Antibacterial and Antifungal Activity of Functionalized Cotton Fabric with Nanocomposite Based on Silver Nanoparticles and Carboxymethyl Chitosan

Abstract

Cotton is the most widely used natural fiber for textiles; however, the capacity of cotton fibers to absorb large amounts of moisture, retain oxygen, and have a high specific surface area makes them more prone to microbial contamination, becoming an appropriate medium for the growth of bacteria and fungi. In recent years, the incorporation of silver nanoparticles in textile products has been widely used due to their broad-spectrum antibacterial activity and low toxicity towards mammalian cells. The aim of the current study is to continue the assessment of our developed nanocomposite and evaluate the antibacterial and antifungal activity of the nanocomposite based on silver nanoparticles and carboxymethyl chitosan (AgNPs-CMC) against Escherichia coli, Staphylococcus aureus, and Candida albicans, evaluated by the well diffusion method. The antibacterial activity against E. coli and S. aureus was also evaluated by the qualitative method of inhibition zone and the quantitative method of colony counting. Likewise, the antifungal activity of the functionalized fabric against Candida albicans and Aspergillus niger was determined by the inhibition zone method and the antifungal activity method GBT 24346-2009, respectively. The functionalized fabric showed 100% antibacterial activity against E. coli and S. aureus and good antifungal activity against C. albicans and A. niger. Our results indicate that the functionalized fabric could be used in garments for hospital use to reduce nosocomial infections.

Green Synthesis of a Novel Silver Nanoparticle Conjugated with Thelypteris glandulosolanosa (Raqui-Raqui): Preliminary Characterization and Anticancer Activity

“…The functionalized fabric evaluated using X-ray diffraction (XRD) analysis further confirmed the presence of silver nanoparticles [132]. Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) determined an average concentration of 13.5 mg of silver per kg of functionalized fabric [132]. IR reported that the functionalized fabric variation had a displaced peak of intensity at 1594.32 cm −1 , corresponding to carboxylate anions [132].…”

“…Scanning electron microscopy (SEM) demonstrated that the silver nanoparticles were spherical in shape and between 5 and 20 nm [131]. The functionalized fabric evaluated using X-ray diffraction (XRD) analysis further confirmed the presence of silver nanoparticles [132]. Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) determined an average concentration of 13.5 mg of silver per kg of functionalized fabric [132].…”

“…Our research group has previously synthesized and characterized functionalized cotton fabric with silver nanoparticles and carboxymethyl chitosan (AgNPs-CMC) [131,132]. The nanocomposite obtained from the complex [Ag (NH 3 ) 2 ] + was synthesized under similar conditions and verified the formation of silver nanoparticles [131].…”

“…Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) determined an average concentration of 13.5 mg of silver per kg of functionalized fabric [132]. IR reported that the functionalized fabric variation had a displaced peak of intensity at 1594.32 cm −1 , corresponding to carboxylate anions [132]. Similarly, Raman spectroscopy demonstrated an intense peak at 1592.84 cm −1 , which is characteristic and corresponds to the primary amino group of carboxymethyl chitosan, and a peak at 1371.5 cm −1 corresponding to the carboxylic anions [132].…”

“…IR reported that the functionalized fabric variation had a displaced peak of intensity at 1594.32 cm −1 , corresponding to carboxylate anions [132]. Similarly, Raman spectroscopy demonstrated an intense peak at 1592.84 cm −1 , which is characteristic and corresponds to the primary amino group of carboxymethyl chitosan, and a peak at 1371.5 cm −1 corresponding to the carboxylic anions [132]. Finally, the physical and mechanical tests of tensile strength and color index were similar [132].…”