Antibacterial, anticoagulant and cytotoxic evaluation of biocompatible nanocomposite of chitosan loaded green synthesized bioinspired silver nanoparticles

Asghar, Muhammad Arif; Yousuf, Rabia Ismail; Shoaib, Muhammad Harris; Asghar, Muhammad Asif

doi:10.1016/j.ijbiomac.2020.05.197

Cited by 66 publications

(43 citation statements)

References 49 publications

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“…In our previous work, IC 50 value of CS-AgNPs was reported as 125 µg/mL. 23 In the current study, IC 50 values of CS-AgNPs were slightly decreased after conjugation with different antibiotics. However, a considerable reduction in MICs and FICs (Tables 2 and 3, Figure 7), suggested the dose reduction Table 4.…”

Section: Cs-agnpssupporting

confidence: 59%

“…Green synthesis of AgNPs using Syzygium aromaticum ethanolic extract (SAEE) and functionalized with chitosan (CS-AgNPs) was described in our previous study. 23 Briefly, AgNPs were synthesized by adding SAEE (10 mg/mL) dropwise to the AgNO 3 (1 mmol/L) solution in the ratio of 9:1. At room temperature, the mixture was continuously stirred and left in a darkroom for 24 h to prevent photodegradation of silver ions.…”

Section: Antibiotics Loading On Synthesized Chitosan-based Silver Nanmentioning

confidence: 99%

“…The percentage viability of cells was determined using MTT (3 (4,5 dimethyl thiazol 2) 2.5 diphenyltetrazolium bromide) assay, as reported in previous studies. 21,23 For the determination of IC 50, all antibiotics in the concentrations range of 0.016-1024 μg/mL were used, whereas CS-AgNPs conjugated with AMX, CEF, and LVX, were applied in the concentrations according to their respective FICs (defined in Table 3). Briefly, prior to the incubation for 24 h, each test solution was added separately in an adherent culture medium.…”

Section: Toxicity Studiesmentioning

confidence: 99%

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<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

Asghar

Yousuf

Shoaib

et al. 2020

IJN

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The present study reports chitosan functionalized green synthesized CS-AgNPs, conjugated with amoxicillin (AMX), cefixime (CEF), and levofloxacin (LVX) for safe and enhanced antibacterial activity. Methods: The CS-AgNPs and conjugates CS-AgNPs+AMX CS-AgNPs+CEF, and CS-AgNPs+LVX were characterized by UV-Vis, FTIR, SEM, TEM, EDX spectroscopy. The size distribution and zeta potential were measured using the dynamic light scattering (DLS) technique. The interaction between CS-AgNPs and antibiotic molecules was also investigated using UV-Vis spectroscopy at the concentrations of 5, 50, 500, and 5000 µM for each antibiotic. Antibacterial activity and synergism were assessed by the Fractional Inhibitory Concentration (FIC) index. The mechanism for synergistic activity was investigated by the detection of hydroxyl species based on the chemiluminescence of luminol. The biocompatibility index (BI) was calculated from IC 50 using the HeLa cell line. In vivo toxicity and tissue distribution of silver ions were evaluated on Sprague Dawley rats. Physical interactions of antibiotics and significant (P<0.05) antibacterial activity were observed after loading on CS-AgNPs surfaces. Results: The spherical shape nanocomposites of CS-AgNPs with different antibiotics were prepared with mean size ranges of 80-120 nm. IC 50 of antibiotics-conjugated CS-AgNPs decreased compared to CS-AgNPs. The biocompatibility (BI) index showed that antibioticsconjugated CS-AgNPs have high antibacterial potential and low toxicity. Highly significant (P<0.005) increase in the generation of hydroxyl species indicated the radical scavenging mechanism for synergistic activity of CS-AgNPs after combined with different antibiotics. Biochemical analysis and histopathological examinations confirmed low toxicity with minor hepatotoxicity at higher doses. After oral administration, extensive distribution of Ag ion was observed in spleen and liver. Conclusion: The study demonstrates positive attributes of antibiotics-conjugated CS-AgNPs, as a promising antibacterial agent with low toxicity.

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Section: Cs-agnpssupporting

confidence: 59%

Section: Antibiotics Loading On Synthesized Chitosan-based Silver Nanmentioning

confidence: 99%

Section: Toxicity Studiesmentioning

confidence: 99%

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<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

Asghar

Yousuf

Shoaib

et al. 2020

IJN

Self Cite

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“…Sources used to create the illustration. [157,163,164,167,168] Route Synthesis method a) Size and shape Microorganisms and antimicrobial activity (MIC/MBC/D) b) Refs. by the characteristic plasmon band at 415 nm (UV-vis absorption spectra) and the FCC lattice (XRD diffractogram).…”

Section: Synthesis Methodsmentioning

confidence: 99%

“…[85,157,163] Chitosan can react with both the cell wall and the external membrane of gram-positive and gram-negative bacteria. [163] The positively charged amino groups in CsNPs interact with negatively charged components in bacterial cell wall, such as lipopolysaccharides and cell surface proteins, causing the disruption of cell wall and membranes, leakage of proteinaceous compounds, and other intracellular constituents from the microorganisms. [164][165][166] Another mechanism is associated with the interaction between chitosan and cellular DNA, inhibiting the transcription of DNA, RNA, and protein synthesis, culminating with cell death.…”

Section: Characteristicsmentioning

confidence: 99%

New Perspectives of Using Chitosan, Silver, and Chitosan–Silver Nanoparticles against Multidrug‐Resistant Bacteria

Polinarski

Beal

Silva

et al. 2021

Part & Part Syst Charact

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materials are typically developed at a critical length of under 100 nm. However, other phenomena, such as transparency and stable dispersion, can occasionally extend the upper limit, and the use of the prefix "nano" is accepted for dimensions smaller than 500 nm. [2] Two relevant factors differentiate nanomaterials from macroscopic materials: first, the quantum effect that can promote changes in physical properties, such as color and electrical conductivity, and second, the surface effect related to the increased surface area to volume ratio that favors physical and chemical interactions between atoms and the surrounding environment. [3] NPs are of the same order of magnitude as antibodies, membrane receptors, nucleic acids, proteins, and other biomolecules, thus representing a potential material to be used in medicine for imaging applications, diagnosis, therapies, or in medical devices. [4] The small size and large surface area of NPs facilitate their permeation through cell membranes and enhance their biological activity. [5] Microbial colonization of medical devices leads to the formation of microbial or fungal biofilms, which are complex assemblages of microbial cells associated with a surface and incorporated into an extracellular matrix. [6] Biofilms are critical clinical issues because they culminate in the pathogenesis of numerous bacterial infections that are difficult to treat and effectively eradicate with antibiotics. [7] Biofilm formation can occur in three stages: attachment, maturation, and dispersion. The attachment step can be further categorized in two-stage processes: initial reversible attachment and irreversible attachment, in which the attached biofilm can tolerate strong shear forces. [8] The bacteria deposition is mediated by sedimentation, Brownian motion, and hydrodynamic forces, whereas adhesion is governed by Van der Waals, acid-base, hydrophobic, and electrostatic interaction forces. [9] Attachment is the most crucial phase to prevent bacterial adhesion because the antimicrobial action becomes less effective after biofilm formation. [10] Medical devices commonly affected by microbial contamination and biofilm formation are catheters, probes, and wound dressings. Catheters are used in patients to administer fluids, blood products, and parenteral nutrition. [6] Most nosocomial infections in intensive care units (ICUs) are associated with the insertion and maintenance of central venous catheters Nanomaterials with antimicrobial activity are promising alternatives to overcome microbial resistance in medical devices. Catheters, probes, and wound dressings are among the medical devices mostly affected by microbial contamination and the formation of polymicrobial biofilms. Nanoparticles (NPs) derived from natural sources, such as chitosan nanoparticles (CsNPs), and metal-based nanoparticles, including silver nanoparticles (AgNPs), are receiving increased interest in nanomedicine. CsNPs have been widely explored as a coating material and antimicrobial agent. AgNPs have a strong antimicrobi...

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Antibacterial activities of hexadecanoic acid methyl ester and green‐synthesized silver nanoparticles against multidrug‐resistant bacteria

2021

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Antibacterial drug resistance is considered one of the biggest threats to human health worldwide, and the overuse of antibiotics accelerates this problem. Multidrug‐resistant (MDR) bacteria are becoming harder to treat as the antibiotics used to treat them become less effective. Therefore, it is necessary to evaluate novel methods to control MDR bacteria. In this study, 40 bacterial isolates were collected from diabetic patients. The sensitivity of 40 bacterial isolates to seven antibiotics was evaluated. Four bacterial isolates were resistant to all antibiotic groups. The MDR pathogenic bacteria were selected and identified morphologically and biochemically and confirmed by VITEK® 2 system as follows: Staphylococcus aureus W35, Pseudomonas aeruginosa D31, Klebsiella pneumoniae DF30, and K. pneumoniae B40. Identification of the most resistant P. aeruginosa D31 was confirmed by the sequencing of a 16S ribosomal RNA gene with an accession number (MW241596). The inhibitory activity of eight types of native grown plant extracts against MDR bacteria was studied. Clove alcoholic extract (CAE) showed the highest inhibitory activity against MDR bacteria. Gas chromatography–mass spectrometry analysis of partially purified CAE at 0.9 Rf detected by thin‐layer chromatography showed an active compound named hexadecenoic acid methyl ester with the highest antimicrobial effect against clinical pathogenic bacteria. The formation of silver nanoparticles (AgNPs) by CAE was studied. Evaluation of AgNPs was investigated by X‐ray diffraction, UV–Vis, and transmission electron microscopy. The antibacterial effect of AgNPs after 2, 4, and 6 days in light and dark conditions was evaluated. Finally, the AgNPs synthesized using CAE possess good inhibition activity against the tested pathogenic bacteria. As a result, the bactericidal components listed above were promising in reducing MDR bacteria and can be used for treatments of bacterial infection and in the development of safe products with a natural base.

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Antibacterial, anticoagulant and cytotoxic evaluation of biocompatible nanocomposite of chitosan loaded green synthesized bioinspired silver nanoparticles

Cited by 66 publications

References 49 publications

<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

<p>Synergistic Nanocomposites of Different Antibiotics Coupled with Green Synthesized Chitosan-Based Silver Nanoparticles: Characterization, Antibacterial, in vivo Toxicological and Biodistribution Studies</p>

New Perspectives of Using Chitosan, Silver, and Chitosan–Silver Nanoparticles against Multidrug‐Resistant Bacteria

Antibacterial activities of hexadecanoic acid methyl ester and green‐synthesized silver nanoparticles against multidrug‐resistant bacteria

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