Antibacterial effectiveness of probiotic-based experimental mouthwash against cariogenic pathogen: An in vitro study

Abstract

Objectives:The aim of this study is to assess the antibacterial effectiveness of probiotic-experimental-based mouthwash (MW) against Streptococcus mutans in vitro.Materials and Methods:Antimicrobial screening of two active additives (probiotic-zamzam) was tested against S. mutans using disc diffusion method. A total of three MWs; (1) an experimental MW base formula, (2) an experimental MW base formula with the two active additives, and (3) commercial MW (hexitol), were evaluated against S. mutans by well diffusion method after 24 h and 72 h storage period. The survival profile of probiotic strain in the experimental MW was determined using colony counting method as well as the pH changes at three intervals. Statistical analysis was performed using one-way ANOVA and t-test to compare the inhibition zone diameter.Results:For active additives, probiotic strain exhibited higher mean inhibition zones values than zamzam water against S. mutans. Regarding the inhibition zones for the three tested MWs, the experimental MW showed significant increase in the inhibition zone after 72 h, while there was insignificant change with commercial MW. For probiotic count in MW, there was insignificant change in bacterial count after 24 h, and significant decrease after 15 days, followed by insignificant change after 30 days. For the pH values of the experimental MW, a statistically insignificant change was found after 24 h, significant decrease after 15 days and insignificant change after 30 days.Conclusions:The probiotic-zamzam experimental MW was effective in reducing S. mutans. Zamzam water could be considered as prebiotic ingredient. Therefore, the probiotic-zamzam MW has a potential therapeutic value.

Antibacterial and Remineralization Efficacy of Casein Phosphopeptide, Glycomacropeptide Nanocomplex, and Probiotics in Experimental Toothpastes: An In Vitro Comparative Study

“…This decline in the microhardness could be attributed to the nature of the L. rhamnosus bacteria which is a phosphate accumulating bacteria that tends to accumulate polyphosphates resulting in the decline of phosphate in the surrounding which can lead to undersaturated conditions relative to the hydroxyapatite which might lead to demineralization potentials in dental plaque and lesser degrees of remineralization in the conducted study. 41 Though the inhibitory effect of group (A3) toothpaste containing probiotic against S. mutans growth was in accordance with many studies, 42 43 the remineralization capacity of this group needs prolonged studies in addition to in vivo studies to observe the actual effect of this toothpaste in oral conditions. Regarding the nGMP group (A4), the highest microhardness remineralization results were reported for this group.…”

Oral probiotic and its delivery carriers to improve oral health: A review

“…A single-blinded in vivo trial and an in vitro study were conducted by Jothika et al [96] and Elgamily et al [97] to evaluate probiotic-mouthwash effectiveness in reducing S. mutans .…”

“…A single-blinded in vivo trial and an in vitro study were conducted by Jothika et al [96] and Elgamily et al [97] to evaluate probiotic-mouthwash effectiveness in reducing S. mutans. The in vivo study which recruited 52 subjects with a high level of S. mutans (>4 log 10 c.f.u.…”

Effect of Zamzam Water on Microhardness of Primary Tooth Enamel After Erosion Induced by Claritin Syrup

“…[ 23 ] Streptococcus mutans is considered one of the most cariogenic pathogens in the oral cavity, an experimental probiotic-Zamzam water mouthwash effectively reduces the S. mutans count in vitro . [ 24 ]…”