Antibacterial Activity and Toxicological Evaluation of Semi Purified Hexane Extract of Urtica dioica Leaves

Singh, Rajdeo; Dar, Shoaib Ali; Sharma, Poonam

doi:10.3923/rjmp.2012.123.135

Cited by 37 publications

(10 citation statements)

References 23 publications

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“…Motamedi et al [38] found that ethanolic and methanolic extracts of stinging nettle leaves showed American Journal of Plant Sciences antibacterial activity against S. aureus, however the extraction solvent appeared to play an important role toward the outcome for antibacterial susceptibility tests. When examining the antibacterial activity of stinging nettle leaves by the disc sensitivity method, it was found that plant extracts obtained when using the solvent hexane were comparable to standard antibiotic Gentamycin, while chloroform, methanol, ethyl acetate, and aqueous extracts showed moderate to no activity against a variety of bacteria [36].…”

Section: Discussionmentioning

confidence: 99%

“…However, the composition of stinging nettle may differ, potentially caused by differences in planting, climatic, seasonal, and experimental conditions [43]. The extracting solvent may also influence the antibacterial activity of its bioactive components [36]. Other compounds such as terpenes, fatty acid esters and phenols were reported to be the antibacterial compounds in stinging nettle leaves against bacteria [36] [43].…”

Section: Discussionmentioning

confidence: 99%

“…Results for zones of inhibition showing equal to or greater than 7 mm (6 mm being the diameter of the filter discs) were considered active against the bacterial populations tested [36]. Nootka rose extracts showed showed significantly greater zones of inhibition (> 12 mm) for all three bacterial species tested when compared to results for stinging nettle extracts, where all zones of inhibition measured at < 12 mm (Table 1).…”

Section: Kirby Bauer Disc Sensitivity Testingmentioning

confidence: 99%

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Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of Rosa nutkana C. Presl (Nootka Rose) and Urtica dioica L. (Stinging Nettle) Extracts

Gendron¹,

Nilson²,

Ziffle³

et al. 2021

AJPS

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Nootka rose (Rosa nutkana C. Presl) and stinging nettle (Urtica dioica L.) have been traditionally used in the treatment of skin infection by Indigenous peoples of Vancouver Island, British Columbia, Canada. The main objective of this study was to examine the antibacterial efficacy of extracts of Nootka rose and stinging nettle against the common pathogenic skin bacteria Staphylococcus aureus, Micrococcus luteus, and Pseudomonas aeruginosa using Indigenous science and standard methods of analysis. The Indigenous science method of plant extraction by steeping as advised by the Traditional Knowledge keeper was performed to examine minimum inhibitory concentration (MIC) values and minimum bactericidal concentrations (MBC) by serial dilution and bacterial population counts. Soxhlet extractions and Kirby Bauer disc sensitivity testing showed that Nootka rose extracts possessed antibacterial effectiveness against all three bacterial species while stinging nettle extracts were effective against M. luteus. Results for MIC and MBC indicated antibacterial activity against M. luteus and S. aureus for the Nootka rose when using full-strength solutions; all three bacterial species exhibited growth when undiluted stinging nettle treatments were used. When considering bacterial population counts for S. aureus, results indicated that only the Nootka rose treatment offered effective inhibition. Chemical analysis showed that alkaloid percentage was greater in the stinging nettle (0.17%) than Nootka rose (0.07%), while saponin percentage was greater in the Nootka rose (0.87%) than stinging nettle (0.17%). Overall, Nootka rose showed a greater level of antibacterial effectiveness than stinging nettle by Indigenous

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Kirby Bauer Disc Sensitivity Testingmentioning

confidence: 99%

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Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of Rosa nutkana C. Presl (Nootka Rose) and Urtica dioica L. (Stinging Nettle) Extracts

Gendron¹,

Nilson²,

Ziffle³

et al. 2021

AJPS

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“…Multiple studies have also reported that the medicinal properties of UD include bleeding control and wound and burn healing. It also acts as a diuretic [6][7][8]. Moreover, crude UD extract has been reported as an antimicrobial agent against Gram-positive and Gram-negative microorganisms [9].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Synthesis of Silver Nanoparticles Using Ethyl Acetate Extract of Urtica diocia Leaves; Characterizations and Emerging Antimicrobial Activity

et al. 2022

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The current work reports the biosynthesis of silver nanoparticles (AgNPs) using the antimicrobial activities of ethyl acetate extract of Urtica diocia (UD) leaves as a reducing and capping agent. The synthesized UD-AgNPs were characterized using UV–visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray analysis (EDAX), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and dynamic light scattering (DLS). The UD-AgNPs were evaluated against Gram-positive and Gram-negative bacteria, and their size, shape, and distribution were recorded. The average size of an NP was 19.401 nm. The zone of inhibition (ZOI) for 75 µL of UD-AgNPs against Pseudomonas aeruginosa (P. aeruginosa) was 21 ± 0.4 mm more than that of the control drug Ciprofloxacin (16 ± 10 mm). The minimum inhibitory concentration (MIC) was the lowest against Escherichia coli (E. coli) (36 ± 3 µg/mL) and Staphylococcusepidermidis (S. epidermidis) (38 ± 3 µg/mL). Moreover, the minimum bactericidal concentration (MBC) was the lowest against E.coli (75 ± 00 µg/mL) and Enterococcus faecalis (E. faecalis (83 ± 16 µg/mL). Thus, the UD-AgNPs synthesized using the ethyl acetate extract of UD can be used as a new antimicrobial drug.

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“…/ Biosaintifika 14 (3) (2022): 356-363 Ramie (Boehmeria nivea) is a plant that belongs to the Urticaceae family. B. nivea contains active compounds, including alkaloids, lignans, flavonoids, terpenoids, glycosides, carotenoids, and terpenes (Singh et al, 2012). It has been reported that ramie has bioactivity such as antibacterial (Jung et al, 2020), antioxidant (Chen et al, 2014), and anti-inflammatory (Sung et al, 2013) effects.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Bioactive Compounds N-Hexane Extract of Ramie Leaves and Flowers (Boehmeria nivea) and Antibacterial Test Against Escherichia coli and Klebsiella pneumoniae

2022

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Ramie (Boehmeria nivea) is one of the Urticaceae family plants with various potential for pharmacological development. In this study, we analyzed the bioactive content of the n-hexane extract of B. nivea leaves and flowers, and the crude extract was tested for antibacterial activity. The metabolites in the leaves and flowers of ramie were analyzed using a Gas Chromatography-Mass Spectrometer (GC-MS). Antibacterial potential analysis was carried out by disc diffusion method using n-hexane extract of ramie leaves and flowers at concentrations of 20%, 40%, 60%, 80%, and 100% in inhibiting Escherichia coli and Klebsiella pneumoniae. Antibacterial potential was observed based on the average result of three observations of the diameter of the clear zone to determine the effectiveness of the extract. The results showed that the metabolome data of the n-hexane extract of ramie leaves were obtained as many as 40 compounds. Meanwhile, ramie flower metabolome analysis obtained 47 compounds, but one compound has not been identified. The crude n-hexane extract of B. nivea flower gave antibacterial activity at 80% and 100% concentrations against E. coli with an average clear zone of 9.15 ± 0.31 mm and 10.29 ± 0.28 mm. In contrast, the n-hexane extract of B .nivea leaves did not show any antibacterial activity. The results of confirmation of the dominant compound by GC-MS showed that the n-hexane extract consisted of tetratetracontane, tetracontane, Stigmast-5-en-3-ol,(3.beta.,24S)-(CAS)Clionasterol, methyl commate D, and 2,6,10,14,18,22-Tetracosahexsaene,2,6,10,15,19,23-hexamethyl-, (all-E). The n-hexane extract of B. nivea is recommended for other potential biological activities, such as anti-inflammatory, antioxidant, and analgesic.

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Antibacterial Activity and Toxicological Evaluation of Semi Purified Hexane Extract of Urtica dioica Leaves

Cited by 37 publications

References 23 publications

Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of <i>Rosa nutkana</i> C. Presl (Nootka Rose) and <i>Urtica dioica</i> L. (Stinging Nettle) Extracts

Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of <i>Rosa nutkana</i> C. Presl (Nootka Rose) and <i>Urtica dioica</i> L. (Stinging Nettle) Extracts

Biomimetic Synthesis of Silver Nanoparticles Using Ethyl Acetate Extract of Urtica diocia Leaves; Characterizations and Emerging Antimicrobial Activity

Analysis of the Bioactive Compounds N-Hexane Extract of Ramie Leaves and Flowers (Boehmeria nivea) and Antibacterial Test Against Escherichia coli and Klebsiella pneumoniae

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Antibacterial Activity and Toxicological Evaluation of Semi Purified Hexane Extract of Urtica dioica Leaves

Cited by 37 publications

References 23 publications

Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of &lt;i&gt;Rosa nutkana&lt;/i&gt; C. Presl (Nootka Rose) and &lt;i&gt;Urtica dioica&lt;/i&gt; L. (Stinging Nettle) Extracts

Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of &lt;i&gt;Rosa nutkana&lt;/i&gt; C. Presl (Nootka Rose) and &lt;i&gt;Urtica dioica&lt;/i&gt; L. (Stinging Nettle) Extracts

Biomimetic Synthesis of Silver Nanoparticles Using Ethyl Acetate Extract of Urtica diocia Leaves; Characterizations and Emerging Antimicrobial Activity

Analysis of the Bioactive Compounds N-Hexane Extract of Ramie Leaves and Flowers (Boehmeria nivea) and Antibacterial Test Against Escherichia coli and Klebsiella pneumoniae

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Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of <i>Rosa nutkana</i> C. Presl (Nootka Rose) and <i>Urtica dioica</i> L. (Stinging Nettle) Extracts

Antimicrobial Effectiveness on Selected Bacterial Species and Alkaloid and Saponin Content of <i>Rosa nutkana</i> C. Presl (Nootka Rose) and <i>Urtica dioica</i> L. (Stinging Nettle) Extracts