Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain

Vasconcelos, Nathalie Gaebler; Silva, Késia Esther; Croda, Júlio; Simionatto, Simone

doi:10.1590/0037-8682-0032-2020

Cited by 10 publications

(7 citation statements)

References 14 publications

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“…The essential oils (EOs) produced by the distillation of aromatic plants are, among the natural substances, the products with the highest antimicrobial potential [ 12 , 13 ]. Several studies have been recently published to evaluate the antifungal activity of EOs against C. auris and its biofilm [ 14 , 15 , 16 , 17 ], and the Cinamomum cassia EO (CC-EO) is one of the most powerful with its bioactive compound cinnamaldehyde [ 18 , 19 ]. C. cassia Presl is a tropical aromatic evergreen tree of the Lauraceae family, commonly used in traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Rosato

Napoli

Granata

et al. 2023

Plants

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Background: Candida auris represents an emerging pathogen that results in nosocomial infections and is considered a serious global health problem. The aim of this work was to evaluate the in vitro antifungal efficacy of Cinnamomum cassia essential oil (CC-EO) pure or formulated in polycaprolactone (PCL) nanoparticles against ten clinical strains of C. auris. Methods: nanoparticles of PCL were produced using CC-EO (nano-CC-EO) and cinnamaldehyde (CIN) through the nanoprecipitation method. The chemical profile of both CC-EO and nano-CC-EO was evaluated using SPME sampling followed by GC-MS analysis. Micro-broth dilution tests were performed to evaluate both fungistatic and fungicidal effectiveness of CC-EO and CIN, pure and nano-formulated. Furthermore, checkerboard tests to evaluate the synergistic action of CC-EO or nano-CC-EO with micafungin or fluconazole were conducted. Finally, the biofilm disrupting activity of both formulations was evaluated. Results: GC-MS analysis shows a different composition between CC-EO and nano-CC-EO. Moreover, the microbiological analyses do not show any variation in antifungal effectiveness either towards the planktonic form (MICCC-EO = 0.01 ± 0.01 and MICnano-CC-EO = 0.02 ± 0.01) or the biofilm form. No synergistic activity with the antifungal drugs tested was found. Conclusions: both CC-EO and nano-CC-EO show the same antimicrobial effectiveness and are potential assets in the fight against C. auris.

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Section: Introductionmentioning

confidence: 99%

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Rosato

Napoli

Granata

et al. 2023

Plants

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“…Essential oils are important plant-derived compounds with potent biological activities that can be utilizable in agriculture, medicine, and biotechnology. Essential oils are also known to control the population kinetics of microbes, including bacteria [ 2 ], various viruses [ 3 ], infectious fungi [ 4 ] and so on. It is also evident that these essential oils inhibit the biofilm formation effects of various bacterial colonies [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Utilization of Pomelo (Citrus maxima) Peel Waste into Bioactive Essential Oils: Chemical Composition and Insecticidal Properties

Visakh

Pathrose

Narayanankutty³

et al. 2022

Insects

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The wastes generated during the post-harvest handling of various agricultural commodities is rather under-utlilized. The peels of citrus fruits are often discarded as waste. Citrus peels are rich in essential oils and exhibit toxicity towards various insect species. The essential oils are also an eco-friendly option for insect pest management. The Citrus maxima peel essential oil (CMEO), a waste product, characterized it, and evaluated its potential for insect pest management. The major terpenoids present in CMEO are Limonene and α-Pinene. The CMEO displayed potentials in controlling the insect pests via contact and fumigant toxicity. Moreover, CMEO showed significant larvicidal activities against Culex tritaeniorhynchus and Aedes aegypti species of mosquitoes; however, Armigeres subalbatus was more resistant. The biological safety of the essential oil was also tested against the stored seeds, where no significant inhibition of seed germination was noticed compared to the control. Utilizing a waste product such as citrus peel for pest management can achieve the dual objective of waste utilization and eco-friendly pest management. Overall, the CMEO is therefore found to be a bioactive essential oil extracted from the wastes of pomelo (C. maxima).

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“…Each experiment was repeated three times. One unit of AKP activity(expressed as U/g Prot) was defined as 1 mg of phenol produced by 100 ml of culture solution interacting with the matrix in a 15-minute interval [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

In-vitro antibacterial activity and mechanism of Monarda didyma essential oils against Carbapenem-resistant Klebsiella pneumoniae

Chen,

Zhao,

Liu

et al. 2023

BMC Microbiol

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To fight the global epidemic of drug-resistant bacteria, essential oils have gained increasing attention as a new source of antibiotics. The antimicrobial activity of Monarda didyma essential oils (MDEO) for the Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains were determined by agar disc diffusion assay and broth microdilution assay. To further understand MDEO efficacy, a time-growth curve was performed. The biofilm formation of CRKP were determined by crystalline violet staining method, additionally, changes in intracellular Adenosine triphosphate (ATP), protein, Alkaline phosphatase (AKP) activities, and membrane integrity were investigated to assess the influence of MDEO on cell membrane damage. Finally, the activities of key enzymes in the tricarboxylic acid (TCA) pathways and pentose phosphate (PPP) pathways were examined to determine the effect of MDEO on the respiratory metabolism of CRKP. This study presents the antibacterial mechanism of MDEO against CRKP with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 1.25 mg/ml. To understand MDEO efficacy, a time-kill kinetics approach was performed. The bactericidal effect of MDEO was evident at 2 h compared to the control at its MIC and 2MIC. Surface electron microscopic and ATP assay studies provided evidence for the multi-target action of MDEO against CRKP. MDEO could inhibit CRKP biofilm formation. MDEO could also cause irreversible damage to the CRKP cell membrane, resulting in the leakage of biological macromolecules (protein, ATP) and the reduction of intracellular enzymes (AKP) activities. Finally, MDEO affected the pathways of respiratory metabolism, such as PPP and TCA pathways. MDEO could reduce the activity of key enzymes (Glucose-6-phosphate dehydrogenase, citrate synthase, isocitrate dehydrogenase, and α-ketoglutarate dehydrogenase) in the PPP and TCA pathways to exert its biological effects against CRKP. These results suggest MDEO can exert inhibitory effects on CRKP, and potential mechanisms of action including inhibition of biofilm formation, damage of cell membrane structure and inhibition of energy metabolism.

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Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain

Cited by 10 publications

References 14 publications

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Utilization of Pomelo (Citrus maxima) Peel Waste into Bioactive Essential Oils: Chemical Composition and Insecticidal Properties

In-vitro antibacterial activity and mechanism of Monarda didyma essential oils against Carbapenem-resistant Klebsiella pneumoniae

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