Antibacterial efficacy of ethanolic extract of Camellia sinensis and Azadirachta indica leaves on methicillin-resistant Staphylococcus aureus and shiga-toxigenic Escherichia coli

Zihadi, Asief Hossain; Rahman, Mohammad Mizanur; Talukder, Sudipta; Hasan, Md. Mehedi; Nahar, Samsun; Sikder, Mahmudul Hasan

doi:10.5455/javar.2019.f340

Cited by 18 publications

(17 citation statements)

References 31 publications

(34 reference statements)

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“…19 The antibacterial properties of green tea extract were also verified by Zihadi et al, that reported MIC values of 15.625 and 31.25 mg.mL −1 against methicillin-resistant Staphylococcus aureus (MRSA) and shiga-toxigenic Escherichia coli (STEC), respectively. 38 On the other hand, Orak et al, using the same agar well-diffusion method used in the present work for microbiological analysis, also did not verified any antimicrobial activity of green tea against E. coli ATCC 25922. 39 But an inhibition halo was observed in this work for S. aureus ATCC 25923.…”

Section: Resultscontrasting

confidence: 62%

Blend of cassava starch and high-density polyethylene with green tea for food packaging

Romagnolli

Leite

Rodrigues

et al. 2020

Polymers from Renewable Resources

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Plastic packagings are widely used for several food products. Considering the relatively short service lifetime of this application, it is important to perceive in the search of eco-friendly alternatives to this market, such as polymers from renewable sources, as thermoplastic starch and “green” polyethylene. The incorporation of an antibacterial agent to the packaging can extend food shelf life. Camellia sinensis is a plant with known antibacterial properties used in the preparation of “green tea.” In the present work, green tea was incorporated to a blend of cassava thermoplastic starch and high-density polyethylene (HDPE) by melt extrusion, aiming application as active packaging. Films were obtained by thermopressing and characterized through infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and tensile tests. Their antibacterial properties were evaluated against Staphylococcus aureus and Escherichia coli. The results indicated that the material developed has potential for food packaging applications. Moreover, the methodology applied for green tea incorporation in the Starch/HDPE films can be extended for many extracts from natural components, contributing to the advancement of research in the development of active packaging for food products. To the best of our knowledge, no previous work studied the properties of starch/HDPE blend with green tea.

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Section: Resultscontrasting

confidence: 62%

Blend of cassava starch and high-density polyethylene with green tea for food packaging

Romagnolli

Leite

Rodrigues

et al. 2020

Polymers from Renewable Resources

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“…In contrast, the Stephania suberosa Forman extract (SSE) against ampicillin-resistant S. aureus showed higher MIC with 4 mg/ml [34]. High MIC was also noted in the olive oil polyphenol extract [41], Camellia sinensis, and Azadirachta indica leaves extracts [42] against S. aureus. This indicates that Photorhabdus extracts are more effective than SSE, olive oil polyphenol extract, Camellia sinensis, and Azadirachta indica leaves extracts.…”

Section: Plos Onementioning

confidence: 99%

Antibacterial activity of Xenorhabdus and Photorhabdus isolated from entomopathogenic nematodes against antibiotic-resistant bacteria

et al. 2020

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Xenorhabdus and Photorhabdus, symbiotically associated with entomopathogenic nematodes (EPNs), produce a range of antimicrobial compounds. The objective of this study is to identify Xenorhabdus and Photorhabdus and their EPNs hosts, which were isolated from soil samples from Saraburi province, and study their antibacterial activity against 15 strains of drug-resistant bacteria. Fourteen isolates (6.1%), consisting of six Xenorhabdus isolates and eight Photorhabdus isolates, were obtained from 230 soil samples. Based on the BLASTN search incorporating the phylogenetic analysis of a partial recA gene, all six isolates of Xenorhabdus were found to be identical and closely related to X. stockiae. Five isolates of Photorhabdus were found to be identical and closely related to P. luminescens subsp. akhurstii. Two isolates of Photorhabdus were found to be identical and closely related to P. luminescens subsp. hainanensis. The remaining isolate of Photorhabdus was found to be identical to P. asymbiotica subsp. australis. The bacterial extracts from P. luminescens subsp. akhurstii showed strong inhibition the growth of S. aureus strain PB36 (MSRA) by disk diffusion, minimal inhibitory concentration, and minimal bactericidal concentration assay. The combination between each extract from Xenorhabdus/Photorhabdus and oxacillin or vancomycin against S. aureus strain PB36 (MRSA) exhibited no interaction on checkerboard assay. Moreover, killing curve assay of P. luminescens subsp. akhurstii extracts against S. aureus strain PB36 exhibited a steady reduction of 10 5 CFU/ml to 10 3 CFU/ml within 30 min. This study demonstrates that Xenorhabdus and Photorhabdus, showed antibacterial activity. This finding may be useful for further research on antibiotic production.

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“…For example, Zihadi et al (2019) have investigated the antibacterial potential of ethanolic extract of Green tea (Camellia sinensis) and Neem (Azadirachta indica) leaves on methicillin-resistant Staphylococcus aureus (MRSA) and Shiga-toxigenic Escherichia coli (STEC). Results obtained by Zihadi et al (2019) revealed that the maximum zone diameter of inhibition value was observed for green tea against MRSA (7.5 mm) and minimum for neem (4.9 mm). Moreover, the highest zone diameter of inhibition against STEC was also for green tea and the combination of green tea and neem (4.5 mm).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Efficacy of Ethanolic Extracts Obtained from Leaves of Camellia japonica L. Cultivars against Escherichia coli Strain

2021

Agrobiodivers Improv Nutr health life Qual

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The present study was aimed to determine the antibacterial activity of six plants, i.e. Camellia japonica L. (cultivars Kramer's Supreme, C.M. Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, Fanny Bolis) against Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strain. Ethanolic extracts were prepared by freshly crushed leaves and evaluated for their antimicrobial activity against Escherichia coli ATCC® 25922™ strain using disc diffusion assay. The increase of the mean of the diameters of the inhibition zone was 58.4 % for cv. Kramer's Supreme, 29.2 % for cv. La Pace and cv. Mrs. Lyman Clarke, 22.5 % for cv. Fanny Bolis, 19.1% for cv. Benikarako, and 18 % for cv. C.M. Wilson compared to the control samples (96 % ethanol). Among the six plant extracts, C. japonica 'Kramer's Supreme' exhibited the highest inhibitory zones against the tested strain (the mean of the zone of inhibitions was 14.1 ±1.1 mm). The intermediate activity was presented by other cultivars studied. The findings reported herein give scientific credence to the traditional uses of these plants and suggest that extracts derived from the leaves of Camellia japonica and its cultivars merit further chemical study as natural antibiotics to identify the secondary metabolites. These results could provide a theoretical basis for making full use of Camellia japonica and its cultivars. Moreover, their antibacterial activities can play an important role in medicine, veterinary, food preservation, and other aspects. Mechanisms of antibacterial activities remain to be studied.

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Antibacterial efficacy of ethanolic extract of Camellia sinensis and Azadirachta indica leaves on methicillin-resistant Staphylococcus aureus and shiga-toxigenic Escherichia coli

Cited by 18 publications

References 31 publications

Blend of cassava starch and high-density polyethylene with green tea for food packaging

Blend of cassava starch and high-density polyethylene with green tea for food packaging

Antibacterial activity of Xenorhabdus and Photorhabdus isolated from entomopathogenic nematodes against antibiotic-resistant bacteria

Antimicrobial Efficacy of Ethanolic Extracts Obtained from Leaves of Camellia japonica L. Cultivars against Escherichia coli Strain

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