2002
DOI: 10.1016/s0005-2736(02)00470-4
|View full text |Cite
|
Sign up to set email alerts
|

Antibacterial peptide pleurocidin forms ion channels in planar lipid bilayers

Abstract: Pleurocidin, a 25-residue alpha helical cationic peptide, isolated from skin mucous secretions of the winter flounder, displays a strong anti-microbial activity and appears to play a role in innate host defence. This peptide would be responsible for pore formation in the membrane of bacteria leading to lysis and therefore death. In this study, we investigated the behaviour of pleurocidin in different planar lipid bilayers to determine its mechanism of membrane permeabilisation. Macroscopic conductance experime… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

10
47
0

Year Published

2003
2003
2015
2015

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 57 publications
(57 citation statements)
references
References 39 publications
10
47
0
Order By: Relevance
“…Previous studies showed that the pleurocidin isolated from the winter flounder, which migrates from its habitat of brackish water (0.8% salinity) to seawater (3.3% salinity), was naturally resistant to high concentrations of salt (5,25). Importantly, the pleurocidin did not depend on high salt concentrations for killing activity (5,28). The evidence from our study suggests that IMB-2 retains a level of salinity resistance similar to that of its ancestor peptide.…”
Section: Vol 55 2011supporting
confidence: 53%
“…Previous studies showed that the pleurocidin isolated from the winter flounder, which migrates from its habitat of brackish water (0.8% salinity) to seawater (3.3% salinity), was naturally resistant to high concentrations of salt (5,25). Importantly, the pleurocidin did not depend on high salt concentrations for killing activity (5,28). The evidence from our study suggests that IMB-2 retains a level of salinity resistance similar to that of its ancestor peptide.…”
Section: Vol 55 2011supporting
confidence: 53%
“…Moronecidin is a 22-amino acid, linear, cationic peptide with an amphipathic, ␣-helical structure that exhibits a more potent, broader spectrum of bactericidal activity than hepcidin (5). Peptides like moronecidin are thought to aggregate and interact with negatively charged bacterial membrane components and to disrupt them by forming pores or solubilizing the membrane via a "detergent" effect (53)(54)(55). This direct membrane disruption is believed to kill the bacterium by creating osmotic imbalance and loss of cytoplasm.…”
Section: Discussionmentioning
confidence: 99%
“…Pleurocidin was isolated from the winter flounder Pleuronectes americanus (Walbaum) in 1997 (9), and its activity has been under investigation by several research groups (13,20,21,23,28). Also, pleurocidin genes have been cloned, and their genetic organization has been described (8, 10; S. E. Douglas, A. Patrzykat, J. Pytyck, and J. W. Gallant, submitted for publication).…”
mentioning
confidence: 99%