Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Abstract

Aims: This study was conducted to observe the stability of Spondias pinnata leaf (SPL) extract antibacterial at different pH, salt concentration and temperature, to examine its antibacterial effectivity on minced fish, and to determine its fraction's antibacterial mechanism. Methodology and results: The tested SPL ethanolic extract, n-hexane, chloroform, ethyl acetate and water fractions' antibacterial activity against Bacillus cereus and Vibrio parahaemolitycus. Its stability against pH, salt, and thermal variation was studied, as well as the mechanism and application in fish. Ethyl acetate fraction and water fraction showed the highest activity against B. cereus (MIC 0.62 mg/mL). Protein profile analysis using gel electrophoresis showed that B. cereus cells exposed with SPL ethyl acetate fraction and water fraction showed thinner protein bands as compared to control. Severe damage of the cells treated with 3 MIC was also observed under SEM. Antibacterial activity of SPL ethanolic against Bacillus cereus and Vibrio parahaemolitycus were stable against heat treatment (80-121 °C for 15 min) and NaCl treatment (0-10% w/v), whereas the inhibition zone respectively at pH 4 (10.31±0.25 and 8.09±0.97 mm) was higher than pH 7 (8.45±0.52 and 6.66±1.84 mm). Application of SPL ethanolic extract in fish broth showed higher antibacterial activity than in fish flesh, which gave bactericidal effect at 3 MIC. Conclusion, significance and impact study: Ethanol extract can be developed as a natural preservative in fish processing.

Biological Activity of Lasiodiplodia pseudotheobromae IBRL OS- 64 Extracts, an Endophytic Fungus Isolated from Medicinal Herb, Ocimum sanctum Against Foodborne Diarrhea-Caused Bacteria

“…As for Gram-positive bacteria, some cavities and dents were observed on the treated cell. A similar observation reported by Asnani 46 who studied the morphology of B. cereus cells exposed to water and ethyl acetate fraction of Spondias pinnata leaves under SEM.…”