Antibacterial effect of composite resins containing quaternary ammonium polyethyleneimine nanoparticles

Yudovin-Farber, Ira; Beyth, Nurit; Weiss, Ervin I.; Domb, Abraham J.

doi:10.1007/s11051-009-9628-8

Cited by 114 publications

(95 citation statements)

References 26 publications

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“…Of note, QA-PEI has been studied as antibacterial additive incorporated in clinically used restorative composite resins [19]. We have also shown that octyl alkylated QA-PEI based nanoparticles embedded in a restorative composite resin at 1% w/w demonstrated strong antibacterial effect against S. mutans which lasted for at least 3 months [20]. Moreover, incorporation of QA-PEI based nanoparticles in dental composite resin at 1% w/w was biocompatible [21].…”

Section: Introductionmentioning

confidence: 76%

“…According to the first method, QA-PEI nanoparticles were synthesized by crosslinking of polyethyleneimine with glutaraldehyde followed by reductive amination with octanal and further Nmethylation with methyl iodide as illustrated in Scheme 1(a). The second method is based on crosslinking with dibromopentane followed by N-alkylation with octyl halide and further N-methylation with methyl iodide resulting in nanoparticles of spherical shape [20] (Scheme 1(b)). …”

Section: Resultsmentioning

confidence: 99%

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Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Yudovin-Farber

Golenser

Beyth

et al. 2010

Journal of Nanomaterials

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Quaternary ammonium polyethyleneimine-(QA-PEI-) based nanoparticles were synthesized using two synthetic methods, reductive amination and N-alkylation. According to the first method, QA-PEI nanoparticles were synthesized by cross-linking with glutaraldehyde followed by reductive amination with octanal and further N-methylation with methyl iodide. The second method is based on crosslinking with dialkyl halide followed by N-alkylation with octyl halide and further N-methylation with methyl iodide. QA-PEI nanoparticles completely inhibited bacterial growth (>10 6 bacteria), including both Gram-positive, that is, Staphylococcus aureus at 80 μg/mL, and Gram-negative, that is, Escherichia coli at 320 μg/mL. Activity analysis revealed that the degree of alkylation and N-methylation of the QA-PEI nanoparticles plays a significant role in antibacterial activity of the reagent. The most potent compound was octyl alkylated QA-PEI alkylated at 1 : 1 mole ratio (primary amine of PEI monomer units/alkylating agent). Also, cytotoxicity studies on MAT-LyLu and MBT cell lines were performed with QA-PEI nanoparticles. These findings confirm previous reports that polycations bearing quaternary ammonium moieties inhibit bacterial growth in vitro and have a potential use as additives in medical devices which need antibacterial properties.

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Section: Introductionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Yudovin-Farber

Golenser

Beyth

et al. 2010

Journal of Nanomaterials

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“…We thought that antibacterial modification of the dental resin composite is an advantageous approach for preparing materials that are continuously challenged physically in a harsh environment for many years. The incorporation of these QPEI nanoparticles in resin composite rendered a long-lasing antimicrobial effect against a wide range of bacteria with no measured influence on biocompatibility and without leaching (17)(18)(19). Furthermore, we have found that QPEI nanoparticle quarternization with octyl groups yields optimal antibacterial properties for dental composite restorative material, i.e.…”

mentioning

confidence: 88%

“…For this purpose and to overcome the disadvantages of materials that release antibacterial agents (13)(14)(15), we prepared more than 50 derivatives of polycationic particles, starting from different polyamines and alkyl halides that had 4 to 16 methylene groups, using different methods of synthesis. Although some of the tested nanoparticles were found to be effective in inhibiting bacterial growth, octyl alkylated QPEI nanoparticles incorporated into dental resin composite at 1% wt∕wt showed a superior antibacterial effect (16,17). These nanoparticles were mixed into prepolymerized commercially available dental resin composites, modifying the restoration material.…”

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confidence: 99%

Polyethyleneimine nanoparticles incorporated into resin composite cause cell death and trigger biofilm stress in vivo

Beyth

Yudovin-Farber²,

Davidi³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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170

126

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Incorporation of cross-linked quaternary ammonium polyethylenimine (QPEI) nanoparticles in dental resin composite has a longlasting and wide antimicrobial effect with no measured impact on biocompatibility in vitro. We hypothesized that QPEI nanoparticles incorporated into a resin composite have a potent antibacterial effect in vivo and that this stress condition triggers a suicide module in the bacterial biofilm. Ten volunteers wore a removable acrylic appliance, in which two control resin composite specimens and two resin composite specimens incorporating 1% wt∕wt QPEI nanoparticles were inserted to allow the buildup of intraoral biofilms. After 4 h, the specimens were removed and tested for bacterial vitality and biofilm thickness, using confocal laser scanning microscopy. The vitality rate in specimens incorporating QPEI was reduced by >50% (p < 0.00001), whereas biofilm thickness was increased (p < 0.05). The ability of the biofilm supernatant to restore bacterial death was tested in vitro. The in vitro tests showed a 70% decrease in viable bacteria (p < 0.05). Biofilm morphological differences were also observed in the scanning electron microscope micrographs of the resin composite versus the resin composite incorporating QPEI. These results strongly suggest that QPEI nanoparticles incorporated at a low concentration in resin composite exert a significant in vivo antibiofilm activity and exhibit a potent broad spectrum antibacterial activity against salivary bacteria.

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“…As shown in Figure 4, MIC against E. coli of poly(BVPB-co-NVP) with a charge density of 2.83 meq/g was 25 ppm, while poly(BVPC-co-NVP) exhibited a MIC value of 50 ppm even when the charge density was as high as 3.16 meq/g. Previous research has revealed that the effect of counter anions on the antibacterial activities of QAS is correlated with their steric hindrance and the solubility of the biocides [30,31]. While in the case of polycations with chloride and bromide as the counter anions, both the anions are halides and can dissociate freely in water.…”

Section: Evaluation Of Antimicrobial Activitymentioning

confidence: 99%

Antibacterial/Antiviral Property and Mechanism of Dual-Functional Quaternized Pyridinium-type Copolymer

Xue

Xiao

2015

Polymers

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Due to the massive outbreaks of pathogen-caused diseases and the increase of drug-resistant pathogens, there is a particular interest in the development of novel disinfection agents with broad-spectrum antipathogenic activity.In the present study, water-soluble pyridinium-type polyvinylpyrrolidones with different counter anions were prepared. Structural characterization was conducted via 13 C-1 H heteronuclear single quantum coherence spectroscopy, static light scattering, UV spectrometry and apparent charge density. The influence of counter anion and polymer compositions on the antibacterial activity was studied against Staphylococcus aureus and Escherichia coli. Atomic force microscopy (AFM) was applied for tracking the morphological alterations in bacterial cells induced by prepared polycations. It was found that the exposure of bacteria to the polycations resulted in the destruction of cell membranes and the leakage of cytoplasm. The antiviral activity of pyridinium-type polycations against enveloped influenza virus was evaluated via a plaque assay. The action mode against enveloped virus was depicted to rationalize the antiviral mechanism.

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Antibacterial effect of composite resins containing quaternary ammonium polyethyleneimine nanoparticles

Cited by 114 publications

References 26 publications

Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Polyethyleneimine nanoparticles incorporated into resin composite cause cell death and trigger biofilm stress in vivo

Antibacterial/Antiviral Property and Mechanism of Dual-Functional Quaternized Pyridinium-type Copolymer

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