Antibacterial Evaluation and Phytochemical Analysis of Selected Medicinal Plants against Some Pathogenic Enteric Bacteria in Gozamin District, Ethiopia

Fentahun, M; B, Ayele Yilkal; Amsalu, N; Alemayehu, Addisu; Amsalu, G

doi:10.4172/2329-6887.1000244

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Seid and Ayisha 51 were also reported the same finding in Ethiopia. This is associated with the extracting efficiency of methanol to liberate most of the biologically active phytochemical compounds like flavonoids, tannins, and carotenoids, which agrees with the ideas of Bakari et al 53 and Felhi et al 54 On the other hand, in this study, Gram-positive bacterial strains were relatively less sensitive to both the petroleum ether and methanol extracts, which is in agreement with the results of Fentahun et al 30 The MIC determination test revealed that a different minimum concentration of the crude extract was gained in three of the plants that could inhibit the growth of the reference bacteria. The least MIC value was recorded for methanol extract of R. chalepensis against P. aeruginosa and E. coli.…”

supporting

confidence: 93%

“…Ethiopia has a long history of a traditional health care system, but studies on traditional medicinal plants (TMP) have been limited in comparison to the country's multiethnic, cultural, and flora diversity. 30 Also, the use of medicinal plants to treat infections is an old practice in large parts of Ethiopia to solve health problems for livestock and humans. [31][32][33][34][35][36] However, most of these experiences are not documented and supported by scientific experiments.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Screening and in-vitro Evaluation of Antibacterial Activities of Echinops amplexicaulis, Ruta chalepensis and Salix subserrata Against Selected Pathogenic Bacterial Strains in West Shewa Zone, Ethiopia

Marami¹,

Dilba²,

Babele³

et al. 2021

JEP

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Background: Although traditional healers in Ethiopia have a long history of using medicinal plants to treat diseases in animals and humans, studies on the antibacterial activities and potential bioactive ingredients of most medicinal plants have been insufficient. Therefore, this study aimed to evaluate the in-vitro antibacterial activities and to screen phytochemical constituents of selected medicinal plants against reference bacterial strains. Methods: The fresh and healthy roots of Echinops amplexicaulis, fruits of Ruta chalepensis, and leaves of Salix subserrata were collected from West Shewa Zone, Ethiopia. Agar well diffusion and agar dilution methods were used to evaluate antibacterial activities and minimum inhibitory concentrations (MIC). All the crude plant extracts were tested against Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Pseudomonas aeruginosa at concentrations of 100, 50, and 25 mg/mL in each triplet (3x). MIC of crude extracts ranging from 1.5625 to 12.50 mg/mL was applied to all bacterial strains. The positive control was ciprofloxacin disk (5 μg) and the negative control was 5% dimethyl sulfoxide. The presence of secondary metabolites of each crude extract was screened. The group means comparisons were done using one-way ANOVA and results were presented as mean ± standard deviation. Results: Although all selected plant extracts had shown antibacterial activities, methanol extracts had a greater zone of inhibition against all reference bacterial strains when compared to petroleum ether extracts. The growth of P. aeruginosa was inhibited at a minimum concentration of both methanol and petroleum extracts (1.5625 mg/mL) when compared to the remaining bacterial strains. Phytochemical screening showed that saponins and alkaloids were found in all crude plant extracts, while phytosterol was meager. Conclusion:This study revealed that all tested plants had significant secondary metabolites and antibacterial activities against reference bacterial strains.

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supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening and in-vitro Evaluation of Antibacterial Activities of Echinops amplexicaulis, Ruta chalepensis and Salix subserrata Against Selected Pathogenic Bacterial Strains in West Shewa Zone, Ethiopia

Marami¹,

Dilba²,

Babele³

et al. 2021

JEP

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“…To 0.5ml of the extract of plant materials in a test tube, 5 ml of distilled water was added and the mixture was vigorously shaken. Formation of froth persistent for 30 minutes confirms the presence of saponins [21].…”

Section: Methodsmentioning

confidence: 94%

Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts of Sida rhombifolia’s Aerial Part

Debalke

Birhan

Kinubeh

et al. 2018

The Scientific World Journal

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Infectious diseases are the critical problems of the world as a result of the emergence of different antimicrobial resistant microorganisms due to several reasons like misuses and repeating uses of antibiotics. Because of this, searching of new treatment method is important from natural substances to against those infectious diseases in both human and animals' aspects. Among those plants, Sida rhombifolia has various roles against those infectious diseases through its different phytochemical components. The objectives of this study were assessing the antibacterial activity of the aqueous-methanol extract of the plant's aerial part and knowing the phytochemical constituents of the plant. Preliminary phytochemical screening revealed that the extract of S. rhombifolia's aerial part possesses flavonoids, alkaloids, polyphenols, and quinines. In addition to this, the antibacterial activity of the plant extract was evaluated on five pathogenic bacteria species using agar well diffusion method at different concentrations of plant extracts. Minimum inhibition concentration and minimum bactericidal concentration determinations were done by tetrazolium chloride microtiter dilution assay. The inhibition zone of mean diameters ranging from 0.00 to 7.67mm against all test bacteria was significantly (p<0.05) much less than that of the positive control Chloroamphinicole (30μg/disc) with the range of 14.33mm-15mm of inhibition zone of diameters. The inhibition zones of the tested bacteria at the concentration of 62.5mg/ml were much less than the higher concentration (500mg/ml) and significantly different (p<0.05), whereas the MIC value ranges from 4.62 to 97.22mg/ml and the MBC value ranges from 4.62 to 125.00mg/ml. Even if the plant extract showed antibacterial activity, it was lower than that of other solvent extraction methods; so other solvent extraction methods and fractionates must be conducted to investigate the antibacterial activities of the plant extract on different bacterial strains and species that cause different diseases.

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“…However, some studies have reported the soaking method in which the crude extracts were dissolved in TWEEN-20 solvent [to emulsify carrier oil in water [123]] and 10% stock solutions were prepared. The blotting paper discs (6 mm diameter) were soaked in various dilute solvent extracts and dried for 5 min to avoid the flow of extracts in the test media [66,124]. The following step is air drying, maintaining the sterility of material.…”

Section: Agar Diffusion and Dilution Methodsmentioning

confidence: 99%

Testing the Antimicrobial Characteristics of Wood Materials: A Review of Methods

et al. 2020

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Some wood species have antimicrobial properties, making them a better choice over inert surfaces in certain circumstances. However, the organic and porous nature of wood raises questions regarding the use of this material in hygienically important places. Therefore, it is reasonable to investigate the microbial survival and the antimicrobial potential of wood via a variety of methods. Based on the available literature, this review classifies previously used methods into two broad categories: one category tests wood material by direct bacterial contact, and the other tests the action of molecules previously extracted from wood on bacteria and fungi. This article discusses the suitability of these methods to wood materials and exposes knowledge gaps that can be used to guide future research. This information is intended to help the researchers and field experts to select suitable methods for testing the hygienic safety and antimicrobial properties of wood materials.

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Antibacterial Evaluation and Phytochemical Analysis of Selected Medicinal Plants against Some Pathogenic Enteric Bacteria in Gozamin District, Ethiopia

Cited by 6 publications

References 13 publications

Phytochemical Screening and in-vitro Evaluation of Antibacterial Activities of Echinops amplexicaulis, Ruta chalepensis and Salix subserrata Against Selected Pathogenic Bacterial Strains in West Shewa Zone, Ethiopia

Phytochemical Screening and in-vitro Evaluation of Antibacterial Activities of Echinops amplexicaulis, Ruta chalepensis and Salix subserrata Against Selected Pathogenic Bacterial Strains in West Shewa Zone, Ethiopia

Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts of Sida rhombifolia’s Aerial Part

Testing the Antimicrobial Characteristics of Wood Materials: A Review of Methods

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