Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing

Ninan, Neethu; Forget, Aurélien; Shastri, V. Prasad; Voelcker, Nicolas H.; Blencowe, Anton

doi:10.1021/acsami.6b10491

Cited by 492 publications

(336 citation statements)

References 68 publications

(110 reference statements)

Supporting

Mentioning

328

Contrasting

Unclassified

Order By: Relevance

“…Such structured 3D environments could allow the spatial guidance of cell differentiation by patterned mechanical cues within a hydrogel environment and enable the incorporation of mechanobiology paradigms in the direct 3DP of cells. Furthermore, our study extends the already broad utility of carboxylated agarose in biomedical applications which include synthetic 3D cell culture matrix, [2] nonwoven antimicrobial wound dressing, [39] and antimicrobial hydrogel [40] to bioinks for cell printing.…”

Section: −1mentioning

confidence: 69%

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Forget

Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

COMMUNICATION (1 of 7)© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Mechanically Tunable Bioink for 3D Bioprinting of Human CellsAurelien Forget, Andreas Blaeser, Florian Miessmer, Marius Köpf, Daniela F. Duarte Campos, Nicolas H. Voelcker, Anton Blencowe, Horst Fischer,* and V. Prasad Shastri* DOI: 10.1002/adhm.201700255 medium-the bioink. [5] The former approach offers the advantage that the 3D scaffold does not have to be fabricated under cytocompatible conditions. Therefore, a broader range of materials can be employed, for example, thermoplasts, such as poly(caprolactone), [6] and additionally, other alternative scaffold fabrication techniques, such as electrospinning, or pressurized gyration can be combined with subsequent functionalization of the scaffold with proteins. [7] In contrast, the latter approach, that is, direct fabrication of cellloaded constructs by hydrogel molding or 3D printing, places high demands on the cytocompatibility of the material and the fabrication process and the resolution is limited by the size of the extrusion nozzle utilized in the fabrication process. [8] However, the advantage of direct fabrication techniques, especially of 3D bioprinting, is its ability to generate constructs with spatially defined cell and material composition. Moreover, biomaterials that are conventionally used in cell culture such as alginate, [9] Pluronic, [10] gelatin, [11] nanocellulose, [12] self-assembling peptides, [13] and agarose [14] are highly advantageous for direct cell printing as they are soluble in water and hence can be formulated as a cell carrier. There has been extensive effort to build on and improve the properties of watersoluble polymers as bioinks. [15,16] For example, to overcome the limitation of the solubilization of Pluronic and its limited diversity of mechanical properties, blending of Pluronic with alginate [17] and crosslinking using acrylate-modified Pluronic have been explored. [10] Notwithstanding these advances that utilize chemical crosslinking to control the mechanical properties of the bioinks, controlling the shear behavior and mechanical This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5-230 Pa) while ensuring almost no change to the shear viscosity (10-17 mPa) of the bioink solution; thus enabling the fabrication of 3D structures made of different mechanical domains under identical printing parameters and low nozzle shear stress. Human mesenchymal stem cells printed using CA as a bioink show significantly higher survival (95%) in comparison to when printed using native agarose (62%), a commonly used thermogelling hydrogel for 3D-bioprinting applications. This work paves the way toward the printing of complex tissue-like structures composed of a range of mechanically discrete microdomains that could potent...

show abstract

Section: −1mentioning

confidence: 69%

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Forget

Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…The wound healing process is commonly accompanied by pH changes due to bacterial infection, inflammation and oxygenation. [ 75 ] These chemical changes in the environment have been exploited by pH‐responsive materials to tune delivery. Generally, pH responsive materials contain ionizable groups or acid‐cleavable bonds that are capable of releasing bioactive cargos in response to environmental pH changes.…”

Section: Stimuli‐responsive Growth Factor Delivery Systems In Tissue mentioning

confidence: 99%

Stimuli‐Responsive Delivery of Growth Factors for Tissue Engineering

Jiang

Zhou

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

Growth factors (GFs) play a crucial role in directing stem cell behavior and transmitting information between different cell populations for tissue regeneration. However, their utility as therapeutics is limited by their short half‐life within the physiological microenvironment and significant side effects caused by off‐target effects or improper dosage. “Smart” materials that can not only sustain therapeutic delivery over a treatment period but also facilitate on‐demand release upon activation are attracting significant interest in the field of GF delivery for tissue engineering. Three properties are essential in engineering these “smart” materials: 1) the cargo vehicle protects the encapsulated therapeutic; 2) release is targeted to the site of injury; 3) cargo release can be modulated by disease‐specific stimuli. The aim of this review is to summarize the current research on stimuli‐responsive materials as intelligent vehicles for controlled GF delivery; Five main subfields of tissue engineering are discussed: skin, bone and cartilage, muscle, blood vessel, and nerve. Challenges in achieving such “smart” materials and perspectives on future applications of stimuli‐responsive GF delivery for tissue regeneration are also discussed.

show abstract

“…Carboxylic acid groups have been incorporated into hydrogels, thus giving them capability of self‐healing, which is mediated by hydrogen bonding (Phadke et al, ). Acid‐functional hydrogels chelate various metal ions (Johnson, Komane, N'Da, & Neuse, ; Li, Zhao, Teasdale, John, & Zhang, ; Xu, ; Yetimoglu Kok, Kahraman, Ercan, Akdemir, & Kayaman Apohan, ); they are widely exploited for biomedical applications including tissue engineering and delivery of therapeutic agents (Cinay et al, ; Dadsetan et al, ; Kurkuri & Aminabhavi, ; Li et al, ; Ninan, Forget, Shastri, Voelcker, & Blencowe, ; Park, Nho, Lim, & Kim, ; Tasdelen, Kayaman‐Apohan, Guven, & Baysal, ; Tomic, Micic, Dobic, Filipovic, & Suljovrujic, ; Wu et al, ). Negatively ionizable acid groups are believed to facilitate the binding of Ca 2+ ions and to trigger mineralization; several studies have demonstrated that it is possible to mimic the process of bone formation by designing synthetic hydrogels decorated with carboxylate groups (Huang, Liu, Song, Saiz, & Tomsia, ; Chirila & Zainuddin, ; Filmona, Grizon, Basle, & Chappard, ; Grassmann & Lobmann, ; Kokubo & Takadama, ; Liu et al, ; Phadke, Zhang, Hwang, Vecchio, & Varghese, ; Song, Malathong, & Bertozzi, ).…”

Section: Introductionmentioning

confidence: 99%

Stimuli‐responsive poly(hydroxyethyl methacrylate) hydrogels from carboxylic acid‐functionalized crosslinkers

Bingol

Agopcan-Cinar

Bal

et al. 2019

J Biomedical Materials Res

View full text Add to dashboard Cite

Tailoring hydrogel properties by modifications of the crosslinker structure is a good method for the design of hydrogels with a wide range of properties. In this study, two novel carboxylic acid‐functionalized dimethacrylate crosslinkers (1a and 2a) are synthesized by the reaction of poly(ethylene glycol) or 2‐hydroxyethyl disulfide with tert‐butyl α‐bromomethacrylate followed by cleavage of tert‐butyl groups using trifluoroacetic acid. Their copolymerization reactivity with 2‐hydroxyethyl methacrylate (HEMA) investigated by photopolymerization studies performed on photo‐differential scanning calorimetry shows higher reactivity of 2a compared to 1a. These crosslinkers are then used at different ratios for fabrication of pH‐ and redox‐responsive poly(2‐hydroxyethyl methacrylate)‐based hydrogels. The swelling behavior of the hydrogels is found to be dependent on the structure of the crosslinker, degree of crosslinking, pH, and CaCl2 concentration. The redox‐responsive behavior is demonstrated by degradation of the hydrogel upon exposure to 1,4‐dithiothreitol. The dye Rhodamine 6G and the drug resorcinol are used as models to demonstrate the pH and redox dependent release of loaded compounds from the hydrogels. The electrostatic interactions between the carboxylate groups and the positively charged R6G are found to govern the release profile in DTT and counteract the diffusion of dye molecules and significant amount of release (79% in 120 hr) occurs only at highly acidic conditions. The degradation mediated release in DTT is observed better in case of resorcinol (around 88% in 5 hr). Overall, these hydrogels can be regarded as good candidates for several applications, such as matrices for controlled release, tissue repair, and regeneration.

show abstract

Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing

Cited by 492 publications

References 68 publications

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Stimuli‐Responsive Delivery of Growth Factors for Tissue Engineering

Stimuli‐responsive poly(hydroxyethyl methacrylate) hydrogels from carboxylic acid‐functionalized crosslinkers

Contact Info

Product

Resources

About