Antibacterial activity of silver nanoparticles against methicillin-resistant Staphylococcus aureus synthesized using model Streptomyces sp. pigment by photo-irradiation method
In this work, the antimicrobial property of nanocomposite prepared by loading of silver nanoparticle onto activated carbon (AgNP-AC) was studied. The silver nanoparticles were synthesized biologically from the culture Enterobacter aerogenes and loaded onto the activated carbon. The nanoparticle and nanocomposite were characterized by UV-vis, scanning electron microscopy and X-ray diffraction studies. The spectroscopic assay and zone inhibition test for E. coli and B. subtilis clearly illustrates the antibacterial activity of the silver nanoparticle and nanocomposite. The antimicrobial activity of the nanocomposite was of the nanocomposite was also confirmed with the observed zone of inhibition against beverage, brewery, dairy and dye industrial effluents.
In this work, the antimicrobial property of nanocomposite prepared by loading of silver nanoparticle onto activated carbon (AgNP-AC) was studied. The silver nanoparticles were synthesized biologically from the culture Enterobacter aerogenes and loaded onto the activated carbon. The nanoparticle and nanocomposite were characterized by UV-vis, scanning electron microscopy and X-ray diffraction studies. The spectroscopic assay and zone inhibition test for E. coli and B. subtilis clearly illustrates the antibacterial activity of the silver nanoparticle and nanocomposite. The antimicrobial activity of the nanocomposite was of the nanocomposite was also confirmed with the observed zone of inhibition against beverage, brewery, dairy and dye industrial effluents.
Diseases caused by Staphylococcus warneri have a significant impact on human health. We evaluated the antibacterial activity of silver nanoparticles (synthesized using the endophytic strain SYSU 333150) against S. warneri. The strain SYSU 333150 was isolated from the roots of Borszczowia aralocaspica Bunge. The 16S rRNA sequence results suggest that SYSU 333150 belongs to the genus Isoptericola and is likely a new species. Photo-irradiation was used to synthesize silver nanoparticles, which were characterized using UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction. The nanoparticles were spherical and measured to be11 to 40 nm. X-ray diffraction revealed four peaks corresponding to the 111, 200, 220, and 311 planes of the face-centered cubic lattice, indicating a crystalline nature. Fourier transform infrared spectroscopy suggested that the metabolites in the culture supernatant were likely reducing and capping agents. The silver nanoparticles possessed antimicrobial activity (14 mm zone of inhibition) against S. warneri, which was likely a result of DNA cleavage. The synthesized silver nanoparticles have potent antibacterial activity against S. warneri and can be used to control infection.
“…One promising alternative which has been proposed is nanosilver [23]. Metallic nanoparticles in general have been reported to have broad spectrum activity against both Gram-positive and Gram-negative bacteria including antibiotic resistant bacteria like MRSA and MRSE [2,18,20].…”
a b s t r a c tHypothesis: Metallic nanoparticles such as nano-silver have found many applications as alternative antimicrobials in recent years. However methods for determining their proposed antimicrobial activity have received little attention to date. The disk diffusion assay is commonly used as a demonstration of antimicrobial properties and is a regular feature in synthetic nanoparticle papers. The aim of this study was to assess its effectiveness in demonstrating the ''nanoparticle specific'' antimicrobial properties in the absence of ionic contributions from unreacted reducing agents and or impurities. Experiments: The disk diffusion assay was carried out on a range of silver nanoparticles, both in-house synthesised and commercially available, using Escherichia coli ATCC 25922 as a model organism. Results: Capped and purified nanoparticles show no antimicrobial activity despite claims to the contrary for this assay. Results will be discussed in terms of the need for researchers without a background in microbiology to understand the mechanism of antimicrobial action before choosing an assay. Also discussed is the importance understanding the physiochemical characteristics of when interpreting results. Finally the relevance of the results in terms establishing protocols for method development for 'nanoparticle specific' antimicrobial properties will also be considered.
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