Antibacterial Activity of Pomegranate (Punica granatum L.) Seed and Peel Alcoholic Extracts on Staphylococcus aureus and Pseudomonas aeruginosa Isolated From Health Centers

Nozohour, Yaser; Golmohammadi, Reza; Mirnejad, Reza; Fartashvand, M

doi:10.29252/jabr.01.01.06

Cited by 29 publications

(16 citation statements)

References 27 publications

(33 reference statements)

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“…The antimicrobial effect of ethanolic and methanolic pomegranate peel extracts reported in this study was previously reported by several authors [28,[36][37][38][39]. With respect to G + bacteria, our study is in line with others that reported the superiority of the methanolic pomegranate peel extract over the ethanolic one [36][37][38][39].…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Results The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains revealed that both pomegranate peel extracts exhibited significantly higher inhibitory effect on all tested G+ bacteria. Methanolic extract of pomegranate peel gave higher activity than the ethanolic one against G+ and G− bacteria except for S. typhimurium. Against A. flavus and A. niger, both pomegranate and orange extracts showed activity ranging between 65 and 100% more than the positive control. The ethanolic extracts of all tested peels showed a considerable capacity of antioxidant compounds compared to the methanolic extracts. The highest antioxidant capacity was found for ethanolic and methanolic extracts of pomegranate, 66.870 and 56.262 mg/ml, respectively. Generally, the concentration of total phenolic compounds was higher than that of total flavonoids followed by tannins. The highest readings of all tested constituents were reported for pomegranate extracts followed by orange and then banana. The total phenolic content, total flavonoids, and tannins were proportional to antioxidant values. GC-MS of pomegranate peel extracts identified 23 compounds in the methanolic extract versus 31 compounds in the ethanolic one. These components were identified based on their retention times and mass spectral fragmentation pattern. 5-hydroxymethylfufural (HMF) represented the major component in both methanolic and ethanolic extracts with peak area percentage of 65.78% and 48.43%, respectively. Conclusions The results showed negative effect of methanolic and ethanolic extracts of pomegranate on G+ and G− bacteria and two fungal pathogenic strains. The phytochemical analysis regarded these results to the high content of phenols, flavonoids, and tannins. GC-MS chromatogram identified many compounds known to be effective as antioxidants and antibacterial and antifungal agents. These indications show that pomegranate peel may be a superior natural food-preserver, but further studies about the suitable formulation, dosage, and possible side-effects are still needed.

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Section: Discussionsupporting

confidence: 92%

“…Concerning G − bacteria, previous studies reported the activity of methanolic, ethanolic, and aqueous extract of pomegranate peel, with even better results than these of the standard antibiotic [28,[38][39][40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…% (Figure 4). These results are clearly indicative of the high antimicrobial performance of pomegranate seed and peel extracts, as reported by several authors [57,58]. This behaviour can be related to the polyphenolic compounds present in PSP in accordance with previous results linking the antibacterial activity of pomegranate fruit and peel extracts with their total polyphenols content [59][60][61], being phenols, tannins and flavonoids the major active compounds responsible for this activity.…”

Section: Antimicrobial Activity Of Fg/psp Filmssupporting

confidence: 91%

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

et al. 2019

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Pomegranate (Punica granatum L.) seed juice by-product (PSP) was added as reinforcing and antimicrobial agent to fish gelatin (FG) films as a promising eco-friendly active material for food packaging applications. A complete linkage analysis of polysaccharides in PSP showed xylan and cellulose as main components. This residue showed also high total phenolic content and antioxidant activity. Three formulations were processed by adding PSP to FG (0, 10, 30 wt. %) by the casting technique, showing films with 10 wt. % of PSP the best performance. The addition of PSP decreased elongation at break and increased stiffness in the FG films, particularly for 30 wt. % loading. A good compatibility between FG and PSP was observed by SEM. No significant (p < 0.05) differences were obtained for barrier properties to oxygen and water vapour permeability compared to the control with the incorporation of PSP, whereas water resistance considerably increased and transparency values decreased (p < 0.05). High thermal stability of films and inhibition against S. aureus were observed. The addition of PSP at 10 wt. % into FG was shown as a potential strategy to maintain the integrity of the material and protect food against lipid oxidation, reducing huge amounts of pomegranate and fish wastes.

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“…Antimicrobial activity of many extracts from the different fragments of pomegranate shrub was previously premeditate, and numerous results were found by scientists [3]. In a study conducted by Nozohour et al [16], the ethanolic extracts showed inhibitory effects on clinical isolates of P. aeruginosa and S. aureus clinical isolates. The pomegranate peel and seed extracts had minimum inhibitory concentrations of 12.5 and 25.0 mg|ml,respectively [16].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Potential of Pomegranate Peel Extracts on Escherichia coli Isolated From Benha Hospital in Egypt

Abdel-Aziz

El-Esawi

Hazaa

et al. 2021

Benha Journal of Applied Sciences

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Introduction. Pomegranate is an old fruit which has many antimicrobial compounds. Impact of pomegranate peel extracts on Escherichia coli clinical isolate from Benha general hospital was investigated in this study. Material and Methods: pomegranate peel extraction was primed and GC/MS chromatogram analysis was carried out using the GC Agilent Technologies gas chromatography fitted with HP-5 fused silica column (25mm × 0.25mm, film thickness of 0.25 µm) and interfaced to the flame-ionization detector (FID). The antibacterial potential of Pomegranate peel extractions against E.coli sample was done using well diffusion method. Results: According to (GC/MS) technique, methanol and aqueous extracts of pomegranate peel have inhibitory effects on E. coli isolate. The minimum inhibition concentrations (MICs) of methanolic and aqueous extracts were 100 and 60 mg/L, respectively. In this study , the MICs values of Aqueous extract of pomegranate peel were higher than of methanolic extract of pomegranate peel (P<0.05). Zones of inhibition and values of MICs indicated that the aqueous extract of pomegranate peel had more effect on E. coli isolate than methanolic extract of pomegranate peel. Conclusion: In this study, the extraction of pomegranate peel has antibacterial effect on E.coli isolate. However, the aqueous extract had a higher effect on E.coli than the methanolic extract.

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Antibacterial Activity of Pomegranate (Punica granatum L.) Seed and Peel Alcoholic Extracts on Staphylococcus aureus and Pseudomonas aeruginosa Isolated From Health Centers

Cited by 29 publications

References 27 publications

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

Antibacterial Potential of Pomegranate Peel Extracts on Escherichia coli Isolated From Benha Hospital in Egypt

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