Antibacterial Activity and Reversal of Multidrug Resistance of Tumor Cells by Essential Oils from Fresh Leaves, Flowers, and Stems of Montanoa quadrangularis Schultz Bipontinus (Asteraceae) Collected in Mérida—Venezuela

Abstract

Essential oils obtained by hydrodistillation of Montanoa quadrangularis leaves, flowers, and stems were analyzed by GC and GC/MS techniques revealing myrcene, limonene, β-phellandrene, and sabinene among the main components. The aim of the present study was to evaluate the MDR modulator activity on human MDR1 gene transfected mouse lymphoma cell line and the antimicrobial activity on the essential oils obtained from different parts of the species under investigation. The results revealed that MQL caused a similar increase in the fluorescence activity of the cells at 0.02 μL/mL comparing to the Verapamil® value. The antimicrobial assay was carried out according to the disc diffusion method. Five different bacterial strains (Staphylococcus epidermidis, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli AG 100, and Escherichia coli AG100A) were treated with the essential oils and the zones of inhibition were determined on TSA plates and TSA agar plates supplemented with Tween 20. MQF and MQL showed activity against B. subtilis, S. epidermidis, and E. coli AG 100A while MQS was only active against E. coli AG 100A on TSA agar plates experiment. In case of TSA agar plates supplemented with 0.1 v/v% Tween 20 detergent, MQF showed inhibition on B. subtilis, S. epidermidis, and E. coli AG 100A; MQL was active against B. subtilis, E. coli AG 100, and E. coli AG 100A while MQS was only active against E. coli AG 100A.

Chemical Composition and Antimicrobial Potential of Essential Oil of Acritopappus confertus (Gardner) R.M.King & H.Rob. (Asteraceae)

“…[ 31 , 32 ], Pteronia onobromoides DC. [ 33 ], Glebionis coronaria (L.) Tzvelev [ 34 ], Vernonia polyanthes Less [ 35 ], Coreopsis triloba SF Blake [ 36 ], and Montanoa quadrangularis Schultz Bipontinus [ 37 ].…”

Phytochemical, Antioxidant, Antihyaluronidase, Antityrosinase, and Antimicrobial Properties of Nicotiana tabacum L. Leaf Extracts

“…For disc preparation, 10 μ l of each extract was dropped onto a 5 mm diameter paper disc and then plated on a TSA plate containing bacteria, and incubated at 37°C for 24 h. Reference antibiotics used in this study were 25 μ g of Ampicillin® and Penicillin®. The inhibitory zones around the discs were observed, measured, and expressed in mm [ 30 ]. All the experiments were carried out in triplicate.…”

Antimicrobial Properties Related to Anti-Acne and Deodorant Efficacy of Hedychium coronarium J. Koenig Extracts from Pulsed Electric Field Extraction

“…The anti-acne activities of H. coronarium extracts were investigated through their ability to inhibit C. acnes ATCC 6919 using the agar-well diffusion method [46]. Furthermore, the minimum inhibitory concentrations (MICs) were evaluated via the broth dilution method [47].…”

“…The deodorant effects of H. coronarium extracts were investigated through their ability to inhibit the microbial related to body odor, including S. aureus ATCC 25923, B. subtilis TISTR 008, M. luteus TISTR 884, P. aeruginosa ATCC 27853, and E. coli ATCC 25922, using the agar-well diffusion method [46]. Furthermore, the minimum inhibitory concentrations (MICs) were evaluated via the broth dilution method [47].…”