Antibacterial Efficacy of the Compound Preparation of Traditional Chinese Drug on System Fungi Reside in Faecal Specimen of Pigeon

Abstract

To investigate the efficacy of the compound preparation of traditional Chinese drug which is named KZY-2) on system fungi reside in faecal specimen of pigeon, the 66 faecal specimen of pigeon were collected from columbary of local park and residents.Each specimen which weigh 2.0g was mixed with 10ml sterile saline and the supernatant which volume was 1ml was incubated with the same volume drug which concentration were 200mɡ/ml,100mɡ/ml and 50mɡ/ml respectively at 37°C.After 24h,48h and 72h, the isolated positive rates of Cryptococcus neoformans and Candida albicans were calculated. Candida albicans was identified by gram staining, budding development, chlamydospore formation, sugar fermented test,sugar assimilated test and Cryptococcus neoformans was identified by gram staining,sugar fermented test,sugar assimilated test,urease tests,caffeic acid test, Hiss capsule staining.Results showed that the isolated positive rates of Cryptococcus neoformans and Candida albicans were 27.3%（18/66） and 16.7%（11/66） without drug action, KZY-2 could reduce the isolated positive rates of Cryptococcus neoformans and Candida albicans(P<0.05 and P<0.05).The drug concentration and action time could significantly affect the isolated positive rate which was negative correlation between them, higher drug concentration and longer incubation time,more lower the positive rate, especially the isolated positive rates of Cryptococcus neoformans are reduced 77.7%（6.1/27.3）which incubated with 200mɡ/ml drug after 72h compared with the control group.These results indicate The KZY-2 have good germicidal efficacy on Cryptococcus neoformans and Candida albicans isolated from faecal specimen of pigeon.

Effect of Room Air Disinfection about Disinfectant Maken from Traditional Chinese Medicine

“…Air is important for survival of human and important medium for the spread of disease, school is not only the base for teacher working and learning, but also the zone for student studying and living, so air quality of campus is closely related to the health of teacher and student [1].Because teacher and student mostly stay in the classroom and laboratory in which microorganism especially the pathogenic microorganism is spread through dust and droplet. Therefore, it's important for management of campus environmental health, protection of school personnel health, prevention and control of epidemic diseases to know the case about room air pollution by microorganism in classroom and laboratory and discuss the good practices of air disinfection.In order to look for the air disinfection made from traditional chinese medicine which is nontoxic, no stimulus, no corrosion role, and have good effect, the six experiment zone, which were classroom, microorganism teaching laboratory, teacher's office, the corridor of out-patient department, in-patient department hall and department of dermatology, were selected to observe the case about room air pollution and the disinfection effect after disinfected and compared prescription made from traditional chinese medicine and proven good antifungal and nontoxic on human and animal with ultraviolet ray [2][3][4][5].…”