Antibacterial, anti-inflammatory, and anti-osteoclastogenic activities of &lt;i&gt;Colocasia antiquorum&lt;/i&gt; var. &lt;i&gt;esculenta&lt;/i&gt;: Potential applications in preventing and treating periodontal diseases

Moon, Sang‐Jin; Ji, Sang Hee; Son, Ju-Lee; Shin, Seong-Jin; Oh, Seunghan; Kim, Seong Hwan; Bae, Ji-Myung

doi:10.4012/dmj.2020-157

Cited by 5 publications

(6 citation statements)

References 40 publications

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“…We demonstrated that 75% ethanolic extract of CA has antibacterial activity against P. gingivalis 33277. In a previous study, the antibacterial activity of CA methanolic extract inhibited the growth of P. gingivalis by 40% [ 17 ]. We found that the MIC of 75% ethanolic extract of CA was lower than that of CA methanolic extract.…”

Section: Discussionmentioning

confidence: 99%

“…CA (Namwon-si, Korea) extract was purchased from the Korea Plant Extract Bank of the Korea Research Institute of Bioscience and Biotechnology (KRIBB, Daejeon, Korea). The method for preparing the extract was described in a previous study [ 17 ] except the extraction solvent was 75% ethyl alcohol. The plant was dried in the shade and powdered, and the powder was added to 1 L of ethyl alcohol 75% (GR Grade) and extracted through 30 cycles (40 KHz, 1500 W, 15 min ultrasonication, and 120 min standing per cycle) at room temperature using an ultrasonic extractor (SDN-900H, SD-ULTRASONIC CO., LTD., Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Two hours before the first inoculation, VA and VCA groups were anesthetized with urethane (Daejung, Siheung, Korea), and varnish was applied for 15 s to both maxillary molars. The varnish used in this study was described in a previous study [ 17 ]. In the VCA group, varnish was mixed with 15% CA.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, the methanol extract of Colocasia antiquorum var. esculenta (CA) was confirmed to have antibacterial and anti-inflammatory effects and to inhibit osteoclast differentiation [ 17 ]. However, no studies have evaluated the efficacy of CA in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Colocasia antiquorum var. Esculenta Extract In Vitro and In Vivo against Periodontal Disease

et al. 2021

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Background and Objectives: Periodontal disease is a chronic inflammatory disease in which gradual destruction of tissues around teeth is caused by plaque formed by pathogenic bacteria. The purpose of this study was to evaluate the potential of 75% ethanol extract of Colocasia antiquorum var. esculenta (CA) as a prophylactic and improvement agent for periodontal disease in vitro and in vivo. Materials and Methods: The antimicrobial efficacy of CA against Porphyromonas gingivalis (P. gingivalis, ATCC 33277) was evaluated using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) test, and cytotoxicity was confirmed by CCK-8 assay. For the in vivo study, P. gingivalis was applied by oral gavage to BALB/c mice. Forty-two days after the first inoculation of P. gingivalis, intraoral swabs were taken for microbiome analysis, and the mice were sacrificed to evaluate the alveolar bone loss. Results: The MIC of CA against P. gingivalis was 31.3 μg/mL, the MBC was 62.5 μg/mL, with no cytotoxicity. The diversity of the oral microbiome decreased in the positive control group, while those of the VA (varnish) and VCA (varnish added with CA) groups increased as much as in the negative control group, although the alveolar bone loss was not induced in the mouse model. Conclusions: CA showed antibacterial effects in vitro, and the VA and VCA groups exhibited increased diversity in the oral microbiome, suggesting that CA has potential for improving periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Colocasia antiquorum var. Esculenta Extract In Vitro and In Vivo against Periodontal Disease

et al. 2021

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“…For determining the sustainability of antibacterial activity, an experimental fluoride varnish (FV) was fabricated as a carrier for A. incisum . The experimental FV was formulated according to a previously reported method [ 17 ]. Briefly, the experimental FV was composed of 45 wt.% rosin, 50 wt.% ethanol, and 5 wt.% sodium fluoride.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effect of Asplenium incisum on Bacterial Growth, Inflammation, and Osteoclastogenesis

et al. 2021

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Background and Objectives:Asplenium incisum, a natural plant, is known to possess numerous pharmacological and biochemical properties. However, the inhibitory effect of A. incisum against Porphyromonas gingivalis and other factors related to periodontal disease have not yet been demonstrated. This study aimed to investigate the potential of A. incisum extract as a phytotherapeutic candidate for improving periodontal diseases by assessing its antibacterial, anti-inflammatory, and anti-osteoclastogenic activities. Materials and Methods: The inhibition of proliferation of P. gingivalis by A. incisum and the sustainability of its antibacterial activity were evaluated in this study. The production of inflammatory cytokines (tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)) and nitric oxide (NO) from lipopolysaccharide-stimulated RAW 264.7 cells was assessed using an enzyme-linked immunosorbent assay. To identify the anti-osteoclastogenic activity, tartrate-resistant acid phosphatase (TRAP) staining and TRAP activity analyses were performed on bone marrow macrophages. Results: The proliferation of P. gingivalis was significantly inhibited by A. incisum (p < 0.001), and the antibacterial activity was sustained for up to 3 days. A. incisum showed anti-inflammatory activities by significantly decreasing the release of TNF-α, IL-6 (p < 0.05), and NO (p < 0.01). In addition, A. incisum significantly suppressed TRAP-positive cells and TRAP activity (at 30 μg/mL, p < 0.01) without causing any cytotoxicity (p > 0.05). Conclusions:A. incisum showed antibacterial, anti-inflammatory, and anti-osteoclastogenic activities, suggesting it has strong therapeutic potential against periodontal diseases.

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Exploring the osteogenic potential of chitosan-quercetin bio-conjugate: In vitro and in vivo investigations in osteoporosis models

Li,

Selvaraj,

Saravanan

et al. 2024

International Journal of Biological Macromolecules

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Antibacterial, anti-inflammatory, and anti-osteoclastogenic activities of <i>Colocasia antiquorum</i> var. <i>esculenta</i>: Potential applications in preventing and treating periodontal diseases

Cited by 5 publications

References 40 publications

Effects of Colocasia antiquorum var. Esculenta Extract In Vitro and In Vivo against Periodontal Disease

Effects of Colocasia antiquorum var. Esculenta Extract In Vitro and In Vivo against Periodontal Disease

Inhibitory Effect of Asplenium incisum on Bacterial Growth, Inflammation, and Osteoclastogenesis

Exploring the osteogenic potential of chitosan-quercetin bio-conjugate: In vitro and in vivo investigations in osteoporosis models

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