Antibacterial activity of proteins extracted from the pulp of wild edible fruit of <i>Bromelia pinguin</i> L.

Ruíz-Ruíz, Jorge Carlos; Ramón-Sierra, Jesús; Arias-Argaez, Carolina; Magaña-Ortíz, Denis; Ortiz-Vázquez, Elizabeth

doi:10.1080/10942912.2016.1154572

Cited by 17 publications

(12 citation statements)

References 28 publications

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“…Antibacterial activity of peak F-3 (less AMPs but more other proteins) was higher against S. aureus and P. aeruginosa than E. coli, the same results were observed in our study, where anti-bacterial activities of WPs was higher against S. aureus, P. aeruginosa compared to E. coli. (Ruiz-Ruiz et al, 2017) also observed the same pattern with AMPs isolated from fruits of Bromelia pinguin L. They observed that activity of crude soluble proteins fraction was more against S. aureus (13.0 ± 0.40) than E. coli (12.0 ± 0.30). A reverse pattern was observed when puri ied protein fractions of the same were tested, where ZOI against E. coli was 8.0 ± 0.50mm, while no zone was observed against S. aureus.…”

Section: Antibacterial Activity Of Whole Proteinssupporting

confidence: 63%

Antibacterial potential of Antimicrobial peptides containing whole proteins of two Adiantum species from Himalaya against selected human bacterial pathogens

Negi¹,

Maurya²

2020

ijrps

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Increasing tendency of drug resistance and continuously emerging new diseases have limited the available therapeutic options. Plants are still a positive hope for search of new therapeutic agents, which have provided many effective therapeutic agents and have not been yet explored to their full potential. In the present study, two ferns Adiantum edgeworthii and Adiantum capilllus-veneris from the Himalayas, have been explored for the presence of antimicrobial peptides (AMPs) in them. Antimicrobial peptides are different class of therapeutic agents having multimodal action mechanism and different site of action. Hence they could be a solution against the problem of drug resistance. In the present study, whole proteins (WPs) of the ferns were precipitated using TCA, and low molecular weight protein fractions (LMPFs) of WPs were isolated using SDS-PAGE. Antibacterial activities of WPs and LMPFs were tested against six human pathogenic bacteria S. aureus, S. pneumoniae, E. faecalis, and K. pneumoniae, P. aeruginosa and E. coli. The results showed that AMPs were present both the ferns and antibacterial potency of LMPFs was higher than WPs. It was also observed that WPs of A. capillus-veneris was about 11.4% more active than WPs of A. edgeworthii. Antibacterial activity of WPs was compared with two standard antibiotics (Amoxicillin and Erythromycin) and comparable results were obtained. Further research for purification and characterization of pure antimicrobial peptides from these plants are expected to provide new therapeutic agents.

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Section: Antibacterial Activity Of Whole Proteinssupporting

confidence: 63%

Antibacterial potential of Antimicrobial peptides containing whole proteins of two Adiantum species from Himalaya against selected human bacterial pathogens

Negi¹,

Maurya²

2020

ijrps

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“…As demonstrated by Ruiz-Ruiz et al [ 34 ], the inhibition zones of 14.0–19.0 mm and >19.0 mm can be classified as active and very active, respectively. Thus, Z. spinosa has promising antibacterial activity.…”

Section: Discussionmentioning

confidence: 98%

Antimicrobial and Antioxidant Activities of Different Extracts from Different Parts of Zilla spinosa (L.) Prantl

Suleiman

Ateeg

2020

Evidence-Based Complementary and Alternative Medicine

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Zilla spinosa is commonly used in traditional medicine to treat gastrointestinal disorders and diabetes. In this study, aqueous ethanol (AE) and aqueous methanol (AM) extracts from aerial parts and roots of Z. spinosa were investigated. The total phenolic (TPC) and flavonoid (TFC) contents and antioxidant capacities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were determined, and the correlations among the results were assessed using Pearson’s correlation. The antimicrobial activity was assessed through agar disc diffusion and broth microdilution assays. Phytochemical screening showed that Z. spinosa extracts had alkaloids, glycosides, saponins, triterpenoids, phenols, and flavonoids in different abundances. The aerial part-AE extract contained low TPC (30.17 ± 4.24 mg GAE/g) and TFC (7.40 ± 1.02 mg QE/g) and displayed significant antioxidant capacity in the DPPH (IC50 = 52.17 ± 7.30 μg/mL), H2O2 (91.22 ± 2.60 μg/mL), and FRAP (EC50 = 98.70 ± 2.21 μg/mL) assays. By contrast, the root-AM extract contained high amounts of TPC (87.72 ± 7.75 mg GAE/g) and TFC (25.60 ± 1.57 mg QE/g). It showed significantly high antioxidant activity with IC50 values of 12.33 ± 1.88 μg/mL in the DPPH and 39.37 ± 2.59 μg/mL in the H2O2 assays, as well as reducing power capacity with an EC50 value of 20.82 ± 1.14 μg/mL in the FRAP assay. Both TPC and TFC were exhibited negative correlations ( p < 0.01 ) with the IC50 and EC50 values obtained in the applied antioxidant assays. The aerial part-AM extract showed the highest inhibitory activity against Staphylococcus aureus (26.5 ± 0.20 mm), followed by Shigella flexneri (19.4 ± 0.40 mm) and Proteus mirabilis (17.7 ± 0.49 mm). S. aureus was the most affected microorganism with a minimum inhibitory concentration of 128 μg/mL against the aerial part-AM extract. Interestingly, all evaluated extracts showed potent antifungal activity against Candida albicans. However, aerial part-AM was the most effective, with an inhibition zone of 12.6 ± 0.17 mm. The results concluded that Z. spinosa possesses different phytochemicals displaying significant antioxidant and antimicrobial activities, thus lending credence to its use in traditional medicine.

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“…Other species of Bromeliaceae also contain enzymes with proteolytic and antibacterial activities, such as the reported for Bromelia pinguin fruits, which displayed activity against E. coli and S. aureus (Payrol et al 2005;Carlos Ruiz-Ruiz et al 2017;Moreno-Hernández et al 2017).…”

Section: Introductionmentioning

confidence: 97%

Protein extract of Bromelia karatas L. rich in cysteine proteases (ananain- and bromelain-like) has antibacterial activity against foodborne pathogens Listeria monocytogenes and Salmonella Typhimurium

et al. 2021

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Bromelia karatas L. is a plant species from the Americas. The presence of proteases in fruits of B. karatas has been reported but scarcely studied in detail. Proteolytic enzymes from Ananas comosus have displayed antifungal and antibacterial activity. Thus, novel proteases present in B. karatas may be useful as a source of compounds against microorganisms in medicine and food production. In this work, the protein extract from the fruits of B. karatas was characterized and its antibacterial activity against Salmonella Typhimurium and Listeria monocytogenes was determined for the first time. Proteins highly similar to ananain and the fruit bromelain from A. comosus were identified as the main proteases in B. karatas fruits using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The soluble protein extract (SPE) at a concentration of 2.0 mg/ mL displayed up to 80% of antibacterial activity against S. Typhimurium. Complete inhibition of L. monocytogenes was reached with up to 1.65 mg/mL of SPE. Plant protease extract containing ananain-like enzyme inhibited up to 90% against S. Typhimurium and up to 85% against L. monocytogenes using only 10 μg/mL of the partial-purified enzyme.

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Antibacterial activity of proteins extracted from the pulp of wild edible fruit of Bromelia pinguin L.

Cited by 17 publications

References 28 publications

Antibacterial potential of Antimicrobial peptides containing whole proteins of two Adiantum species from Himalaya against selected human bacterial pathogens

Antibacterial potential of Antimicrobial peptides containing whole proteins of two Adiantum species from Himalaya against selected human bacterial pathogens

Antimicrobial and Antioxidant Activities of Different Extracts from Different Parts of Zilla spinosa (L.) Prantl

Protein extract of Bromelia karatas L. rich in cysteine proteases (ananain- and bromelain-like) has antibacterial activity against foodborne pathogens Listeria monocytogenes and Salmonella Typhimurium

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