Antibacterial Effects of Lactic Acid Bacteria Cell Free Extract          on the Growth of Local Isolates of Listeria moncytogenes from some Food Sources

Alzubaidy, Zainab M.; Kakey, Shean I.; Ali, Jassim Fatehi

doi:10.33899/rjs.2014.86055

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Production of extracellular lipase aids in the degradation of lipids. The link of to these virulence factors raises public health concerns because they are notably bacterial pathogenic features (Alzubaidy ., 2013).…”

Section: Monocytogenes Et Al L Monocytogenesmentioning

confidence: 99%

Detection and characterization of Listeria monocytogenes from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Beshiru,

Uwhuba

2024

ijs

View full text Add to dashboard Cite

Outbreaks linked with foodborne pathogens and associated diseases occur regularly and pose substantial health problems to consumer safety globally. Listeriosis is a leading foodborne disease initiated by the consumption of foods contaminated by Listeria monocytogenes (L. monocytogenes ) This study evaluated the presence of . L. monocytogenes in various food samples. Locally fermented food samples (n = 80), which included Garri (n = 30), Kunu (n = 25), and Zobo (n = 25), were obtained from five open local markets in Ethiope West, Delta State, Nigeria. Food samples were screened for L. monocytogenes using standard cultural techniques, biochemical tests including the Analytic Profile Index (API) Listeria kit. The isolates were screened for antimicrobial resistance using the disc diffusion method, and breakpoints were interpreted based on the guidelines of the Clinical Laboratory Standard Institute. Gelatinase and lipase activities, including β-haemolysis, were determined using standard methods. L. monocytogenes was identified in 3 (3.8%) and 4 (5%) samples from Zobo and Kunu, respectively. The Garri samples were negative for L. monocytogenes, and only 7 of the 80 (8.8%) samples were positive for L. monocytogenes. One positive sample each was obtained in the Oghara and Ogharefe markets, two from the Ugbenu market, and three from the Mosogar market. No sample was positive L. monocytogenes. for in the Oghareki market. All the isolates were penicillin-resistant but susceptible to levofloxacin and tetracycline, whereas five were resistant to clindamycin. All isolates were resistant to at least two classes of antibiotics, and the multiple antibiotic resistance (MAR) index was ≥ 0.22. Gelatinase activity was observed in one isolate, β-haemolytic activity in four isolates, and lipase activity in five isolates. This study revealed the importance of proper hygiene practices in food production and continuous surveillance of antibiotic resistance and virulence determinants in food. There is a need to adhere to standard hygiene measures in food processing environments.

show abstract

Section: Monocytogenes Et Al L Monocytogenesmentioning

confidence: 99%

Detection and characterization of Listeria monocytogenes from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Beshiru,

Uwhuba

2024

ijs

View full text Add to dashboard Cite

show abstract

Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Cell-Free Extracts of Bifidobacterium Species Against Methicillin-Resistant Staphylococcus aureus in vitro

AL-Saadi¹

2016

AJBLS

View full text Add to dashboard Cite

The aim of this study was to examine if extract of bifidobacteria, a major species of the human colonic microflora participates in the barrier effect against enteropathogens by developing antimicrobial activity against virulent bacteria. Six human bifidobacteria strains were isolated from infant stools. They were characterized and identified through physiological, biochemical tests and API 20 A test system. The isolates belonged to the three species: B. breve, B.longum and B.infantis. The cell extracts of the isolates were examined for antimicrobial activity by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). For this purpose, the methicillin-resistant S. aureus (MRSA) was chosen as an indicator. MRSA treated with cell-free supernatants (CFS) from bifidobacteria were examined. All the Bifidobacterium isolates used have been identified as novel probiotics with a greater ability to survive at low pH and high concentrations of bile salt in vitro. 0.5 McFarland standard (10 8 CFU/ml) of a confirmed MRSA strain was challenged with the CFS strains by employing the tube dilution method and subculture on MRS agar assays. The cell-free supernatants of the 6 LAB strains exhibited MIC values between 50 µl/ml and 200 µl/ml. Only two CFS of bifidobacteria (b3 and b 4) had no MIC and MBC values with the concentrations under the current study. The b1, BL and BI strains showed highest antibacterial activity by MIC value with 100 conc. and by MBC value with 150 conc. Increased concentration levels of the cell-free extract (CFE) correlated with a decrease in MRSA viability. MRS broth medium (control) showed a high growth rate of MRSA without CFE.These results may provide a basis for alternative therapies for the treatment of MRSA superbug.

show abstract

Antibacterial Effects of Lactic Acid Bacteria Cell Free Extract on the Growth of Local Isolates of Listeria moncytogenes from some Food Sources

Cited by 2 publications

References 26 publications

Detection and characterization of <i>Listeria monocytogenes</i> from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Detection and characterization of <i>Listeria monocytogenes</i> from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Cell-Free Extracts of <i>Bifidobacterium</i> Species Against Methicillin-Resistant <i>Staphylococcus aureus in vitro</i>

Contact Info

Product

Resources

About

Antibacterial Effects of Lactic Acid Bacteria Cell Free Extract on the Growth of Local Isolates of Listeria moncytogenes from some Food Sources

Cited by 2 publications

References 26 publications

Detection and characterization of <i>Listeria monocytogenes</i> from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Detection and characterization of <i>Listeria monocytogenes</i> from locally processed fermented foods in Ethiope West, Delta State, Nigeria

Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Cell-Free Extracts of &lt;i&gt;Bifidobacterium&lt;/i&gt; Species Against Methicillin-Resistant &lt;i&gt;Staphylococcus aureus in vitro&lt;/i&gt;

Contact Info

Product

Resources

About

Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Cell-Free Extracts of <i>Bifidobacterium</i> Species Against Methicillin-Resistant <i>Staphylococcus aureus in vitro</i>