Antibacterial composite membranes of polycaprolactone/gelatin loaded with zinc oxide nanoparticles for guided tissue regeneration

Prado-Prone, Gina; Silva-Bermúdez, Phaedra; Bazzar, Masoomeh; Focarete, Maria Letizia; Rodil, Sandra E.; Vidal-Gutiérrez, Ximena; García-Macedo, Jorge A.; García-Pérez, Victor I; Velasquillo, Cristina; Almaguer‐Flores, Argelia

doi:10.1088/1748-605x/ab70ef

Cited by 32 publications

(27 citation statements)

References 74 publications

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“…This causes weak points in the electrospun mat. The results are in agreement with that obtained by Augustine et al 32 and also the results of Prado-Prone et al 43 The average Young’s modulus, percentage elongation at break, and tensile strength of the mats were given in Table 5.…”

Section: Resultssupporting

confidence: 91%

Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

Ekram

El‐Hady

El-Kady

et al. 2021

Journal of Bioactive and Compatible Polymers

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In this study, we have investigated the effect of adding zinc chloride (ZnCl2) on polycaprolactone (PCL) before and after electrospinning. The rheological properties and conductivity of ZnCl2/PCL solutions were measured prior to the electrospinning process. The resultant electrospun mats were characterized by SEM, contact angle, FTIR, XRD, mechanical properties, as well as its antibacterial and stem cell proliferation assessment were tested. It was found that the fibers became finer by increasing the zinc salt content. Moreover, stability increased slightly up to 5% Zn-PCL and also the hydrophilicity has been enhanced by 52%. By adding ZnCl2, the degradation rate and mechanical properties were significantly increased. Also, the resultant mats have shown antibacterial properties against S. aureus than E. coli. From the stem cells proliferation study, it can be observed that by increasing ZnCl2, the stem cells proliferation was significantly increased. Grooved multichannel nerve conduits were successfully fabricated by rolling the electrospun mats produced on corn husks which has shown better cell alignment and attachment. Hence, adding zinc chloride is a facile biocompatible enhancement to polycaprolactone nanofibers to be used in nerve regeneration.

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Section: Resultssupporting

confidence: 91%

Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

Ekram

El‐Hady

El-Kady

et al. 2021

Journal of Bioactive and Compatible Polymers

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“…However, several studies reported that doping ZnO-NPs would increase the water contact angle and reduce the material's hydrophilicity, impairing osteoblast adhesion. 96,142 Osteogenic Properties of ZnO-NP-Modified Implants…”

Section: Dovepressmentioning

confidence: 99%

<p>Enhancing ZnO-NP Antibacterial and Osteogenesis Properties in Orthopedic Applications: A Review</p>

Li¹,

Yang²,

Qing³

et al. 2020

IJN

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Prosthesis-associated infections and aseptic loosening are major causes of implant failure. There is an urgent need to improve the antibacterial ability and osseointegration of orthopedic implants. Zinc oxide nanoparticles (ZnO-NPs) are a common type of zinccontaining metal oxide nanoparticles that have been widely studied in many fields, such as food packaging, pollution treatment, and biomedicine. The ZnO-NPs have low toxicity and good biological functions, as well as antibacterial, anticancer, and osteogenic capabilities. Furthermore, ZnO-NPs can be easily obtained through various methods. Among them, green preparation methods can improve the bioactivity of ZnO-NPs and strengthen their potential application in the biological field. This review discusses the antibacterial abilities of ZnO-NPs, including mechanisms and influencing factors. The toxicity and shortcomings of anticancer applications are summarized. Furthermore, osteogenic mechanisms and synergy with other materials are introduced. Green preparation methods are also briefly reviewed.

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“…While the GTR technique widely uses membrane materials as a barrier in periodontal flap surgery, it still has some limitations, such as poor osteogenic and antimicrobial properties (Liang et al, 2020). To endow these biodegradable membrane with excellent osteogenic and antimicrobial activities, there are several emerging approaches (Naahidi et al, 2017;Prado-Prone et al, 2020;Shkodenko et al, 2020), among which MgO nanoparticlesincorporated PCL/gelatin nanocellulose membranes fabricated by coaxial electrospinning might be very promising platforms for periodontal tissue regeneration. Our results indicated that Coaxial-MgO had good biocompatibility and affinity to cells due to its gelatin shell, and incorporation of MgO nanoparticles could enhance the mechanical property of membrane.…”

Section: Discussionmentioning

confidence: 99%

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

Peng

Ren

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Electrospinning technique has attracted considerable attention in fabrication of cellulose nanofibrils or nanocellulose membranes, in which polycaprolactone (PCL) could be used as a promising precursor to prepare various cellulose nanofibril membranes for periodontal tissue regeneration. Conventional bio-membranes and cellulose films used in guided tissue regeneration (GTR) can prevent the downgrowth of epithelial cells, fibroblasts, and connective tissue in the area of tooth root but have limitations related to osteogenic and antimicrobial properties. Cellulose nanofibrils can be used as an ideal drug delivery material to encapsulate and carry some drugs. In this study, magnesium oxide (MgO) nanoparticles-incorporated PCL/gelatin core-shell nanocellulose periodontal membranes were fabricated using coaxial electrospinning technique, which was termed as Coaxial-MgO. The membranes using single-nozzle electrospinning technique, namely Blending-MgO and Blending-Blank, were used as control. The morphology and physicochemical property of these nanocellulose membranes were characterized by scanning electron microscopy (SEM), energy-dispersive spectrum of X-ray (EDS), transmission electron microscopy (TEM), contact angle, and thermogravimetric analysis (TGA). The results showed that the incorporation of MgO nanoparticles barely affected the morphology and mechanical property of nanocellulose membranes. Coaxial-MgO with core-shell fiber structure had better hydrophilic property and sustainable release of magnesium ion (Mg2+). CCK-8 cell proliferation and EdU staining demonstrated that Coaxial-MgO membranes showed better human periodontal ligament stem cells (hPDLSCs) proliferation rates compared with the other group due to its gelatin shell with great biocompatibility and hydrophilicity. SEM and immunofluorescence assay results illustrated that the Coaxial-MgO scaffold significantly enhanced hPDLSCs adhesion. In vitro osteogenic and antibacterial properties showed that Coaxial-MgO membrane enhanced alkaline phosphatase (ALP) activity, formation of mineralized nodules, osteogenic-related genes [ALP, collagen type 1 (COL1), runt-related transcription factor 2 (Runx2)], and high antibacterial properties toward Escherichia coli (E. coli) and Actinobacillus actinomycetemcomitans (A. a) when compared with controls. Our findings suggested that MgO nanoparticles-incorporated coaxial electrospinning PCL-derived nanocellulose periodontal membranes might have great prospects for periodontal tissue regeneration.

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Antibacterial composite membranes of polycaprolactone/gelatin loaded with zinc oxide nanoparticles for guided tissue regeneration

Abstract: Antibacterial composite membranes of PCL/gelatin loaded with ZnO nanoparticles for guided tissue regeneration.

Cited by 32 publications

References 74 publications

Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

<p>Enhancing ZnO-NP Antibacterial and Osteogenesis Properties in Orthopedic Applications: A Review</p>

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

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