Antibacterial Activity of Citrus Aurantifolia Leaves Extracts Against Some Enteric Bacteria of Public Health Importance

Zage, Abubakar U.; T, Sani Tajo; Ali, M. Syed

doi:10.32474/mams.2018.01.000107

Cited by 4 publications

(3 citation statements)

References 10 publications

(13 reference statements)

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“…The result of the antibacterial activity of C. aurantifolia leaves extracts in the present study was in conformity with studies that reported its antibacterial potentials (25,26). The molecular report of the target genes in the respective bacteria was confirmatory because they conform to the expected band size as reported by Woodford et al, (27), Zhang et al, (28) Maheux et al, (29) and Einas et al, (30).…”

Section: Discussionsupporting

confidence: 93%

Characterization of bacteria isolates colonizing the throat of hospitalized patients at Sobi Specialist Hospital, Ilorin, Nigeria and in vitro antimicrobial effects of Citrus aurantifolia and Alum on the isolates

Olajide¹,

Kolawole²,

Bada-Siyede³

et al. 2022

Af J Clin Exp Micro

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Background: Antibiotic resistance in microorganisms implicated in nosocomial respiratory infections is a major reason for prolonged hospital stay and increased cost of therapeutic treatment of hospital acquired pneumonia (HAP). This study was designed to isolate bacterial pathogens colonizing the throat of hospitalized patients at the Sobi Specialist Hospital, Ilorin, and to evaluate antibacterial effects of extracts of Citrus aurantifolia peel and Alum against these bacterial isolates. Methodology: This was a cross sectional study of 100 randomly recruited hospitalized patients at the Sobi Specialist Hospital, Ilorin, Nigeria. Throat samples collected from consenting participants were cultured on selective agar media (MacConkey, Eosin-Methylene blue and Mannitol salt) for isolation of bacteria. Identification of isolates from culture plates was done by Gram reaction and conventional biochemical tests while confirmation of the isolates was done by the polymerase chain reaction (PCR) assay. Antibiotic susceptibility test for each isolate to selected antibiotics (ampicillin, amoxicillin-clavulanate, cefuroxime, ceftazidime, gentamicin, nitrofuran, ofloxacin and ciprofloxacin) was done by the Kirby Buer disc diffusion method. Aqueous extract of Alum ([KAl(SO4).12H2O]) was done to produce concentrations of 10, 20, 30, 40 and 50% (w/v) at pH 3.6 and tested on the bacterial isolates using agar diffusion method. Citrus aurantifolia peel was extracted using methanol and hexane solvents to produce extract concentrations of 500mg/ml, 250mg/ml and 150mg/ml, and tested on the isolates by agar diffusion, and by the broth dilution method to obtain minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of C. aurantifolia. Results: A total of 14 bacterial isolates were recovered from throat samples of 100 hospitalized patients with Staphylococcus aureus (43%, n=6) being the most frequent while Escherichia coli (14.5%, n=2) was the least frequent. The isolates were generally resistant to penicillin, aminoglycoside and fluoroquinolone groups of antibiotics tested. The zone of inhibition for hexane and methanol extracts of C. aurantifolia and aqueous extract of alum on the bacterial isolates ranged from 11.5-19.2mm, 9.8-15.8mm, and 9.3-21.2mm respectively while those of selected antibiotics ranged from 7.0-25.0mm. The MICs of hexane and methanol extracts of C. aurantifolia against S. aureus were 10mg/ml and 25mg/ml, while the MBCs were 50 and 100mg/ml respectively. Conclusion: Findings from this study showed the presence of resistant pathogenic bacteria colonizing the throat of hospitalized patients receiving care at the Sobi Specialist Hospital, Ilorin, Nigeria. The crude extracts of C. aurantifolia and Alum in this study showed inhibitory effects (albeit at higher concentrations) on the bacterial isolates comparable to the standard antibiotics. We posit that based on the inhibition capacity, further studies to characterize, purify and isolate the active anti-bacterial components in the extracts should be considered for novelty.

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Section: Discussionsupporting

confidence: 93%

Characterization of bacteria isolates colonizing the throat of hospitalized patients at Sobi Specialist Hospital, Ilorin, Nigeria and in vitro antimicrobial effects of Citrus aurantifolia and Alum on the isolates

Olajide¹,

Kolawole²,

Bada-Siyede³

et al. 2022

Af J Clin Exp Micro

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“…The ethanolic extract of C. aurantifolia leaves exhibited higher antibacterial activity than the aqueous one against Klebsiella bacteria and Escherichia coli, though the isolates showed distinct susceptibility to one another [89]. It is possible that the higher activity of the ethanolic extract can be attributed to the better solubility of the secondary metabolites than the aqueous ones, thus leading to better efficacy.…”

Section: Antibacterialmentioning

confidence: 96%

Bioactive Components and Their Activities from Different Parts of Citrus aurantifolia (Christm.) Swingle for Food Development

Indriyani

Anshori

Permadi

et al. 2023

Foods

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Citrus aurantifolia is part of the Rutaceae family and belongs to the genus Citrus. It is widely used in food, the chemical industry, and pharmaceuticals because it has a unique flavor and odor. It is nutrient-rich and is beneficial as an antibacterial, anticancer, antioxidant, anti-inflammatory, and insecticide. Secondary metabolites present in C. aurantifolia are what give rise to biological action. Flavonoids, terpenoids, phenolics, limonoids, alkaloids, and essential oils are among the secondary metabolites/phytochemicals discovered in C. aurantifolia. Every portion of the plant’s C. aurantifolia has a different composition of secondary metabolites. Environmental conditions such as light and temperature affect the oxidative stability of the secondary metabolites from C. aurantifolia. The oxidative stability has been increased by using microencapsulation. The advantages of microencapsulation are control of the release, solubilization, and protection of the bioactive component. Therefore, the chemical makeup and biological functions of the various plant components of C. aurantifolia must be investigated. The aim of this review is to discuss the bioactive components of C. aurantifolia such as essential oils, flavonoids, terpenoids, phenolic, limonoids, and alkaloids obtained from different parts of the plants and their biological activities such as being antibacterial, antioxidant, anticancer, an insecticide, and anti-inflammatory. In addition, various extraction techniques of the compounds out of different parts of the plant matrix as well as the microencapsulation of the bioactive components in food are also provided.

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“…Ekstrak air daun jeruk nipis (Citrus aurantifolia) dapat menghambat pertumbuhan bakteri Salmonella typhi dan Escherchia coli dengan berbagai konsentrasi. Konsentrasi yang paling baik dalam menghambat pertumbuhan bakteri adalah 25% dengan nilai hambat 11,25 mm dan 12,33 mm (Zage et al, 2018).…”

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Formulasi Sediaan Gel Pembersih Gigi Infusa Daun Jeruk Nipis (Citrus aurantifolia Swingle.) Dan Uji Aktivitas Terhadap Bakteri Streptococcus mutans

Ridwan¹

2022

jpmw

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Gel pembersih gigi merupakan salah satu produk yang dapat membantu menjaga kesehatan gigi dan mulut dari bakteri penyebab kerusakan/ karies pada gigi. Bakteri penyebab karies gigi adalah bakteri Streptococcus mutans. Salah satu tanaman yang memiliki aktivitas sebagai antibakteri adalah daun jeruk nipis (Citrus aurantifolia Swingle.). Daun jeruk nipis mengandung senyawa tanin, flavonoid, dan alkaloid. Penelitian ini bertujuan untuk membuat formula sediaan gel pembersih gigi dan mengetahui aktivitasnya terhadap bakteri Streptococcus mutans.Penelitian ini merupakan jenis penelitian eksperimental laboratorium dengan menggunakan metode ekstraksi infusa yang dibuat gel pembersih gigi dengan tiga konsentrasi yaitu 15%, 20% dan 25% dan dilakukan uji stabilitas sediaan meliputi uji organoleptik, uji pH, uji viskositas, uji homogenitas, dan sineresis gel selama 4 minggu serta uji aktivitas antibakteri Streptococcus mutans dengan data hasil pengujian zona hambat dianalisis dengan menggunakan metode kruskall-wallis dan dilanjutkan dengan uji mann-whitney.Berdasarkan hasil penelitian yang diperoleh sediaan gel pembersih gigi berwarna coklat kekuningan, memiliki aroma khas pengaroma dan khas ekstrak samar, pH 6, sediaan homogen, uji viskositas masing-masing formula baik dan memenuhi syarat, dan pada pengujian sineresis gel didapatkan formula mengalami sineresis. Hasil uji bakteri daya hambat konsentrasi 15% 18,89 mm, konsentrasi 20% 21,40 mm, dan konsentrasi 25% 12,90 mm. Hasil analisis data dengan menggunakan uji kruskal-wallis menunjukan nilai p= 0,009 < 0,05 dan dapat disimpulkan sediaan gel pembersih gigi memiliki aktivitas antibakteri

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Antibacterial Activity of Citrus Aurantifolia Leaves Extracts Against Some Enteric Bacteria of Public Health Importance

Cited by 4 publications

References 10 publications

Characterization of bacteria isolates colonizing the throat of hospitalized patients at Sobi Specialist Hospital, Ilorin, Nigeria and in vitro antimicrobial effects of Citrus aurantifolia and Alum on the isolates

Characterization of bacteria isolates colonizing the throat of hospitalized patients at Sobi Specialist Hospital, Ilorin, Nigeria and in vitro antimicrobial effects of Citrus aurantifolia and Alum on the isolates

Bioactive Components and Their Activities from Different Parts of Citrus aurantifolia (Christm.) Swingle for Food Development

Formulasi Sediaan Gel Pembersih Gigi Infusa Daun Jeruk Nipis (Citrus aurantifolia Swingle.) Dan Uji Aktivitas Terhadap Bakteri Streptococcus mutans

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