Antibacterial activity of agricultural waste derived wollastonite doped with copper for bone tissue engineering

Azeena, S; Subhapradha, Namasivayam; Selvamurugan, N.; Narayan, Shoba; Srinivasan, Narasimhan; Murugesan, Ramachandran; Chung, TW; Moorthi, A.

doi:10.1016/j.msec.2016.11.118

Cited by 49 publications

(36 citation statements)

References 39 publications

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“…The seeded cells were treated with various concentrations (0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 and 2 mg/mL) of nBGC & Zn-nBGC along with triton-X-100 as positive control and incubated for 24 h. After 24 h, the medium was removed and 500 μl of 0.5 % MTT solution was added into the each well followed by 4 h incubation. 100 μl of DMSO was added into each well and incubated for another2 h. The viable cells were determined by calorimetric analysis at 570 nm [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Sol–gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration

Paramita

Murugesan

Narashiman

et al. 2021

J Mater Sci: Mater Med

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Bone is a flexible and electro active tissue that is vulnerable to various traumatic injuries. The self-healing of damaged bone tissue towards reconstruction is limited due to the lack of proper niche compliances. Nevertheless, the classical grafting techniques like autograft/allograft for bone repair pose challenges like bacterial infections and donor-site morbidity with unsatisfactory outcomes. The use of appropriate biomaterial with osteogenic potential can meet these challenges. In this regard, bioactive glass ceramics is widely used as a bone filler or graft material because of its bonding affinity to bone leading towards bone reconstruction applications without the challenge of post implant infections. Hence, the current study is aimed at addressing this potentiality of zinc (Zn) for doped the bioglass at nano-scale advantages for bone tissue repair. Since, Zn has been demonstrated to have not only antibacterial property but also the stimulatory effect on osteoblasts differentiation, mineralization by enhancing the osteogenic genes expression. In view of these, the present study is focused on sol–gel synthesis and pysico-chemical characterization of Zinc-doped bioglass nanoparticles (Zn-nBGC) and also analyzing its biological implications. The surface morphological and physiochemical characterizations using SEM, EDX, FT-IR and XRD analysis has shown the increased surface area of Zn-nBGC particles providing a great platform for biomolecular interaction, cytocompatibility, cell proliferation and osteogenic differentiation. The obtaining hydroxy apatite groups have initiated in vitro mineralization towards osteogenic lineage formation. Zn has not only involved in enhancing cellular actions but also strengthen the ceramic nanoparticles towards antibacterial application. Hence the finding suggests a biomaterial synthesis of better biomaterial for bone tissue engineering application by preventing post-operative bacterial infection.

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Section: Methodsmentioning

confidence: 99%

Sol–gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration

Paramita

Murugesan

Narashiman

et al. 2021

J Mater Sci: Mater Med

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“…Ismail et al produced wollastonite using rice husk as a source of silica and commercial calcium oxide by autoclaving and sintering method. Azeena et al reported a synthesis of wollastonite from rice straw ash as silica source and calcium nitrate by sol‐gel method. Wang et al produced wollastonite by silicon slag from zirconium oxychloride production industries with the addition of certain amount of calcium oxide.…”

Section: Introductionmentioning

confidence: 99%

A comparative study on in vitro behavior of calcium silicate ceramics synthesized from biowaste resources

et al. 2019

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Calcium silicate ceramics have received significant attention for biomedical applications for their excellent bioactivity and osteoconduction properties. Sol‐gel process is extensively used for the fabrication of calcium silicates. In sol‐gel process, calcium nitrate tetra hydrate (Ca(NO3)2·4H2O) and tetraethylorthosilicate (TEOS) are used as precursors. However, these precursors are expensive. The objective of this work was to compare in vitro behavior of calcium silicate (CaSiO3) produced using biowaste such as rice husk ash (RHA) and eggshells (coded; NCS) with CaSiO3 prepared using TEOS and Ca(NO3)2·4H2O (coded; CCS). Thermal investigation results revealed that the crystallization temperature for NCS is relatively lower (772°C) than for CCS (870°C). Bioactivity was studied in vitro using simulated body fluid (SBF) with respect to mineralization rate of hydroxyapatite. Mineralization of a greater hydroxyapatite was observed on NCS ceramics than CCS ceramics after incubation for 3, 7, 14 days in SBF solution, which was confirmed using X‐ray diffractometer, Fourier transform infrared spectroscopy, scanning electron microscopy‐energy dispersive spectroscopy. Degradation studies were conducted in Tris‐HCl solution and the test results revealed that NCS ceramics has lower dissolution rate than CCS ceramics. The antimicrobial assay has shown that NCS samples exhibit significant zone of inhibition against Escherichia coli and Staphylococcus aureus which confirmed that the CaSiO3 prepared from RHA and eggshell can prevent bacteria from adhering to the surface. In addition cell culture studies revealed that NCS ceramics possess good cytocompatibility with MG‐63 cells and significantly promoted cell proliferation.

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“…Ws and Cu‐Ws particles were synthesized using agricultural waste product by sol‐ gel method in our previous publication and their physiological characteristics were analyzed using various techniques 17 . The fabrication of the scaffolds was executed as reported earlier 18 .…”

Section: Methodsmentioning

confidence: 99%

Polycaprolactone fibrous electrospun scaffolds reinforced with copper doped wollastonite for bone tissue engineering applications

et al. 2020

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The bone defects healing are always associated with post implantation infections; hence biomaterials rules significant role for orchestration of defective bone. In this study, we synthesized biocomposite scaffold by combining polycaprolactone (PCL), wollastonite (Ws) and metal ions (Cu) by electrospinning technique. The manufactured scaffolds (PCL/Ws andPCL/Cu–Ws) were subjected to physio‐chemical characterization by scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier Transform Infra Red Spectroscopy (FTIR) and XRD. The surface topography of the scaffolds was found to be micro‐fibrous in nature and each fiber was cylindrical in structure. The exogenous biomineralization and protein adsorption capacity of these scaffolds were studied. Enhanced amount of protein was adsorbed on PCL/Cu‐Ws than PCL/Ws scaffold after incubating for 48 hr in foetal bovine serum (FBS) also the biomineralization shown to be promoted the apatite formation in vitro. The synthesized PCL/Cu–Ws scaffold was biocompatible to mouse mesenchymal stem cells and enhanced the mRNA expressionof osteoblastic specific marker genes including alkaline phosphatase and type I collagen and major transcription factor Runx2 in the presence of osteogenic medium indicates the osteoconductive nature of the scaffolds. The amount of calcium deposition and promotion of osteoblast differentiation and mineralization on human osteoblast cells was confirmed by alizarin red staining. The fabricated scaffolds possess potent antibacterial effect against Staphylococcu aureus and Escherichia coli. Hence, our outcomes confirmed that the PCL/Ws and PCL/Cu–Ws scaffolds promote bonesynthesis by cell proliferation and differentiation suitable for applications in bone regeneration orbone defects.

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Antibacterial activity of agricultural waste derived wollastonite doped with copper for bone tissue engineering

Cited by 49 publications

References 39 publications

Sol–gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration

Sol–gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration

A comparative study on in vitro behavior of calcium silicate ceramics synthesized from biowaste resources

Polycaprolactone fibrous electrospun scaffolds reinforced with copper doped wollastonite for bone tissue engineering applications

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