Antibacterial and antioxidant properties of phyto‐synthesized silver nanoparticles using Lavandula stoechas extract

Abstract

Due to environmentally friendly and cost‐ effective issues, biological methods for silver nanoparticles (AgNPs) synthesis are advantageous over chemical and physical ones. In this study, AgNPs synthesized using Lavandula stoechas extract as a reductant and its antioxidant capacity, antibacterial property and cytotoxicity effect were investigated. The phyto‐synthesized AgNPs were characterized using various analyses such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), x‐ray diffraction (XRD) as well as Fourier transform infrared (FT‐IR). The prepared nanoparticles were spherical on shape with the size about 20–50 nm. Antibacterial studies through agar disk diffusion method confirmed the antibacterial potential of phyto‐synthesized AgNPs toward two clinical Staphylococus aureus and Pseudomonas aeruginosa bacteria, although MTT assay demonstrated that S. aureus (MIC = 125 μg/ml) was more susceptible to AgNPs than P. aeruginosa (MIC = 250 μg/ml). Moreover, the cytotoxicity assay of phyto‐synthezied AgNPs showed a low cytotoxic effect on RAW264 cell line at 62.5 μg/ml as an effective concentration. Also the considerable antioxidant capacity of the AgNPs confirmed through DPPH assay. Great antibacterial and antioxidant properties along with biocompatibility make the suggested phyto‐synthesized AgNPs a great candidate for different biomedical applications including wound healing.

Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system)

“…AgNPs synthesised using Aloin extracted from Aloe barbadensis was evaluated for its cytotoxic potential against MCF-7 cell line which showed an IC 50 value of 22 lg/ml [49] while Das et al [50] reported cytotoxic effect of Ananas comosus mediated synthesised AgNPs against HepG 2 cell lines. Mahmoudi et al [51] reported cytotoxic effect of AgNPs synthesised using Lavandula stoechas extract on RAW264 cell line at 62.5 lg/ml as an effective concentration. In fact, green synthesised AgNPs show promising cytotoxic effect against an array of cell lines like Jurkat, MCF, AGS, Hep3B, MG-63, HT29, U87, A549, HeLa, HeK293, SNO, MKN-28, etc.…”

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

“…Therefore, alternatives less hazardous and economically viable methods are being explored. Many reports are available on the biogenesis of AgNPs using several plant extracts 25 .…”

Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

“…The MTT assay is widely used for the analysis of cytotoxicity caused by silver nanoparticles [70,71], and has demonstrated that the cytotoxic effect depends directly on the physical-chemical characteristics of the nanoparticles, their concentration, the exposure time, and the target cell.…”