Antibacterial Effect of Ethanolic Extract of Allium sativum on Biofilm Forming Staphylococcus aureus which Cause Folliculitis

Bayati, Shatha Al

doi:10.20546/ijcmas.2018.701.229

Cited by 6 publications

(7 citation statements)

References 25 publications

(17 reference statements)

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“…We prepared fresh raw garlic extract to be used, as its antimicrobial activity was reported to be superior to its isolated compounds (Venâncio et al, 2017). In agreement with others (Bayati, 2018), FGE showed potent antibacterial activity that was concentration and strain-dependent. Among the five test strains, the highest MIC value was recorded with K. pneumoniae .…”

Section: Discussionsupporting

confidence: 92%

“…Other investigators (Mohsenipour, 2015, Pakdel et al, 2017) didn’t record prominent antibacterial activity for either the aqueous or alcoholic extract of garlic by the disk diffusion methods. However, many factors could affect the results; such as the plant origin, period of the year, extraction methods, microbial test strains (Bayati, 2018, Pakdel et al, 2017), testing methodology (agar well, filter paper, macro-broth dilution or micro-broth dilution) and storage conditions. The antibacterial activity of fresh garlic was found to be superior to garlic powder, garlic oil and butylated hydroxyanisole (Sallam et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm plays a vital role in pathogenicity, virulence and antimicrobial resistance of microbial pathogens (Bayati, 2018, Tewari et al, 2018). We found that sub-MICs of FGE showed an evident antibiofilm activity in a concentration-dependent fashion.…”

Section: Discussionmentioning

confidence: 99%

“…, Shigella spp. and S. aureus (Abiy and Berhe, 2016, Bayati, 2018). Even fatal diseases such as anthrax and tuberculosis were found to be sensitive to garlic components (Abiy and Berhe, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects

Farrag

Hosny

Hawas

et al. 2019

Saudi Pharmaceutical Journal

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BackgroundLife-threatening central venous catheter-related infections are primarily initiated by biofilm formation on the catheter surface. Antibiotic lock therapy is recommended for eradicating intraluminal biofilm. In the era of antibiotic resistance, antibiotics of natural origins provide an effective and cheap option for combating resistant strains. Garlic especially stole the spotlight because of its impressive antimicrobial effectiveness against such superbugs.AimIs to estimate the potential use of fresh garlic extract (FGE) as a lock agent against multi-drug resistant (MDR) bacteria.MethodsThe agar well diffusion and broth microdilution techniques were employed to test the antimicrobial activities of FGE against five MDR strains; E. coli, Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae), Serratia marscens (S. marscens) and Methicillin-resistant Staphylococcus aureus (MRSA). Then the protective and therapeutic efficiencies of FGE against bacterial biofilms were in-vitro evaluated; at concentrations of 100, 75, 50 and 25%; in tissue culture plate (TCP) and on the polyurethane (PU) sheets using the crystal violet (CV) assay and colony-forming unit (CFU), respectively. Scanning electron microscopy (SEM) was also used to confirm eradication of biofilms on PU sheets. Finally, systemic and deep tissue infections by P. aeruginosa and MRSA were induced in mice that were then treated by FGE at either 100 or 200 mg/kg for seven days. Where the antibacterial activity was assessed by tissue and blood culturing at the end of the treatment period. Biochemical, hematological and histological parameters were also investigated.ResultsFGE exhibited potent in-vitro and in-vivo antibacterial and antibiofilm activities against MDR strains. It not only didn’t exhibit toxicological effects at the hematological and the histological levels but also provided protective effects as demonstrated by the significant drop in the biochemical parameters.ConclusionFGE has the potential to be used as a prophylactic and/or therapeutic lock agent against biofilm-associated infections caused by MDR bacteria.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects

Farrag

Hosny

Hawas

et al. 2019

Saudi Pharmaceutical Journal

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“…Resultantly, S. aureus was found to be inhibited up to 52.8% with garlic extract (Mohsenipour & Hassanshahian, ). The study of Al Bayati () further confirmed the inhibition of biofilm forming S. aureus with ethanolic extract of garlic. Our study results are supported by the study of Sandasi, Leonard, and Viljoen () who proved the inhibitory effects of A. sativum extracts against biofilm formation of different microbes and found that effect of alcoholic extracts was higher than that of aqueous extract of garlic.…”

Section: Resultsmentioning

confidence: 83%

Characterization and antimicrobial potential of bioactive components of sonicated extract from garlic ( Allium sativum ) against foodborne pathogens

Liaqat

Zahoor

Randhawa

et al. 2019

J Food Process Preserv

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Garlic (Allium sativum) belongs to Liliaceae family. Plants belonging to this family are well known for their sulfur compounds besides phenolic substances. The current study was planned to explore the potential of sonication treatment combined with various solvents for extracting bioactive components (phenolics and allicin) of two local garlic varieties that is garlic white and garlic pink and to elucidate the antimicrobial potential of extracts against selected pathogens. Total phenolic contents in various extracts ranged 57.35 ± 4.77–86.18 ± 1.53mg GAE/100 g. Allicin as characterized through high‐performance liquid chromatographic system was found in the range of 62.67 ± 13.49–425.6 ± 3.04 ppm. The minimum inhibitory concentration of garlic extracts as calculated by the broth dilution method ranged 25–133 mg/ml and biofilm formation was 45–82%. On an overall basis, garlic pink indicated higher bioactive components, antimicrobial potential against foodborne pathogens, and biofilm formation inhibition. Regarding solvents extracts with 50% methanol, as extraction solvent, indicated better results trailed by 50% ethanol and water. Practical applications Garlic extract is rich in bioactive moieties especially Allicin, other organosulfur and phenolics, having antioxidant and antimicrobial potential which can exert beneficial effects on human health. Antimicrobial potential of garlic extract can be utilized for killing foodborne pathogens and thus achieving food safety. Its use in meat, meat products, and fish can significantly reduce the pathogens associated with these food items. Moreover, both antioxidant and antimicrobial properties offer bio‐preservation effect to these products and extend the shelf life. Biofilm formation inhibition properties of garlic extract can be utilized in food processing and preservation as well in food processing equipment. Further, garlic extracts can be used for preparation of nanoparticles with enhanced efficiency.

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Antibacterial and Antibiofilm Properties of Medicinal Plant Extracts against Multi Drug Resistant Staphylococcus Species and Non Fermenter Bacteria

Mehrishi¹,

Agarwal²,

Broor³

et al. 2020

J. Pure Appl. Microbiol.

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Antimicrobial resistance to the pathogenic microorganism has been characterized as a public health emergency both in the community and in hospitals. That is why; we need to find alternatives, which could be used as antibacterial agents. Therefore aim of this study is to determine the antibacterial and antibiofilm properties of 4 plant extracts Clove (Syzygium aromaticum), Tea (Camellia sinensis), Garlic (Allium sativum), coriander (Coriandrum sativum).Antibacterial properties of plant extracts at different concentrations (50, 25, 12.5, 6.25 mg/mL) were tested against Multi Drug Resistance biofilm producing Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus epidermidis and Staphylococcus saprophyticus using the agar well diffusion method.Minimum Bactericidal Concentration (MBC) and antibiofilm properties of the plant extracts were determined using the tube dilution method and modified crystal violet assay, respectively. Total of 180 clinical isolates were screened for their MDR Pattern. Out of these, 72 were MDR isolates. These MDR isolates were categorized into weak, moderate and strong biofilm producers. Fourteen, Forty nine and nine were weak, moderate and strong biofilm producers, respectively. Out of the 4 plant extracts, Syzygium aromaticum and Camellia sinensis were found to be more effective with maximum zone of inhibition (20-25 mm), MBC 6.25 mg/ml and biofilm reduction of more than 50% compared to Allium sativum and Coriandrum sativum. All medicinal plant extracts were effective at different concentrations against the biofilm producing MDR isolates but Syzygium aromaticum and Camellia sinensis showed maximum antibacterial and antibiofilm activity.

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Antibacterial Effect of Ethanolic Extract of Allium sativum on Biofilm Forming Staphylococcus aureus which Cause Folliculitis

Cited by 6 publications

References 25 publications

Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects

Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects

Characterization and antimicrobial potential of bioactive components of sonicated extract from garlic ( Allium sativum ) against foodborne pathogens

Antibacterial and Antibiofilm Properties of Medicinal Plant Extracts against Multi Drug Resistant Staphylococcus Species and Non Fermenter Bacteria

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