Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract

Abstract

Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect of its extract along with metronidazole against H. pylori. Materials and Methods: Pomegranate peel ethanol extracts (PPEE) was tested against a control strain of H. pylori (NCTC 11916) in vitro and in vivo in female Wistar rats. Moreover, the synergistic effect of PPEE in combination with metronidazole was tested in vitro. Results: The PPEE exhibited a remarkable activity against H. pylori with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. Furthermore, the extract exhibited a pronounced urease inhibitory activity (IC50 ∼6 mg/mL) against the tested strain. A synergistic effect between PPEE and metronidazole was also observed (fractional inhibitory concentrations <0.5). Oral treatment of rats with PPEE for 8 days produced a significant reduction in H. pylori gastritis and a significant decrease in both lymphocytic and positive chronicity. Conclusion: Pomegranate extract is probably safe and represents a potential alternative and complementary therapy for reducing H. pylori associated with gastric ulcers.

Synergistic In Vitro Antimicrobial Activity of Pomegranate Rind Extract and Zinc (II) against Micrococcus luteus under Planktonic and Biofilm Conditions

“…In this study, the PRE/Zn (II) combination exerted potentiated activity in reducing colonization and inhibiting growth control. The anti-adhesive activity of PRE has been confirmed in the literature [77,78]. PRE's activity on biofilm formation, inhibition and eradication could support the potential efficacy of PRE and the PRE/Zn (II) combination as a preventive agent in catheter-related infectious diseases caused by M. luteus, e.g., as a coating on the catheter surface prior to use.…”

Chemical profiling of Punica granatum peels from Jordan using LC–MS/MS and study on their biological activities

“…Also, it was mentioned that P. granatum peel extracts revealed higher biological properties than extracts obtained from several other parts of pomegranate (except that of flower) (Fischer et al., 2011). Interestingly, a recent study reported that ethanol pomegranate peel extract of Jordanian origin was well established to reduce Helicobacter pylori infection (Mayyas et al., 2021). However, there are no studies regarding the chemical composition and antioxidant activity of pomegranate peel in Jordan.…”

Evaluation of the synergistic antimicrobial effect of folk medicinal plants with clindamycin against methicillin‐resistant Staphylococcus aureus strains

“…The MIC of each plant extract and clindamycin were determined using a microdilution method (Mayyas et al 2021). All tests were done in triplicate using 96-well microplates.…”