Antibacterial activity of Pinus elliottii against anaerobic bacteria present in primary endodontic infections

Silva, Sandro Donizete Caetano da; Souza, Maria Gorete Mendes de; Cardoso, Miguel Jorge Oliveira; Moraes, Thaís da Silva; Ambrósio, Sérgio Ricardo; Veneziani, Rodrigo Cássio Sola; Martins, Carlos Henrique Gomes

doi:10.1016/j.anaerobe.2014.09.013

Cited by 25 publications

(20 citation statements)

References 37 publications

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“…6 Both 1 and 1a themselves have an intrinsic activity against (mainly Gram-positive) bacteria and fungi. 6,7 The effort to enhance these activities has led to the synthesis of a multitude of diverse derivatives with reported antimicrobial activities, e.g. against Staphylococcus, Pseudomonas or Cladosporium sp.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial profiling of abietane-type diterpenoids

Helfenstein

Vahermo

Nawrot

et al. 2017

Bioorganic & Medicinal Chemistry

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Abietic and dehydroabietic acid are interesting diterpenes with a highly diverse repertoire of associated bioactivities. They have, among others, shown antibacterial and antifungal activity, potentially valuable in the struggle against the increasing antimicrobial resistance and imminent antibiotic shortage. In this paper, we describe the synthesis of a set of 9 abietic and dehydroabietic acid derivatives containing amino acid side chains and their in vitro antimicrobial profiling against a panel of human pathogenic microbial strains. Furthermore, their in vitro cytotoxicity against mammalian cells was evaluated. The experimental results showed that the most promising compound was 10 [methyl N-(abiet-8,11,13-trien-18-yl)-d-serinate], with an MIC of 60μg/mL against Staphylococcus aureus ATCC 25923, and 8μg/mL against methicillin-resistant S. aureus, Staphylococcus epidermidis and Streptococcus mitis. The IC value for compound 10 against Balb/c 3T3 cells was 45μg/mL.

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Section: Introductionmentioning

confidence: 99%

Antibacterial profiling of abietane-type diterpenoids

Helfenstein

Vahermo

Nawrot

et al. 2017

Bioorganic & Medicinal Chemistry

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“…In our study, a standardized extract containing about 48% of another diterpene (KA), showed to be able to reduce S. mutans biofilm at sub-MIC concentrations. In fact, recent studies have been demonstrated that diterpenes are very effective to inhibit the biofilm formed by different microorganisms, including anaerobic bacteria present in primary endodontic infections [16,44]. All these facts reinforce the relevance of this class of natural products to be considered in the search for novel oral care products.…”

Section: Resultsmentioning

confidence: 81%

“…In recent years, our research group has pointed out the antimicrobial potential of some subclasses of these metabolites against several oral pathogens: pimaranes [9,10,11], kauranes [12,13], labdanes [5,14,15], and abietanes [16]. Among all the investigated compounds, ent-kaurenoic acid (KA; Fig.…”

Section: Introductionmentioning

confidence: 99%

ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

et al. 2016

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Many studies have reported that medicinal plant extracts can inhibit oral pathogen growth or adhesion to surfaces and therefore reduce dental caries formation. The addition of these extracts to oral products like mouthwashes and dentifrices is considered an important strategy in caries control. In this sense, we have developed a Mikania glomerata extract with high ent-kaurenoic acid content (KAMg). So, this work describes the preparation of such extract and the development of a validated HPLC-DAD method to determine its ent-kaurenoic acid (KA) content. Herein it is also described the KAMg in vitro antibacterial evaluation against several cariogenic bacteria in comparison with KA and the investigation of further aspects of the KAMg activity. Toxicological aspects of the developed extract were evaluated by assessing its cytotoxicity and genotoxicity. KA and a KA-rich extract like KAMg showed to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC (Minimum inhibitory concentration) values, albeit not as low as the MIC value obtained for chlorhexidine digluconate (CHD), the golden anticariogenic standard approved by the American Dental Association Council on Dental Therapeutics. However, KAMg was more effective to inhibit the formation of a Streptococcus mutans biofilm with four times lower MICB50 (minimum inhibitory concentration that reduces 50% of the biofilm) value as compared with CHD. Taking into account all these data and considering the absence of genotoxic and cytotoxic activity under the tested conditions, it is suggested that KAMg is a natural product to be considered as active ingredient in oral care products.

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“…The antibiofilm activity was also measured by counting the number of microorganisms. The procedures were adapted according to the methodology described by Caetano da Silva et al () with some modifications regarding culture medium, incubation time and temperature, but they were performed on another microplate. The experiments were carried out in triplicate for all the assayed EOs.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of essential oils for potential antibacterial effects against Xylella fastidiosa

Santiago

Moraes

Massuco

et al. 2018

Journal of Phytopathology

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The main pathogen underlying citrus variegated chlorosis (CVC), a citrus fruit disease, is Xylella fastidiosa, a Gram‐negative s‐shaped bacterium that blocks water and nutrient circulation. Discovering new pesticides to combat CVC have become a challenge. Research into essential oils (EOs) is crucial because these substances can be potentially useful in pest control. This study aims to determine minimum inhibitory concentrations (MIC), to investigate biofilm inhibition capacities through MBIC50 analysis, and to evaluate synergistic effects with streptomycin of 17 EOs against X. fastidiosa. This study also analyses the chemical profiles of the EOs that provided the best antibacterial activity results. The MIC values of the EOs ranged from 125 to 1,000 µg/ml. Analysis of the MICB50 values of the sandalwood and patchouli EOs revealed biofilm inhibitory activity. No synergistic effects were evident for streptomycin combined with the sandalwood or patchouli EOs: ΣFIC was 1.25 and 1.5, respectively, which denoted indifference. We detected α‐gurjunene (27.6%), patchouli alcohol (21.6%), α‐bulsene (17.3%) and α‐patchoulene (6.3%) as the major patchouli EO constituents. On the basis of our results, the sandalwood and patchouli EOs present antibacterial action and are promising natural sources for the development of new pesticides against X. fastidiosa.

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Antibacterial activity of Pinus elliottii against anaerobic bacteria present in primary endodontic infections

Cited by 25 publications

References 37 publications

Antibacterial profiling of abietane-type diterpenoids

Antibacterial profiling of abietane-type diterpenoids

ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

In vitro evaluation of essential oils for potential antibacterial effects against Xylella fastidiosa

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