Antibacterial and antibiofilm activities of the metabolites isolated from the culture of the mangrove-derived endophytic fungus Eurotium chevalieri KUFA 0006

Chromone Derivatives and Other Constituents from Cultures of the Marine Sponge-Associated Fungus Penicillium erubescens KUFA0220 and Their Antibacterial Activity

“…The structure of β-sitostenone [3], ergosterol 5,8-endoperoxide [4] (Figure S1), citromycin ( 1a ) [5], 12-methoxycitromycin ( 1b ) [5], myxotrichin D ( 1d ) [6], 12-methoxycitromycetin ( 1e ) [5], anhydrofulvic acid ( 2a ) [7,8], myxotrichin C ( 2b ) [6], penialidin D ( 2c ) [9], penialidin F ( 3a ) [9,10], SPF-3059-30 ( 7 ) [11], GKK1032B ( 8 ) [12,13,14], and secalonic acid A ( 9 ) [3,15] (Figure 1) were elucidated by analysis of their 1D and 2D NMR spectra as well as HRMS data, and also by comparison of their spectral data (Figures S2–S11, S15–S29, S45–S50, S52 and S53) to those reported in the literature. In the case of GKK1032B ( 8 ), the X-ray analysis was also performed to confirm the absolute configurations of all the stereogenic centers (Figure S51).…”

“…Frs 238–245 were combined (1.75 g) and precipitated in MeOH to give 202.1 mg of 8 [12,13,14]. Frs 246–251 were combined (2.67 g) and precipitated in MeOH to give 472.2 mg of ergosterol 5,8-endoperoxide [4]. Frs 252–286 were combined (493.0 mg) and crystallized in MeOH to give further 367.1 mg of ergosterol 5,8-endoperoxide.…”

“…Chromatographic fractionation and the further purification of the crude ethyl acetate extract of the cultures of P. erubescens KUFA 0220, furnished an unreported chromene derivative, 1-hydroxy-12-methoxycitromycin ( 1c ), and four previously undescribed chromone derivatives, including a pyanochromone ( 3b ), a spirofuranochromone ( 4 ), 7-hydroxy-6-methoxy-4-oxo-3-[(1 E )-3-oxobut-1-en-1-yl]-4 H -chromene-5-carboxylic acid ( 5 ), and a pyranochromone dimer ( 6 ) (Figure 1), in addition to thirteen known compounds: β-sitostenone [3,4], citromycin ( 1a ) [5], 12-methoxycitromycin ( 1b ) [5], myxotrichin D ( 1d ) [6], 12-methoxycitromycetin ( 1e ) [5], anhydrofulvic acid ( 2a ) [7,8], myxotrichin C ( 2b ) [6], penialidin D ( 2c ) [9], penialidin F ( 3a ) [9,10], SPF-3059-30 ( 7 ) [11], GKK1032B ( 8 ) [12,13,14] and secalonic acid A ( 9 ) [3] (Figure 1). The structures of the previously undescribed compounds were established based on extensive analyses of their 1D and 2D NMR as well as HRMS data while the identity of the known compounds was elucidated by comparison of their 1 H and 13 C NMR data with those reported in the literature.…”

Long-Term Effect against Methicillin-Resistant Staphylococcus aureus of Emodin Released from Coaxial Electrospinning Nanofiber Membranes with a Biphasic Profile

“…Emodin,1,3,8-trihydroxy-6-methyl-anthraquinone ( Figure 1A), a common chemical component found in many natural plants [8], has outstanding antimicrobial [9,10], anticancer [11], antioxidant [12], and anti-inflammatory [13,14] activities. It has attracted enormous attention due to its potent inhibition effect on methicillin-sensitive S. aureus and MRSA related to pyruvate pathway imbalance induction, protein synthesis inhibition, and DNA synthesis suppression [10].…”

Reduction ofBacillus cereusBiofilms on Stainless Steel Surfaces byPestalotiopsissp. Culture Crude Extract

“…Examples of these include the new phenyl derivatives isolated from an endophytic fungus, Aspergillus flavipes, which were shown to penetrate through the biofilm matrix and kill live cells inside mature Staphylococcus aureus biofilms (Bai et al, 2014). Other examples include the bioactive specialized metabolites extracted from a mangrove-derived endophytic fungus, Eurotium chevalieri, which were shown to inhibit biofilms of S. aureus and Escherichia coli (Zin et al, 2017). Presently, there have been no specific studies conducted on the antibiofilm activities of secondary metabolites extracted from Pestalotiopsis sp.…”