Antibacterial effect of silver nanoparticles in &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt;

Salomoni, R; Léo, Patrícia; Montemor, AF; Rinaldi, BG; Rodrigues, Maria Filomena de Andrade

doi:10.2147/nsa.s133415

Cited by 243 publications

(127 citation statements)

References 40 publications

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“…It is unlikely that the bacterial cell will develop "AgNP" resistance since silver displays multiple antimicrobial mechanisms against the bacteria 56 . The present study clearly depicts the antibacterial activity of biosynthesized AgNPs against both Gram-positive and Gram-negative bacteria; a considerable inhibitory pattern was observed similar to previous reports 57,58 .…”

Section: Morphology Of Agnps Containing Nanofilmssupporting

confidence: 90%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.activities of porous Ag nanofilm were investigated. We anticipate that the Ag nanofilm wound dressing with free radical scavenging capacity would enhance the wound healing process. Moreover, this study proposed a low-cost and facile method for preparation of Ag nanofilm as a potential wound dressing. Materials and MethodsMaterials. Methanol (CH 3 OH, 99.9%), silver nitrate (AgNO 3 ), nutrient agar, ferric chloride hexahydrate (FeCl 3 , 6H 2 O), iron (II) sulfate tetrahydrate (FeSO 4 .4H 2 O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco's Modified Eagle's Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa 38 and Staphylococcus aureus 39 bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.Extract preparation. T.polium is a wild-growing...

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Section: Morphology Of Agnps Containing Nanofilmssupporting

confidence: 90%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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“…Studies have addressed the sterilizing effect of Ag-NPs against numerous pathogens. Salomoni et al 14…”

Section: Discussionmentioning

confidence: 99%

Silver nanoparticles induce Egr‐1‐dependent psoriasin expression via the ERK and p38 pathways

et al. 2018

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Summary Background Silver nanoparticles (Ag‐NPs) can prevent bacterial infection and improve cutaneous wound healing owing to their antimicrobial activity. However, the mechanism of their antimicrobial activity is poorly understood. Aim To determine the mechanistic relationship between Ag‐NP treatment and expression of psoriasin. Methods Human epidermal keratinocytes, neonatal (HEKn) were used. Psoriasin mRNA expression was measured by reverse transcription PCR and real‐time PCR. Western blotting was performed to verify expression of early growth response‐1 (Egr‐1) and psoriasin, and phosphorylation of mitogen‐activated protein kinase (MAPK). Psoriasin promoter activity by Egr‐1 was detected by a luciferase assay. Results Treatment of HEKn with Ag‐NPs induced psoriasin mRNA and protein expression. Upregulation of psoriasin promoter activity was also observed in the luciferase assay. Ag‐NPs increased Egr‐1 expression, promoter activity and nuclear translocation in HEKn. Psoriasin luciferase activity was increased in HEKn transfected with Egr‐1 pcDNA 3.1. Ag‐NPs activated MAPK pathways including the extracellular signal‐regulated kinase (ERK), p38, and c‐Jun‐N‐terminal kinase (JNK) pathways. The upregulation of Egr‐1 expression by Ag‐NP stimulation was inhibited by ERK and p38 inhibitors, but not by a JNK inhibitor. Psoriasin expression was reduced in Egr‐1 small interfering RNA‐transfected HEKn. Conclusions Ag‐NP treatment induces upregulation of psoriasin expression through Egr‐1 expression. We suggest that the ERK and p38 pathways are involved in Egr‐1‐dependent psoriasin expression.

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“…An amount of 10 μl of microbial suspension was added to 10 ml of normal saline, to match McFarland's standard (0.5) and used to prepare seeded nutrient agar plates. The disc diffusion method was used, wherein filter paper discs soaked with the test samples, namely formulation gel, silver nitrate and extract, were inoculated on the seeded agar plates and were incubated for 24 h . The average diameter of the inhibition zones surrounding the discs was measured in millimetres and the statistical significance was calculated using GraphPad Prism 7 software.…”

Section: Methodsmentioning

confidence: 99%

Pongamia pinnata seed extract‐mediated green synthesis of silver nanoparticles: Preparation, formulation and evaluation of bactericidal and wound healing potential

Paul

Londhe

2018

Applied Organom Chemis

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Pongamia pinnata – a plant used since olden times in Ayurvedic treatment – is reported to have diverse functions including antibacterial, antidiabetic, antineurodegenerative, antiepileptic, antiulcer, etc. In this study, our objective was to prepare silver nanoparticles (AgNPs) by green synthesis mediated by methanolic seed extract of P. pinnata and to determine their antimicrobial and antioxidant potential and wound healing activity. AgNPs were characterized for particle size and shape and for antioxidant potential. Further, the AgNPs were incorporated in a gel. The wound healing activity was investigated using an excision wound healing model in Wistar rats. The AgNP‐loaded gel was applied topically to the wounded rats daily for 30 days. The wound contraction was calculated and histopathological studies of the healed tissues were conducted. Karanjin content of the extract was found to be 349 ± 2.16 mg g−1. Formation of AgNPs was confirmed using transmission and scanning electron microscopies and X‐ray diffraction. AgNPs showed good antioxidant potential and were active against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. Significant wound healing activity (p < 0.05) was shown by the AgNP gel as compared to 5% Betadine ointment. Thus, the prepared AgNPs have antimicrobial and wound healing effects that may be useful in treatment of topical infections especially in wounds.

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Antibacterial effect of silver nanoparticles in <em>Pseudomonas aeruginosa</em>

Cited by 243 publications

References 40 publications

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Silver nanoparticles induce Egr‐1‐dependent psoriasin expression via the ERK and p38 pathways

Pongamia pinnata seed extract‐mediated green synthesis of silver nanoparticles: Preparation, formulation and evaluation of bactericidal and wound healing potential

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