Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Júnior, Sérgio Dias da Costa; Santos, João Victor de Oliveira; Campos, Luís André de Almeida; Pereira, Marcela Araújo; Magalhães, Nereide Stela Santos; Cavalcanti, Isabella Macário Ferro

doi:10.22161/ijeab/3.5.50

Cited by 26 publications

(18 citation statements)

References 24 publications

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“…However, neither 64 μg/ml nor 128 μg/ml impaired the growth of S. aureus, which is consistent with the previous studies (Carrada and Sánchez, 2017;Wang et al, 2019). This differs from the results of Júnior et al, showing that 250-500 μg/ml QEN inhibited 50% of biofilm formation in the MRSA bacterial strains and is similar to the 50 μg/ml concentration of MSSA ATCC 6538 (Lee et al, 2013;Júnior et al, 2018). To further determine whether QEN affects the growth of the S. aureus, the cell morphology of S. aureus was observed using transmission electron microscopy, and QEN was found to trigger the sparseness of the S. aureus cell wall structure, but the division and proliferation were not affected.…”

Section: Discussionsupporting

confidence: 89%

“…Prior to determining whether QEN affected S. aureus biofilm formation, available literature revealed that the MBIC of QEN on the biofilm ranged from 9 μg/ml to 60 μg/ml (Amin et al, 2015;Júnior et al, 2018;Mohamed et al, 2020). Therefore, we established 0 μg/ml, 8 μg/ml, 16 μg/ml, 32 μg/ml, 64 μg/ml, 128 μg/ml concentrations gradient to examine their MBIC value .…”

Section: The Effect Of Quercetin On Biofilm Formation In Staphylococcus Aureusmentioning

confidence: 99%

“…Quercetin (QEN) is a plant metabolite, a polyphenolic flavonoid found widely in fruits, vegetables, nuts, seeds, bark, flowers, and leaves (Williamson and Manach, 2005;Wiczkowski et al, 2008). The available data indicate QEN significantly inhibited the S. aureus biofilm formation (Lee et al, 2013), but studies obtained various MICs (Cho et al, 2015;Júnior et al, 2018). Compared to the other flavonoids, QEN with five phenolic hydroxyl groups has the most substantial inhibitory effect (Cho et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Potential Mechanisms of Quercetin Influence the ClfB Protein During Biofilm Formation of Staphylococcus aureus

Kang

Wang

et al. 2022

Front. Pharmacol.

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This study aimed to establish the mode of binding between Quercetin (QEN) and an essential protein called ClfB in forming biofilm in Staphylococcus aureus (S. aureus). In this study, the raw data of GSE163153 were analyzed for quality control, alignment, and gene counts, and the differential analysis detected the key differentially expressed genes (DEGs) assisting in the formation of the S. aureus biofilm. Then, the protein-protein interaction (PPI) and gene function enrichment analyses of the target genes, identified a gene called clfB to be closely related to biofilm formation. ClfB was structurally characterized, molecularly docked, and kinetically simulated to unravel the mode of binding of QEN to ClfB. Meanwhile, the growth curve and transmission electron microscopy methods examined the effect of QEN on the S. aureus growth. Results indicated that the clfB gene was increasingly expressed during biofilm formation and was involved in cell adhesion, pathogenicity, and infection. We identified 5 amino acid sites of ClfB (D272, R331, I379, K391, E490) as potential sites for binding QEN, which would indirectly influence the changes in the functional sites N234, D270, Y273, F328, inhibiting the formation of biofilm. Meanwhile, 128 μg/ml of QEN could significantly inhibit the S. aureus biofilm formation. This manuscript serves as a molecular foundation for QEN as an antibacterial drug providing a new perspective for developing antibacterial drugs.

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Section: Discussionsupporting

confidence: 89%

Section: The Effect Of Quercetin On Biofilm Formation In Staphylococcus Aureusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential Mechanisms of Quercetin Influence the ClfB Protein During Biofilm Formation of Staphylococcus aureus

Kang

Wang

et al. 2022

Front. Pharmacol.

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“…The best inhibitory effect of QRC was observed against the methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-intermediate Staphylococcus aureus (VISA) strains (MIC = 250, 500 and 125 µg/mL, respectively), while the lowest inhibitory effect was against the vancomycin-resistant Staphylococcus aureus (VRSA) strain (MIC = 1000 µg/mL). Regarding the antibiofilm activity, QRC was able to reduce 50% of the biofilm production, even when it was analysed in sub inhibitory concentrations [40].…”

Section: The Antibacterial Effect Of Quercetinmentioning

confidence: 96%

Quercetin-Based Nanocomposites as a Tool to Improve Dental Disease Management

et al. 2020

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The restoration and prosthetic rehabilitation of missing teeth are commonly performed using dental implants, which are extremely effective and long-lasting techniques due to their osteointegration ability with the preimplant tissues. Quercetin is a phytoestrogen-like flavonoid well known for its several positive effects on human health, mostly linked to the anti-inflammatory, antioxidant, and antibacterial activities against both Gram-positive and Gram-negative bacteria. Moreover, many studies in dentistry and the maxillofacial fields have highlighted the positive effects of quercetin on osteogenesis, acting on osteoblast activity and angiogenetic process, and promoting soft and hard tissue regeneration. This review focuses on the role of quercetin on the healing and restoration of bony defects, considering the experimental findings of its application both in vitro and in vivo as a mere compound or in association with scaffolds and dental implants having functionalized surfaces.

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“…Quercetin is a major flavonoid content in most plant extracts ( Junior et al, 2018 ). Quercetin is found in apples, red grapes, kales, broccoli, onions, berries, and cherries ( Xu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Quercetin prevents chronic kidney disease on mesangial cells model by regulating inflammation, oxidative stress, and TGF-β1/SMADs pathway

Widowati

Prahastuti

Tjokropranoto

et al. 2022

PeerJ

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Background Chronic kidney disease (CKD) happens due to decreasing kidney function. Inflammation and oxidative stress have been shown to result in the progression of CKD. Quercetin is widely known to have various bioactivities including antioxidant, anticancer, and anti-inflammatory activities. Objective To evaluate the activity of quercetin to inhibit inflammation, stress oxidative, and fibrosis on CKD cells model (mouse mesangial cells induced by glucose). Methods and Material The SV40 MES 13 cells were plated in a 6-well plate with cell density at 5,000 cells/well. The medium had been substituted for 3 days with a glucose-induced medium with a concentration of 20 mM. Quercetin was added with 50, 10, and 5 µg/mL concentrations. The negative control was the untreated cell. The levels of TGF-β1, TNF-α, and MDA were determined using ELISA KIT. The gene expressions of the SMAD7, SMAD3, SMAD2, and SMAD4 were analyzed using qRT-PCR. Results Glucose can lead to an increase in inflammatory cytokines TNF-α, TGF-β1, MDA as well as the expressions of the SMAD2, SMAD3, SMAD4, and a decrease in SMAD7. Quercetin caused the reduction of TNF-α, TGF-β1, MDA as well as the expression of the SMAD2, SMAD3, SMAD4, and increased SMAD7. Conclusion Quercetin has anti-inflammation, antioxidant, antifibrosis activity in the CKD cells model. Thus, quercetin is a promising substance for CKD therapy and further research is needed to prove this in CKD animal model.

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Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Cited by 26 publications

References 24 publications

Potential Mechanisms of Quercetin Influence the ClfB Protein During Biofilm Formation of Staphylococcus aureus

Potential Mechanisms of Quercetin Influence the ClfB Protein During Biofilm Formation of Staphylococcus aureus

Quercetin-Based Nanocomposites as a Tool to Improve Dental Disease Management

Quercetin prevents chronic kidney disease on mesangial cells model by regulating inflammation, oxidative stress, and TGF-β1/SMADs pathway

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