Antibacterial activity of temu kunci tuber (&lt;em&gt;kaempheria pandurata&lt;/em&gt;) essential oil against &lt;em&gt;Bacillus cereus&lt;/em&gt;

Miksusanti, Miksusanti; J, Betty S.L.; Syarief, Rizal; Pontjo, Bambang; Mulyadi, Gatot Tri

doi:10.13181/mji.v18i1.331

Cited by 9 publications

(9 citation statements)

References 16 publications

(10 reference statements)

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“…Bacterial pellets was prepared similarly as in the observation of the cell bacteria's protein profile. The morphology of cell pellets were observed using JEOL JSM-6360LA SEM (Scanning Electron Microscopy) as previously conducted by Miksusanti et al (2009).…”

Section: Observation Of B Cereus Cells' Morphology Transformationmentioning

confidence: 99%

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Asnani¹,

Rahayu²,

Jenie³

et al. 2018

MJM

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Aims: This study was conducted to observe the stability of Spondias pinnata leaf (SPL) extract antibacterial at different pH, salt concentration and temperature, to examine its antibacterial effectivity on minced fish, and to determine its fraction's antibacterial mechanism. Methodology and results: The tested SPL ethanolic extract, n-hexane, chloroform, ethyl acetate and water fractions' antibacterial activity against Bacillus cereus and Vibrio parahaemolitycus. Its stability against pH, salt, and thermal variation was studied, as well as the mechanism and application in fish. Ethyl acetate fraction and water fraction showed the highest activity against B. cereus (MIC 0.62 mg/mL). Protein profile analysis using gel electrophoresis showed that B. cereus cells exposed with SPL ethyl acetate fraction and water fraction showed thinner protein bands as compared to control. Severe damage of the cells treated with 3 MIC was also observed under SEM. Antibacterial activity of SPL ethanolic against Bacillus cereus and Vibrio parahaemolitycus were stable against heat treatment (80-121 °C for 15 min) and NaCl treatment (0-10% w/v), whereas the inhibition zone respectively at pH 4 (10.31±0.25 and 8.09±0.97 mm) was higher than pH 7 (8.45±0.52 and 6.66±1.84 mm). Application of SPL ethanolic extract in fish broth showed higher antibacterial activity than in fish flesh, which gave bactericidal effect at 3 MIC. Conclusion, significance and impact study: Ethanol extract can be developed as a natural preservative in fish processing.

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Section: Observation Of B Cereus Cells' Morphology Transformationmentioning

confidence: 99%

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Asnani¹,

Rahayu²,

Jenie³

et al. 2018

MJM

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“…This is because the three compounds contain aldehid groups. The aldehyde group in the volatile oil is a semi-polar compound which is hydrophilic [17,18].…”

Section: H Changes In Cell Morphology Bacillus Subtilismentioning

confidence: 99%

“…These components can interact with the cell membrane, where the component is dissolved in the phospholipid layer and bonded between the fatty acid chain. This process can cause membrane instability, increased membrane fluidity and alter membrane permeability [17,18] acids. The essential oil of lime leaves causes leakage of Ca 2+ and K + ions as shown in Figure 3.…”

Section: H Changes In Cell Morphology Bacillus Subtilismentioning

confidence: 99%

Activity Test and Inhibitory Mechanism of Essential Oil of Lime Leaf (Citrus Aurantifolia, Swingle) Against Some of Bacterial Pathogens

Musdja¹,

Pariwidjayanti²,

Agusta³

2018

Proceedings of the International Conference on Science and Technology (ICOSAT 2017)

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Lime fruit (Citrus aurantifolia Swingle) is traditionally known to be useful for the treatment of various infectious diseases like fever, cough, toothache, sore throat, diphtheria, body odor, ringworm etc as folk medicine. Objectives: This study aims to determine the activity and mechanism of inhibition of the growth of some bacterial pathogens by the essential oil of lime leaves (Citrus aurantifolia, swingle). Materials and Methods: The lime leaves was obtained from Balitro Bagor, distilled by steam distillation process. The essential oil of lime leaf that obtained was analyzed by Gas Chromatography-Mass Spectroscopy. The activity test of anti-bacterial of essential oil of lime leaves was carried out by using the method mikrodilution against the bacterium Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus and Bacillus subtilis. For detect the mechanism of cell damage process of bacteria was observed leakage of proteins and nucleic acids by using Ultra Violet-Visible spectrophotometer and the leakage of cations K + and Ca 2+ was observed by using Atomic Absosrtion Spectrum. While bacterial cell morphology change was observed by using Scanning Electron Microscope (SEM). Results and Conclusion: From the research was obtained, Lime leaf contains about 0.4% (v/w) essential oils with the main components are geranial (10.39%), limonene (10.2%), neral (8.94%), kariofilena (5.72%) and citronellal (5.41%). The essential oil of lime leaf has the highest antibacterial activity on the bacteria tested was on Bacillus subtilis, with a minimum inhibitory concentration (MIC) of 0.125% (v/v). Test results nucleic acid and protein leakage and cations leakage of K + and Ca 2+ greatly increased from 1 MIC to 2 MIC concentration of the tested bacteria compared with the normal controls. While the observations that was detected by using Scanning Electron Microscope (SEM) showed worse cell damage by giving the higher doses to bacteria.

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“…Kerusakan dinding sel bakteri telah diamati oleh peneliti terdahulu, diantaranya menguji aktivitas antibakteri daun dan umbi Crinum asiaticum Ldan monoasil gliserol minyak kelapa, daun sirih dan temu kunci (kaempheria pandurata). 6,7,8,9 Penelitian ini bertujuan untuk mengetahui mekanisme kerja antibakteri kulit batang jambu mete dengan berbagai konsentrasi terhadap S.aureus dengan mengukur jumlah Ca 2+ dan K + menggunakan AAS (Atomic Absorption Spectroscopy) Perkin Elmer. Pemberian ekstrak etanolik kulit batang jambu mete pada konsentrasi 3%, 5% dan 7% dapat mengakibatkan keluarnya ion logam dari sel bakteri, antara lain ion kalium dan kalsium.…”

Section: Pendahuluanunclassified

Aktivitas antibakteri ekstrak etanolik kulit batang jambu mete (Anacardium occidentale Linn.) terhadap Staphylococcus aureus

Harsini

2017

Majalah Kedokteran Gigi Indonesia

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Antibacterial activity of cashew stembark (Anacardium occidentale Linn) on Staphylococcus aureus. Microbial activity acts as a sign of disruption of bacterial growth. The bark of cashew (Anacardium occidentale Linn.) contains phenolic compounds such as flavonoids, tannins and anacardic acid which have an activity as antimicrobial. One of the Gram positivebacteria in the oral cavity was Staphylococcus aureus (S. aureus). The aim of the study was to observe the bacterial activity of ethanolic extract of the cashew to a metal ion, i.e. Ca2+ and K+ leakage from S aureus. This research used one ose bacteria S. aureus at a density of 106 standard Brown as much as 10 mL and centrifuged at a speed of 3500 rpm for 20 minutes. The filtrate discarded, pellets in the tube was washed using phosphate buffer pH 7.0. Furthermore the ethanolic extract of the bark of the cashew stembark was added in the concentrations of 3.5% and 7% without any extract as a control, each of which was in 5 tubes, incubated in an incubator for 24 hours. The suspension was centrifused with a speed of 3500 rpm for 20 minutes prior to be filtered. Supernatant liquid was taken and measured absorbance using AAS. Data were analyzed using one way Anova p = 0.05. The results showed that leakage of Ca2+ was at concentrations of 0%, 3%, 5% and 7% were 2.42 ± 0.82; 32.87 ± 1.97; 49.10 ± 3.33; 66.73 ± 3.29, respectively while for the K+ metal was 15.28 ± 0.46; 606.36 ± 14.14; 895 ± 9.5; 1251 ± 11.54. Anova one way showed a significant effect (p <0.050) ethanolic extract of the bark of cashew against leakage of metal ions Ca2+ and K+ at S aureus bacteria. LSD test showed a significant difference among all treatment groups. It was concluded that there was antibacterial activity of ethanolic extract of the cashew stembark on bacteria S. aureus based on leakage of metal ions Ca2+ and K+ The highest leakage of metal ions was at the concentrations of 7%. ABSTRAKAktivitas antibakteri merupakan tanda terganggunya pertumbuhan bakteri. Kulit batang tanaman jambu mete (Anacardium occidentale Linn.) mengandung senyawa fenolik seperti flavonoid dan tanin serta asam anakardat yang memiliki aktivitas sebagai antibakteri. Salah satu bakteri gram positif dalam mulut yang patogen adalah Staphylococcus aureus (S.aureus). Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit batang jambu mete (anacardium occidentale Linn.) terhadap S.aureus yang ditandai dengan kebocoran ion logam. Penelitian menggunakan sebanyak 1 ose dengan kepadatan 106 CFL/mL disentrifuse dengan kecepatan 3500 rpm selama 20 menit. Filtrat dibuang, pellet dalam tabung dicuci menggunakan buffer fosfat pH 7,0. Ekstrak etanolik kulit batang jambu mete konsentrasi 3; 5 dan 7% serta tanpa ekstrak sebagai kontrol, masing-masing dalam 5 tabung, diinkubasi dalam inkubator goyang selama 24 jam. Suspensi kemudian disentrifuse dengan kecepatan 3500 rpm selama 20 menit lalu disaring. Cairan supernatan diambil diukur absorbansinya menggunakan AAS (Atomic absorption Spectroscopy). Data dianalisis menggunakan Anava satu jalur. Hasil menunjukkan kebocoran Ca2+ pada konsentrasi 0, 3, 5 dan 7% berturut-turut adalah 2,42 ± 0,82; 32,87 ± 1,97; 49,10 ± 3,3; 66,73 ± 3,29, sedangkan logam K+ adalah 15,28 ± 0,46; 606,36 ± 14,14; 895 ± 9,5; 1251 ± 11,54. Hasil analisis statistik Anava menunjukkan terdapat aktivitas antibakteri ekstrak etanolik kulit batang jambu mete. Hasil LSD menunjukkan terdapat perbedaan yang bermakna antar seluruh kelompok perlakuan. Kesimpulan terdapat aktivitas antibakteri ekstrak etanolik kulit batang jambu mete terhadap S.aureus dilihat dari kebocoran ion logam Ca2+ and K+. Kebocoran tertinggi pada konsentrasi ekstrak 7%.

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Antibacterial activity of temu kunci tuber (<em>kaempheria pandurata</em>) essential oil against <em>Bacillus cereus</em>

Cited by 9 publications

References 16 publications

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Activity Test and Inhibitory Mechanism of Essential Oil of Lime Leaf (Citrus Aurantifolia, Swingle) Against Some of Bacterial Pathogens

Aktivitas antibakteri ekstrak etanolik kulit batang jambu mete (Anacardium occidentale Linn.) terhadap Staphylococcus aureus

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