Antibacterial and anti-ulcerogenic effects of Punicagranatum peel extract against ethanol-induced acute gastric lesion in rats

Abstract

The potential therapeutic properties of indigenous plants have been studied worldwide and were used either for treatment or prevention of various diseases. Punicagranatum (pomegranate), a plant from family Lythraceae, has been reported to enclose many medical properties like chemopreventive, 1 antioxidant, 2 antifungal, 3 anti-inflammatory, 4 antibacterial, 5 and wound healing. 6 It also has been reported to have a preventive role against obesity. 7 Phytochemical screening of Punicagranatum extract found to contain steroids, triterpenoids, saponins, glycosides, flavonoids, alkaloids, carbohydrate tannins, and vitamin C. 8 The red color of juice can be pointed to anthocyanins such as cyanidin, delphinidin and pelargonidin glycosides. 9,10 The peel contains three times more polyphenols compared to the pulp such as catechins, condensed t a n n i n s 1 1 g a l l o c a t e c h i n s a n d prodelphinidins. 12 This study aimed to study the antibacterial and anti-ulcerogenic activities of Punicagranatum peel extract on ethanol induced gastric lesions on experimental rats.Background and objectives: Punicagranatum (pomegranate) is a fruit-bearing deciduous shrub or small tree from the family Lythraceae. It has been cultivated since ancient times throughout the Mediterranean region. Different parts of it have been used for research, such as fruits, peels, and juice. This study aimed to investigate the antibacterial and anti-ulcer effect of Punicagranatum peel extract and to screen the expression of transforming growth factor β1 (TGF β1) in rat's blood serum after stomach ulcer was induced by ethanol. Methods: Twenty four rats were divided randomly into 4 groups; GroupI(ulcer positive group) rats were (experimental) orally administered 5 ml/kg sterilized distilled water (the vehicle). Group II (ulcer negative group) rats were orally administered 5 ml/kg of 20 mg/kg esomeprazole. Groups III and groups IV were orally administered 5 ml/kg of 250 and 500 mg/kg of Punicagranatum plant extract, respectively. Results: Data showed an antibacterial activity of Punicagranatum peel extract against gram positive and gram negative bacteria and the best antibiotic in which both bacteria were sensitive to was norfloxacin(10 µg). Treatment with Punicagranatum peel extract and esomeprazole had protective effects on stomach gastric mucosa, which indicate an anti-ulcer effect confirmed by the high levels of TGFβ1 in serum. Conclusion:We conclude that Punicagranatum peel extract exhibit antibacterial effect and possess a protective role against ethanol induced gastric ulcer in rats.

The Role of Fruit by-Products as Bioactive Compounds for Intestinal Health

“…This anti-ulcer impact was asserted by the high levels of TGFβ1 in serum and the decrease of ulcer lesion areas in the stomach layer. This significant antiulcer effect might be associated to existence of several bioactive compounds such as anthocyanins, anthocyanidins, punicic acid, ellagic acid, flavonoids and ellagitannins [ 152 ].…”

Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract

“…Thereafter, 30 µL of this extract were used to impregnate each of the sterile blank disks. These disks were left to dry, then placed on the H. pylori culture plates and incubated at 37°C for 1 h [9]. Disks with standard antibiotics (clarithromycin 15 µg, metronidazole 5 µg, ciprofloxacin 5 µg, and tetracycline 30 µg) were used as positive controls whereas, disks impregnated in a 2∶1 (v/v) DMSO-PBS (pH 6.8) only were used as negative controls [1].…”

“…Minimum inhibitory concentration of PPEE against clinical strains of Helicobacter pylori(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and the control strain.…”

Gastro-protective and therapeutic effect of Punica granatum against stomach ulcer caused by Helicobacter Pylori

“…A preventive role against obesity has also been identified. 7 Steroids, triterpenoids, saponins, glycosides, flavonoids, alkaloids, carbohydrate tannins and vitamin C have been found to contain the phytochemical screening of Punica granatum extract (10). Omeprazole is used for the treatment of conditions in which the stomach excretes large quantities of acid.…”