Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains

Anitua, Eduardo; Alonso, Rodrigo; Girbau, Cecilia; Aguirre, José Antonio Cadiñanos; Muruzábal, Francisco; Orive, Gorka

doi:10.1111/j.1365-2230.2011.04303.x

Cited by 128 publications

(120 citation statements)

References 23 publications

(22 reference statements)

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“…Although leukocytes have been proposed as an additional source for cytokines, microbicidal proteins and myeloperoxidase activity (Hampton et al, 1996), the few available published data do not support the increased microbicidal activity of PRP due to the leukocyte component (Bielecki et al, 2007;Moojen et al, 2008;Anitua et al, 2012). Therefore in this study we used the Anitua's protocol (Anitua et al, 2008) that allows the production of leukocyte-poor PRP.…”

Section: Discussionmentioning

confidence: 99%

“…Despite extensive use in clinical procedures, only few studies have investigated PRP's microbicidal activity (Bielecki et al, 2007;Moojen et al, 2008;Anitua et al, 2012;Burnouf et al, 2013;Drago et al, 2013). Mariani et al (2015) tested the effect of PRP against Escheri chia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Enterococcus faecalis, as species potentially involved in bone, soft tissue and wound infections (Zalavras et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Antiviral Resistance of Splenocytes in Aged Mice

Zaczyńska

Artym

Kocięba

et al. 2017

Polish Journal of Microbiology

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A b s t r a c tAntimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.K e y w o r d s: antimicrobial activity, oral infection, platelet-rich plasma

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiviral Resistance of Splenocytes in Aged Mice

Zaczyńska

Artym

Kocięba

et al. 2017

Polish Journal of Microbiology

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“…Recent data suggest that the pool of molecules playing a role in PRGF eyedrops is extensive, including trophic factors [16], anti-inflammatory agents [17] and bacteriostatic/bactericide molecules [18,19]. It has been reported that PRGF significantly enhances the proliferation and migration of both keratocytes and conjunctival fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Autologous Plasma Rich in Growth Factors Eyedrops in Refractory Cases of Ocular Surface Disorders

et al. 2015

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Purpose: Preliminary information about the safety and efficacy of plasma rich in growth factors (PRGF) eyedrops in the treatment of refractory cases of diverse ocular surface disorders (OSDs) is presented here. Material and Methods: This retrospective cohort study included cases with OSDs refractory to previous treatment with conventional treatments or autologous serum or cyclosporine, and treated with PRGF eyedrops. The signs and symptoms of ocular surface disorders [using the ocular surface disease index (OSDI), best-corrected visual acuity (BCVA), visual analog scale (VAS) frequency and VAS severity] were evaluated before and after treatment with PRGF. A safety assessment was also performed reporting all adverse events or complications. Results: Forty-one patients with a total of 80 treated eyes were evaluated. Statistically significant reductions in the OSDI scale (39.27%), VAS frequency (38.9%) and VAS severity (40.3%), and a significant improvement in BCVA (54.86%) were all observed (p < 0.05). The results were stratified according to the identified potential effect modifiers. There were only two adverse events (eye redness and eyelid inflammation), which were reported as mild and resolved in a few days. Conclusions: PRGF eyedrops could be a safe and effective treatment option for refractory cases of OSDs. When treating patients the possible influence on the results of some clinical variables must be taken into account.

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“…Recent data suggest that the pool of molecules playing a role in PRGF eye drops is large, including trophic factors [21], anti-inflammatory agents [22,] and bacteriostatic/bactericidal molecules [23,24]. In addition, it has been reported that PRGF significantly enhances proliferation and migration of both keratocytes and conjunctival fibroblasts, while it prevents and inhibits TGFβ 1 -induced myofibroblast differentiation [16].…”

Section: Discussionmentioning

confidence: 99%

Safety and Efficacy of Autologous Plasma Rich in Growth Factors Eye Drops for the Treatment of Evaporative Dry Eye

Merayo‐Lloves

Sánchez-Ávila²,

Riestra³

et al. 2016

Ophthalmic Res

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Purpose: To provide preliminary information about the safety and efficacy of plasma rich in growth factors (PRGF) in the treatment of evaporative dry eye (EDE) and analyze the influence of certain variables on treatment outcomes. Methods: This retrospective study included patients with EDE treated between January 2011 and December 2013. Outcome measures including signs and symptoms of the disease were evaluated before and after treatment. The influence of some of the patient's clinical variables on results was assessed. Safety assessment was also performed. Results: Eighty-three patients with a total of 156 eyes were evaluated. Statistically significant reductions in the Ocular Surface Disease Index (38.2%), best-corrected visual acuity (27.4%), Visual Analogue Scale scores for frequency (32%) and severity (34%), and a significant improvement in the Schirmer test scores (177.5%) were observed (p < 0.05). The results were stratified according to the identified potential effect modifiers. Only four adverse events were reported. All of them were mild and resolved within a few days. Conclusions: Results suggest that PRGF eye drops could be a safe and effective treatment in reducing EDE signs and symptoms. When treating patients, the possible influence of some clinical variables must be taken into account.

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Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains

Abstract: This is the first time different formulations of this product have been evaluated, and the results suggest that PRGF-Endoret could be used in the fight against postoperative and wound infections.

Cited by 128 publications

References 23 publications

Antiviral Resistance of Splenocytes in Aged Mice

Antiviral Resistance of Splenocytes in Aged Mice

Autologous Plasma Rich in Growth Factors Eyedrops in Refractory Cases of Ocular Surface Disorders

Safety and Efficacy of Autologous Plasma Rich in Growth Factors Eye Drops for the Treatment of Evaporative Dry Eye

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