Antibacterial and Mechanical Assays of Resin Modified Glass Ionomer Containing Propolis Extract

Abstract

The antibacterial efficacy of restorative materials had an important role in preventing the recurrent caries. The objective of this study was to evaluate in-vitro the antibacterial and mechanical assays of Vitremer containing ethanolic extract of propolis (EEP). Materials and methods: I-Antibacterial Assay: The standard strain of Streptococcus mutans and Lactobacillus acidophilus were used for determination of minimum inhibitory concentration (MIC) of EEP/Vitremer. II-Mechanical Assay: A-Shear bond strength (SBS): sixty half-crowns of non-carious extracted 2 nd primary molars were placed at standard moulds containing Teflon disc that had 4mm x 3mm central hole and divided into 4 groups (n=15) according to the different EEP concentrations. Group I: 10%, group II: 25%, group III: 50% EEP-Vitremer mixture, and group IV (control): 0% EEP/ Vitremer and SBS was assessed using Instron machine. B-Microhardness: Sixty standard discshaped specimens were prepared from mixture 0%, 10%, 25%, and 50%, n=15 and nanoindentation value was recorded. Data were analyzed using one-way ANOVA and post-hoc test. Results: Only MIC of 10%, 25%, and 50% mixture showed growth inhibition against S. mutans, compared to 25% and 50% against L. acidophilus. SBS showed that 0% EEP recorded the highest value followed by10% mixture but the difference was not significant (p>0.05), while 25% and 50% reported the lowest values and the differences were significant (p< 0.05). 25% and 50% mixtures recorded the highest significant microhardness (p<0.05). 0% EEP and 10% mixtures displayed no significant differences between them (P>0.05). Conclusions: 25% EEP-Vitremer mixture was the most suitable concentration as it exhibits positive significant antibacterial and mechanical assays.

Antibacterial Effect of Pre-constructed 3D Bone Scaffolds before and after Modification with Propolis

“…In the current study, both scaffold materials were modified with EEP 15% and 25% by weight, where 25% EEP mixture is the optimum concentration as it exhibits positive significant antibacterial activity without adversely affecting the mechanical properties, in addition, increasing the EEP incorporation more than 25% weakens the scaffold and negatively affects the physical properties of the mixture; it prolongs the working time and interferes with the network formation [17].…”

Evaluasi Perubahan Warna Semen Ionomer Kaca dengan Penambahan Ekstrak Etanol Propolis Trigona spp.

“…4,5 Secara in vitro, ion fluor ditemukan memiliki efek antikariogenik melalui penurunan demineralisasi dan peningkatan remineralisasi melalui konversi hidroksiapatit menjadi fluorapatit yang lebih tahan terhadap asam, dan dengan menghambat pembentukan dan metabolisme bakteri. 6,7 Akan tetapi, relevansi klinis restorasi yang melepaskan fluor masih diperdebatkan. Hasil dari beberapa studi klinis mengenai kemampuan fluor yang dilepaskan dalam mengurangi kejadian karies sekunder memberikan hasil yang tidak konsisten.…”

“…8 Oleh karena itu, SIK sering dimodifikasi dengan agen antibakteri untuk meningkatkan efek terapeutiknya. 7 Propolis merupakan bahan resin lengket yang dihasilkan lebah Trigona spp. dengan mencampurkan sekresi saliva, beeswax, dan resin dari berbagai tanaman.…”

Evolution of Anticariogenic Resin‐Modified Glass Ionomer Cements

“…Talat and Marwa used a similar ethanolic extract of propolis (EEP) and added it to RMGIC 75. RMGIC was chosen because of its biocompatible nature and better mechanical strength than GIC 76.…”