Antibacterial activity of three medicinal Thai plants againstCampylobacter jejuniand other foodborne pathogens

Abstract

Leaves of Adenanthera pavonina, Moringa oleifera and Annona squamosa are used in traditional Thai medicine to treat dysentery and other diseases. This study investigated the antibacterial activity of these plants against six species of foodborne pathogen. Methods and solvents employed to extract active constituents were optimised using the disc diffusion assay. Phytochemical analysis of the optimised extracts was performed by thin layer chromatography (TLC). Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined by broth microdilution. A. pavonina contained flavonoids, terpines and tannins, and was the most active extract against Campylobacter jejuni, inhibiting growth at 62.5-125 µg mL(-1). The A. squamosa extract contained flavonoids, terpines, tannins and alkaloids, and had the broadest spectrum of antibacterial activity, inhibiting Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and C. jejuni between 62.5 and 500 µg mL(-1). MBCs were 2- to 4-fold higher than MICs against C. jejuni and B. cereus, suggesting the extracts are bactericidal against these species. Negligible activity was detected from M. oleifera. The data presented here show that A. pavonina and A. squamosa could potentially be used in modern applications aimed at the treatment or prevention of foodborne diseases.

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

“…The crude extracts of Annona coriacea (species rich in aromatic acetogenins) have been observed to partially or totally disrupt midgut epithelial cells of A. aegypti [10]. However the use of crude extract does not indicate that the Annonaceae acetogenins cause these changes because plants of this family produce other secondary compounds toxic to insects, such as tannins, alkaloids and lectins [11]. …”

Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

“…Zeid et al (2012) reported its use as a tonic. These reports of traditional use were most common in Asian countries (Wickramaratne et al, 2016;Koodalingam et al, 2015;Dholvitayakhun et al, 2012;Arshad et al, 2010).…”

“…A study carried out by Dholvitayakhun et al (2012) investigated the antibacterial activity of aqueous, ethanolic, and hexane extracts of leaves of A. pavonina against foodborne pathogens. Initially, the efficacy of extraction methods was assessed using the disc diffusion assay against Campylobacter jejuni, and erythromycin discs (10 μg/disc) were used as a reference.…”

Evaluation of Adenanthera pavonina Bark Extracts for Antioxidant Activity and Cytotoxicity against Cancer Cell Lines

“…These phytochemicals are known to have many biological activities. In vitro studies show that Adenanthera pavonina leaf extract has antibacterial activity against the intestinal pathogen Campylobacter jejuni (Dholvitayakhun et al, 2012). Also, high doses of seed extract have an antiinflammatory effect in studies in rats and mice (Olajide, et al, 2004).…”