Antibacterial Activity of Extracts Prepared from Tropical and Subtropical Plants on Methicillin-Resistant Staphylococcus aureus.

Nitta, Tomoko; Arai, Takashi; Takamatsu, Hiromu; Inatomi, Yuka; Murata, Hiroko; Iinuma, Munekazu; Tanaka, Toŝhiyuki; Ito, Tetsuro; Asai, Fujio; Ibrahim, I. S.; Nakanishi, Teruyuki; Watabe, Kazuhito

doi:10.1248/jhs.48.273

Cited by 143 publications

(77 citation statements)

References 9 publications

(11 reference statements)

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“…Several Achillea plants have been found to possess antiseptic and infection preventing properties [38,39]. They are being used as ingredients in medicinal and cosmetic preparations [40].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Zeedan¹

2014

J Microb Biochem Technol

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Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in treatment of antibiotic-resistant bacteria. The present study aimed to evaluate the efficacy of essential oil extracted from Achillea fragrantissima plant growing in Egypt for antimicrobial, antiviral activities and chemical composition analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). We also performed determination of essential oil antimicrobial activity by agar desk diffusion method and Minimal Inhibitory Concentration (MIC). Also, antiviral activity on ORF virus by pock reduction test was performed. It was reduced virus titer from 5.9 to 1.00 at 180 minutes. Detection of beta lactams resistant bacteria (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) by PCR with primers of mecA gene and Bela gene. The essential oil obtained by hydrodistillation was analyzed by GC-MS. The major components were found to be Santolina triene (1.97%), 2,5,5-trimethyl-3,6-heptadien-2-ol (8.23%) Eucalyptol 8.17, trans-2,7-Dimethyl-4,6octadien-2-ol (24.40%), 1,5-Heptadien-4-one-3,6-trimethyl (7.65%), Artemisia alcohol (3.49%), à Thujone (33.97%), Cissabinol (1.92%), Lavandulol (0.71%), 2-Octen-4-ol, 2-methyl (2.02%), 3-Cyclohexen1ol,4-methyl1 (1 methylethyl) (CAS) (2.15%), à terpineol ( 0.05% ), Estragole (0.71%) Lavandulyl acetate (0.49%), Sabinyl acetate (2.12 %), Germacrened (0.94%). Finally, our study proved that the essential oil has bactericidal effect on some bacterial resistant antibiotic (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli ) as well as antiviral activity on ORF virus but it is still need further extensive studies for safety and drug interaction. Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential IntroductionMedicinal plants still constitute one of the major source of drugs in modern as well as traditional medicine throughout the world [1,2]. Since a long period of time, plants have been a valuable source of natural products for maintaining animals and human health [2,3]. The development of multidrug-resistant(MDR) bacteria takes place because of the accumulation of different antibiotic resistance mechanisms inside the same strain which able to live in the presence of antibiotics drugs so that standard treatments become ineffective [4,[14][15][16]. Although, the pharmacological companies have produced a number of new antibiotics recently, but even then microorganism resistance to antibiotics increased throughout the world [5,7]. This situation has attract attention of researchers towards herbal products for developing and improve the antibacterial drugs quality [6,7].Natural products of higher plants may possess a new source of antimicrobial agents with possibly novel mechanisms for treatment of infectious diseases [8,9]. Medicinal plants contain active principles which can be used as alternative effective herbal dru...

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“…Several Achillea plants have been found to possess antiseptic and infection preventing properties [38,39]. They are being used as ingredients in medicinal and cosmetic preparations [40].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Zeedan¹

2014

J Microb Biochem Technol

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“…In the present study, analyses of growth inhibition activity performed by using the well diffusion method showed that all the tested plants, which are commonly used by traditional medical practitioners, exhibited moderate activity against clinical isolates of bacterial strains at extract concentrations of 1 mg/ml Nitta et al, 2002;Voravuthikunchai and Kitpipit, 2005). Infections caused by multiple drug resistance, including resistance to β-lactam, are among the most difficult infections to treat (Abu-Shanab et al, 2004, 2006Kim et al, 2005).…”

Section: Discussionmentioning

confidence: 64%

“…The MIC and MBC values obtained for the extracts against the bacterial strains support the findings obtained by using the well diffusion method. One concern is that, for the extracts obtained in this study, MIC values were lower than MBC values, thus suggesting that these extracts are bacteriostatic at lower concentrations and bactericidal at higher concentrations (Abu-Shanab et al, 2006;Nitta et al, 2002;Ross et al, 2011).…”

Section: Discussionmentioning

confidence: 90%

Antibacterial activity of selected Dendrobium species against clinically isolated multiple drug resistant bacteria

Paudel¹,

Rajbanshi²,

Sah³

et al. 2018

Afr. J. Microbiol. Res.

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Dendrobium species are widely used in traditional medicine as remedies for tonic to nourish the stomach, promote the production of body fluids and decrease fever. In this study, the antibacterial activities of four traditionally used Dendrobium species were tested against clinically isolated multiple drug resistant (MDR) bacteria. Hexane, chloroform, acetone, ethanol and methanol extracts of Dendrobium amoenum, Dendrobium crepidatum, Dendrobium moniliforme and Dendrobium longicornu were tested for antibacterial activity against methicillin-resistant Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi and Acinetobacter baumannii by using the well diffusion method. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using the serial dilution method. For D. amoenum, hexane, chloroform and acetone extracts showed antibacterial activity against S. aureus (ZOI: 11.33, 12.00 and 11.00 mm respectively), acetone extract against A. baumannii (ZOI: 13.00 mm) and methanol extract against P. aeruginosa (ZOI: 12.00 mm). For D. crepidatum, hexane, chloroform, acetone, ethanol and methanol extracts showed antibacterial activity against S. typhi (ZOI: 10.00, 11.67, 12.00, 9.67 and 12.67 mm respectively), hexane, ethanol and methanol extracts against P. aeruginosa (ZOI: 10.00, 9.67 and 15.00 mm respectively), choloroform and acetone extracts against S. aureus (ZOI: 9.67 mm) and ethanol extract against E. coli (ZOI: 11.00 mm). For D. moniliforme, chloroform extract showed antibacterial activity against K. pneumoniae, P. aeruginosa, S. typhi, A. baumannii (ZOI: 12.67, 12.00, 11.67 and 13.00 mm respectively), acetone extract against S. aureus (ZOI: 11.00 mm) and A. baumannii (ZOI: 12.67 mm). For D. longicornu, chloroform extract showed antibacterial activity against A. baumannii (ZOI: 12.00 mm). MIC and MBC of these extracts of plants showed they have moderate antibacterial activity against the bacterial strains used. In conclusion, these Dendrobium species can be used as antibiotic agents.

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“…The reason may be due to the extracts' hydrophobicity, which caused cell rupture as a result of the destruction of the membrane's structure (Nitta et al, 2002).…”

Section: Ajptmentioning

confidence: 99%

Comparative Study of Antibacterial Activity of Plant Ex-Tracts From Several Regions of Asia

Al-Judaibi¹,

Al-Zahrani²,

Altammar³

et al. 2014

American Journal of Pharmacology and Toxicology

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This study was designed to investigate the antibacterial activities of alcohol extracts of Phoenix dactylifera (date palm 'type Ajwa'), Nigella sativa (black cumin), Elettaria cardamomum (cardamom), Tinospora crispa (Akar patawali) and Panax ginseng (ginseng). The plant extracts were prepared with methanol and assayed for antibacterial activity against Methicillin-Resistant Staphylococcus Aureus (MRSA) and S. aureus. Extracts of N. sativa, E. cardamomum and P. ginseng produced maximum inhibition activities in the MRSA strain ATCC 33591, while T. crispa had the greatest activity in the strain ATCC 25923. The P. dactylifera extract had no effect on the tested bacteria. The growth inhibition was used to determine minimum inhibitory concentrations and minimum bactericidal concentrations. An in vivo experiment using the T. crispa ethanol extract in adult male and female Sprague Dawley rats (4 g kg −1 dose) showed low toxicity based on the LD 50 value.

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Antibacterial Activity of Extracts Prepared from Tropical and Subtropical Plants on Methicillin-Resistant Staphylococcus aureus.

Cited by 143 publications

References 9 publications

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Antibacterial activity of selected Dendrobium species against clinically isolated multiple drug resistant bacteria

Comparative Study of Antibacterial Activity of Plant Ex-Tracts From Several Regions of Asia

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