Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction

Abstract

Infection is the major cause of morbidity after breast implant surgery. Biodegradable medical-grade polycaprolactone (mPCL) scaffolds designed and rooted in evidence-based research offer a promising alternative to overcome the limitations of routinely used silicone implants for breast reconstruction. Nevertheless, as with any implant, biodegradable scaffolds are susceptible to bacterial infection too, especially as bacteria can rapidly colonize the biomaterial surface and form biofilms. Biofilm-related infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation of surrounding tissue. To date, no clinical solution that allows to efficiently prevent bacterial infection while promoting correct implant integration, has been developed. In this study, we demonstrated for the first time, to our knowledge that the physical immobilization of 1 and 5% human serum albumin (HSA) onto the surface of 3D printed macro- and microporous mPCL scaffolds, resulted in a reduction of Staphylococcus aureus colonization by 71.7 ± 13.6% and 54.3 ± 12.8%, respectively. Notably, when treatment of scaffolds with HSA was followed by tannic acid (TA) crosslinking/stabilization, uniform and stable coatings with improved antibacterial activity were obtained. The HSA/TA-coated scaffolds were shown to be stable when incubated at physiological conditions in cell culture media for 7 days. Moreover, they were capable of inhibiting the growth of S. aureus and Pseudomonas aeruginosa, two most commonly found bacteria in breast implant infections. Most importantly, 1%HSA/10%TA- and 5%HSA/1%TA-coated scaffolds were able to reduce S. aureus colonization on the mPCL surface, by 99.8 ± 0.1% and 98.8 ± 0.6%, respectively, in comparison to the non-coated control specimens. This system offers a new biomaterial strategy to effectively translate the prevention of biofilm-related infections on implant surfaces without relying on the use of prophylactic antibiotic treatment.

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

“…253 Crosslinking of a bioink by TA solution is more sensitive to the TA concentration, resulting in weak cell proliferation due to cell fixation by TA at higher 3 wt%. [254][255][256] Other polymers such as polycaprolactone (PCL) 257 and methylcellulose 258 3D printed hydrogels have been crosslinked similarly.…”

Insight on Serum Albumin: From Structure and Biological Properties to Functional Biomaterials for Bone Repair

“…aureus, E. coli, and so on. − As the albumin coating blocks bacterial adhesion and prevents the local environment from becoming acidic, it can also enhance the corrosion resistance of metallic implants.…”

Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis

“…Prior research indicates that breast implant bacterial contamination can manifest without clinical symptoms, leading to a chronic subclinical infection linked to capsular contracture in >10% of patients [ 41 , 44 , 45 ]. The routine use of antimicrobial pocket irrigation, implant-soaking agents, the inframammary fold incision technique, and submuscular implant placement has led to decreased rates of capsular contracture [ 46 , 47 , 48 , 49 , 50 , 51 ].…”