Antibacterial potential of a small peptide from Bacillus sp. RPT-0001 and its capping for green synthesis of silver nanoparticles

Abstract

Infirmity and death from diseases caused by unsafe food are a continual hazard to communal health safety and socio-economic growth throughout the world. Chemical preservatives are associated with health hazards and toxicity issues. In the study reported here, 200 soil isolates from Western Himalayan region in India were screened for potential antibacterial activity against food-borne pathogens. This study led to the isolation of a bacterial strain belonging to the Genus Bacillus and was designated as RPT-0001. The associated antibacterial activity was sensitive to pronase E treatment. Bioassay-guided fractionation using reverse phase high performance liquid chromatography (RP-HPLC) led to isolation of the antibacterial peptide designated as RPT-0001. The molecular weight of RPT-0001 was determined by electro-spray ionization mass spectroscopy (ESI-MS) as 276.9 Da. RPT-0001 was inhibitory to both Gram-negative and Grampositive food-borne bacteria tested. The characteristics of RPT-0001 do not match with that of any other known antibacterial peptides produced by Bacillus sp. or related genera. Purified RPT-0001 was successfully used in synthesis of silver nanoparticles effective against food-borne pathogenic bacteria. The antibacterial peptide and silver nanoparticles synthesized utilizing it as a capping and reducing agent hold promising potential in food preservation, in packaging material and as a therapeutic agent in the treatment of foodborne infections.

Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review

“…Bacteriocin is a small heat-stable peptide which has a bactericidal effect on pathogenic microorganisms [112]. The bacteriocin derived from the microbes could act as a reducing agent for the synthesis of metal NPs [113]. In addition, previous study has proved that this small peptide could also bind to the surface of NPs as a capping agent which in turn enhance the antimicrobial effects of metal NPs [112–116].…”

Bacteriocin‐capped silver nanoparticles for enhanced antimicrobial efficacy against food pathogens

“…These biosynthesis results are in consonance with similar studies conducted by different researchers. The absorption peak at 420 nm was reported by Patil et al [11] using Bacillus species RPT0001 in conjugation with SNPs. Singh et al [24] reported absorption peak at 450 nm using partially purified bacteriocins extracted from lactic acid bacteria.…”

“…The production and partial purification of bacteriocin were carried out according to the method described by Patil et al [11], and Sharma et al [17] with slight modifications. An overnight grown culture of L. brevis MTCC 4463 (standard strain) and the isolate L. herbarum was centrifuged at 10,000 rpm for 15 min at 4°C and the cell-free supernatant was collected.…”

“…However, among these, silver nanoparticles (SNPs) are the ones which have been the most investigated and are known for their high antimicrobial responses. Silver in nano‐form has exceptional magnetic, antibacterial and antifungal properties [11, 12]; and the SNPs are known to impart higher toxicity against both Gram‐positive and Gram‐negative bacteria in comparison to the other metallic nanoparticles. Remarkably, although they have been observed to be toxic to microorganisms; but at the same time, they have also been touted to be the least toxic to mammalian cells [13].…”

Advances in Lipid and Metal Nanoparticles for Antimicrobial Peptide Delivery

“…BiomassSpherical50 and 100 M. smegmatis M. smegmatis and M. marinum [182]Bacitracin A and polymyxin EAgNPsSpherical3.1 E. coli , B. amyloliquefaciens , P. aeruginosa and S. aureus Promotes healing of infected wounds[181]G 3 R 6 TATAgNPs-citrateAgNPs-SDS and Au@Ag-BSATriangularSpherical30–7030 B. subtilis , E. coli and C. albicans [165]LL-37CYS-modified (LL37-SH)AgNPsSpherical5.3 ± 1.8No anti-proliferative effect on primary skin cells; promotes wound healing, preventing potential infection by E. coli , Staphylococcus epidermidis , S. aureus , and free living and biofilm forms of P. aeruginosa [210]NisinAgNPs incorporated in poly( d , l -lactide) (PDLLA) and poly(ethylene oxide) (PEO) nanofibers-21.81 ± 5.5 P. aeruginosa , Klebsiella pneumoniae , E. coli , S. typhimurium and S. aureus [211]RPT-0001AgNPsSpherical20–30Against food-borne bacterial pathogens: L. monocytogenes , Cronobacter sakazakii , S. enterica subsp. enterica and E. coli [212]IndolicidinCOOH-functionalized AuNPsSpherical3Immuno suppressive action by downregulation of IFNβ expression and increase of IL-10 in RAW264.7 murine macrophage cells and THP-1 human monocyte cell lines…”