucosidase.

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Section: Methodsmentioning

confidence: 99%

Flavones from Combretum quadrangulare Growing in Vietnam and Their Alpha-Glucosidase Inhibitory Activity

Dao¹,

Nguyen²,

Nguyen³

et al. 2021

Molecules

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“…The MIC was determined as the lowest concentration of extract that completely inhibited the growth of the MRSA detected by the unaided eye (Valle et al 2015). MBC was defined as the concentration which exhibits zero growth of the colonies compared to the culture of the initial inoculum of the same strain (Manilal et al 2020;Prastiyanto et al 2020b). The Wells were subcultured using a 10 µL inoculating loop on to a BAP at (35 ± 2) o C for 16-20 h incubation.…”

Section: Minimum Inhibitory Concentrations (Mic) and Minimum Bactericmentioning

confidence: 99%

“…Infections caused by MRSA bacteria consume more health care resources than are consumed by patients infected with non-resistant strains of the same bacteria (Word Health Organization 2018). In consequence, novel antibacterial agents are needed from natural biological sources (Asagabaldan et al 2019;Lestari et al 2019;Prastiyanto et al 2020b;2020a, 2020c. Neutral proteoglycans derived from the P. ostreatus mycelia have been reported that could be used as immunomodulators and anticancer agents (Sarangi et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus

et al. 2020

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Abstract. Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of this work is to identify the potential effects of methanolic extracts from four species of the Pleurotus genus cultivated in Indonesia on nasopharynx cancer (Raji cell line), and to investigate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). This study investigates four species members of Pleurotus (Pleurotus ostreatus, P. cystidiosus, P. flabellatus, and P. pulmonarius var. stechangii). Dry Samples were extracted with methanol to yield crude extracts. Cytotoxicity screening was conducted using MTT assay of dry extracts, while antibacterial activity was calculated based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Mueller–Hinton broth, via the microdilution method. Compounds were analyzed using thin-layer chromatography (TLC). P. flabellatus provided the highest yield of dry extract (20.8%) with the lowest value of IC50 (556.226 µg/mL) compared to the three other species investigated. Antibacterial activity was calculated as MIC and MBC values against MRSA by the P. flabellatus extract which reached 6.25 mg/mL and 250 mg/mL, respectively. The result of TLC of the dry extract of P. flabellatus revealed the presence of terpenoids. P. flabellatus has the potential to be developed as both an anti-cancer and an antibacterial agent, especially against Raji cells and MRSA strains. However, further in vivo research and discovery of the modes of action involved are still needed to shed light on these effects. Studies can provide new information about the benefits of Pleurotus as a source of natural anticancer and antibacterial compound.

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“…Utilization of plants as medicine has been done since ancient times, (Al-dhabi and Arasu 2016; Elango et al 2016;Manh et al 2017). Many plants are used as traditional medicines having anticancer (Adnan and Ahmed 2019), antioxidant (Diniyah et al 2020), antibacterial (Prastiyanto et al 2020a;2020b) and antifungal (Kader et al 2011) properties. The members in Zingiberaceae have been recognized in uses of medicine such as antibacterial (Wibowo et al 2020), antifungal (Akter et al 2018), anticancer (Mahomoodally et al 2019), anti-arthritic (Murugesan et al 2020) and antioxidant (Ghafoor et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum

et al. 2021

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Abstract. Prastiyanto ME, Rohmah N, Efendi L, Arifin R, Wardoyo FA, Wilson W, Mukaromah AH, Dewi SS, Darmawati S. 2021. Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum. Biodiversitas 22: 1509-1513. Fungal infections have now become serious health issues. One of the strategies to avoid the problems of fungal infections is by using natural product from plants that are effective against many human pathogenic fungi. The study portrayed the use of the extracts of plant rhizomes as the alternatives to fight against number of human pathogenic fungi. This research aimed to investigate the antifungal activities of crude ethanol extract of five member of the family Zingiberaceae (Curcuma longa, Alpinia galanga Zingiber officinale. var. rubrum, Zingiber officinale var. officinarum and Zingiber officinale var. amarum), which are widely used as folk medicines against Candida albicans and Trichophyton rubrum. Crude ethanol extracts of five members of Zingiberaceae were evaluated for their antifungal activities and the results were calculated based on the zones of inhibition using the diffusion method. The extract showed antifungal activity against Candida. albicans in the agar well diffusion assay (10.2-27.1 mm inhibition diameter) and against T. rubrum (27.3-44.3 mm inhibition diameter). The data have revealed that all rhizomes have the potential to be developed as antifungal agents, particularly against C. albicans and T. rubrum. Studies on the antifungal activity against yeast-like (C. albicans) and filamentous (T. rubrum) can provide new information about the benefits of members Zingiberaceae as a source of natural antifungal. Researchers can select the type of rhizome that has more potential for further extraction to obtain pure compounds that can be used as antifungals.

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Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria

Cited by 22 publications

References 11 publications

Flavones from Combretum quadrangulare Growing in Vietnam and Their Alpha-Glucosidase Inhibitory Activity

Flavones from Combretum quadrangulare Growing in Vietnam and Their Alpha-Glucosidase Inhibitory Activity

Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus

Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum

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