Antibacterial Activity of Scopoletin from Stem Bark of Aleurites moluccana Against Salmonella typhi

Abstract

Background: Based on a previous survey of ethnopharmaceutical studies (local culture), it was known that the use of decoction of candlenut stem bark (Aleurites Moluccana) was used as typhoid medicine in the treatment tradition of the people of East Kalimantan. In antibacterial screening by diffusion test, methanol extract of stem bark A.moluccana showed excellent growth inhibition of Salmonella thypimurium. Working method: Simplicia of A.moluccana stem bark was extracted by maceration using methanol. Maserate was extracted liquid-liquid using n-hexane and ethyl acetate solvents. The hexane and ethyl acetate fraction was monitored for active spots with TLC bioautography. The active compound is separated using vacuum liquid chromatography and radial chromatography. Results: The fraction J of KCV and J2 subfraction results from radial chromatography provides antibacterial activity of S. thpimurium. From the J2 subfraction obtained pure isolates in the form of yellowish needle crystals. The isolate was tested for antibacterial S.thypimurium using the microdilution method with a value of MIC is 250 µg/ml. Based on spectroscopic data and comparing the published spectra of the compound, the elucidation of the isolate is Scopoletin (7-hydroxy-6-methoxycoumarin).

Identification of natural small molecule modulators of MurB from Salmonella enterica serovar Typhi Ty2 strain using computational and biophysical approaches

“…Literature was explored for the presence of plant‐based small molecule natural compounds with demonstrated experimental binding and inhibitory activity against various bacterial and fungal species to include Mur ligases from different bacterial organisms 45–50 . A total of 50 compounds were selected, including those from terpenoids, alkaloids, coumarins, flavonoids, and other classes of plant phytochemicals 51–60 . The prepared 3D structures of these compounds were then virtually screened against the prepared stMurB model utilizing Virtual Screening protocol of Maestro.…”

“…[45][46][47][48][49][50] A total of 50 compounds were selected, including those from terpenoids, alkaloids, coumarins, flavonoids, and other classes of plant phytochemicals. [51][52][53][54][55][56][57][58][59][60] The prepared 3D structures of these compounds were then virtually screened against the prepared stMurB model utilizing Virtual Screening protocol of Maestro. This filtered out top three hits, quercetin, berberine, and scopoletin, which were then considered for subsequent in silico and in vitro analysis.…”

Phytochemistry and Biological-Pharmacological Profile of Aleurites moluccanus: A Critical Review

“…Using UV-Vis spectrophotometer and NMR. Two compounds were isolated from the meth- anolic extract from stem bark of A. moluccanus, the coumarin scopoletin and the pentacyclic triterpene 3-acetyl aleuritolic [28]. The benzene extract of the heartwood of A. moluccanus yielded a novel phorbol diester, 13-O-myristyl-20-O-acetyl-12-deoxyphorbol.…”

Aleurites moluccana (L.) Willd. Euphorbiaceae

“…Bark extracts also exhibit antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pyogenes (Abd and Mohamad 2010). Scopoletin isolated from the barks could inhibit the growth of Salmonella typhimurium (Prabowo and Agustina 2020). Husk extract has antibacterial activity against Klebsiella pneumoniae (Abd and Mohamad 2010).…”