Antibacterial activity of florfenicol composite nanogels against <i>Staphylococcus aureus</i> small colony variants

Liu, Jinhuan; Ju, Mujie; Wu, Yifei; Leng, Nannan; Algharib, Samah Attia; Luo, Wanhe

doi:10.4142/jvs.22046

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…The formation of tildipirosin-loaded xanthan gum−gelatin composite nanogels were prepared based on previous studies. 19 Briefly, HP-β-CD (100, 200, or 300 mg) was completely dissolved in 5 mL of ultrapure water. 300 mg of tildipirosin was dissolved in 4 mL of ultrapure water and added into the HP-β-CD solution at 1500 rpm to prepare the tildipirosin−HP-β-CD−IC by inclusion.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

Li,

Liu,

Dou

et al. 2024

Langmuir

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Tildipirosin has no significant inhibitory effect on intracellular bacteria because of its poor membrane permeability. To this end, tildipirosin-loaded xanthan gum− gelatin composite nanogels were innovatively prepared to improve the cellular uptake efficiency. The formation of the nanogels via interactions between the positively charged gelatin and the negatively charged xanthan gum was confirmed by powder X-ray diffraction and Fourier transform infrared. The results indicate that the optimal tildipirosin composite nanogels possessed a 3D network structure and were shaped like a uniformly dispersed ellipse, and the particle size, PDI, and ζ potential were 229.4 ± 1.5 nm, 0.26 ± 0.04, and −33.2 ± 2.2 mV, respectively. Interestingly, the nanogels exhibited gelatinase-responsive characteristics, robust cellular uptake via clathrin-mediated endocytosis, and excellent sustained release. With those pharmaceutical properties provided by xanthan gum−gelatin composite nanogels, the anti-Staphylococcus aureus activity of tildipirosin was remarkably amplified. Further, tildipirosin composite nanogels demonstrated good biocompatibility and low in vivo and in vitro toxicities. Therefore, we concluded that tildipirosin-loaded xanthan gum−gelatin composite nanogels might be employed as a potentially effective gelatinase-responsive drug delivery for intracellular bacterial infection.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The formation of tildipirosin-loaded xanthan gum–gelatin composite nanogels were prepared based on previous studies . Briefly, HP-β-CD (100, 200, or 300 mg) was completely dissolved in 5 mL of ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

Li,

Liu,

Dou

et al. 2024

Langmuir

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“…Florfenicol (FLO) is a broad-spectrum chloramphenicol antibiotic widely used in the prevention and treatment of bacterial diseases, which can inhibit or eliminate the growth of microorganisms [ 1 , 2 , 3 , 4 , 5 ]. During aquaculture, FLO, as a pharmaceutical feed, is usually leached directly or released into the aquatic environment with feces [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Study on the Direct and Indirect Photolysis of Antibacterial Florfenicol in Water Using DFT/TDDFT Method and Comparison of Its Reactivity with Hydroxyl Radical under the Effect of Metal Ions

Kang,

Lu,

Wang

2024

Toxics

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Florfenicol (FLO) is a widely used antibacterial drug, which is often detected in the environment. In this paper, the photolysis mechanism of FLO in water was investigated using density functional theory (DFT) and time-dependent density functional theory (TDDFT). The focus of the study is to elucidate the direct photolysis mechanism of FLO in the water environment and the indirect photolysis of free radicals (·OH, ·NO3, and ·SO4−) as active species. The effect of metal ions Ca2+/Mg2+/Zn2+ on the indirect photolysis was also investigated. The results show that the direct photolysis of FLO involves C–C/C–N/C–S bond cleavage, the C5–S7 bond cleavage is most likely to occur, and the C17–C18 cleavage reaction is not easy to occur during the direct photodegradation of FLO. The indirect photolysis of FLO is more likely to occur in the environment than direct photolysis. The main indirect photolysis involves OH-addition, NO3-addition, and SO4-addition on benzene ring. The order of difficulty in the indirect photolysis with ·OH is C2 > C3 > C4 > C5 > C6 > C1, Ca2+ can promote the indirect photolysis with ·OH, and Mg2+/Zn2+ has a dual effect on the indirect photolysis with ·OH. In other words, Mg2+ and Zn2+ can inhibit or promote the indirect photolysis with ·OH. These studies provide important information for theoretical research on the environmental behavior and degradation mechanism of drug molecules.

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Old Antibiotics Can Learn New Ways: A Systematic Review of Florfenicol Use in Veterinary Medicine and Future Perspectives Using Nanotechnology

Trif

Cerbu

Olah

et al. 2023

Animals

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Florfenicol is a broad-spectrum bacteriostatic antibiotic used exclusively in veterinary medicine in order to treat the pathology of farm and aquatic animals. It is a synthetic fluorinated analog of thiamphenicol and chloramphenicol that functions by inhibiting ribosomal activity, which disrupts bacterial protein synthesis and has shown over time a strong activity against Gram-positive and negative bacterial groups. Florfenicol was also reported to have anti-inflammatory activity through a marked reduction in immune cell proliferation and cytokine production. The need for improvement came from (1) the inappropriate use (to an important extent) of this antimicrobial, which led to serious concerns about florfenicol-related resistance genes, and (2) the fact that this antibiotic has a low water solubility making it difficult to formulate an aqueous solution in organic solvents, and applicable for different routes of administration. This review aims to synthesize the various applications of florfenicol in veterinary medicine, explore the potential use of nanotechnology to improve its effectiveness and analyze the advantages and limitations of such approaches. The review is based on data from scientific articles and systematic reviews identified in several databases.

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Antibacterial activity of florfenicol composite nanogels against Staphylococcus aureus small colony variants

Cited by 3 publications

References 30 publications

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

Study on the Direct and Indirect Photolysis of Antibacterial Florfenicol in Water Using DFT/TDDFT Method and Comparison of Its Reactivity with Hydroxyl Radical under the Effect of Metal Ions

Old Antibiotics Can Learn New Ways: A Systematic Review of Florfenicol Use in Veterinary Medicine and Future Perspectives Using Nanotechnology

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