Antibacterial drug resistance is considered one of the biggest threats to human health worldwide, and the overuse of antibiotics accelerates this problem. Multidrug‐resistant (MDR) bacteria are becoming harder to treat as the antibiotics used to treat them become less effective. Therefore, it is necessary to evaluate novel methods to control MDR bacteria. In this study, 40 bacterial isolates were collected from diabetic patients. The sensitivity of 40 bacterial isolates to seven antibiotics was evaluated. Four bacterial isolates were resistant to all antibiotic groups. The MDR pathogenic bacteria were selected and identified morphologically and biochemically and confirmed by VITEK® 2 system as follows: Staphylococcus aureus W35, Pseudomonas aeruginosa D31, Klebsiella pneumoniae DF30, and K. pneumoniae B40. Identification of the most resistant P. aeruginosa D31 was confirmed by the sequencing of a 16S ribosomal RNA gene with an accession number (MW241596). The inhibitory activity of eight types of native grown plant extracts against MDR bacteria was studied. Clove alcoholic extract (CAE) showed the highest inhibitory activity against MDR bacteria. Gas chromatography–mass spectrometry analysis of partially purified CAE at 0.9 Rf detected by thin‐layer chromatography showed an active compound named hexadecenoic acid methyl ester with the highest antimicrobial effect against clinical pathogenic bacteria. The formation of silver nanoparticles (AgNPs) by CAE was studied. Evaluation of AgNPs was investigated by X‐ray diffraction, UV–Vis, and transmission electron microscopy. The antibacterial effect of AgNPs after 2, 4, and 6 days in light and dark conditions was evaluated. Finally, the AgNPs synthesized using CAE possess good inhibition activity against the tested pathogenic bacteria. As a result, the bactericidal components listed above were promising in reducing MDR bacteria and can be used for treatments of bacterial infection and in the development of safe products with a natural base.
The emergence of types of bacteria resistant to antibiotics is a common and most dangerous problem in the current era. In this study 50 samples from diabetic patients including diabetic foot (n = 36), wounds (n = 9), and bedsores (n = 5) were isolated and grew well on (nutrient agar, blood agar, and MacConkey gar) media. Ten patient's specimens (20%) had no bacterial growth. The sensitivity of 40 bacterial isolates to 7 antibiotics was performed. A microscopic examination of 40 bacterial isolates by Gram stained film was performed. Morphological and biochemical identifications of selected MDR bacteria were evaluated. The four MDR pathogenic bacteria are named S. aureus W, P. aeruginosa DF, K. pneumonia D, and K. pneumonia BS. Determination of MIC and MBC of the 3 strongest antibiotics against 4 multidrug resistant pathogenic bacteria. Antibacterial activities of six types of natural plant extracts were studied against tested pathogenic bacteria. Clove, garlic, and cinnamon boiling water extract showed inhibitory activity against MDR bacteria.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.