Antibacterial Activity and Mode of Action of &lt;i&gt;Mentha arvensis&lt;/i&gt; Ethanol Extract against Multidrug-Resistant &lt;i&gt;Acinetobacter baumannii&lt;/i&gt;

Zhang, Ling; Xu, Shu-gen; Wei, Liang; Mei, Jun; Di, Yue-ying; Lan, Huihua; Yang, Yan; Wang, Weiwei; Luo, Yuanyuan; Hou-zhao, Wang

doi:10.4314/tjpr.v14i11.21

Cited by 36 publications

(28 citation statements)

References 12 publications

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“…This confirms that EOs severely interfere with the lipid layer of cell membranes and cause intracellular leakages, followed by cell lysis [32]. Zhang et al [33] analyzed the protein leakage effect of the ethanol extract of Mentha arvensis on multidrug-resistant Acinetobacter baumannii . The intracellular protein released by the application of EO from A. sativum was significantly higher than that of intracellular protein released from Acinetobacter baumannii , which induced dose- and time-dependent rupture of the bacterial cell [34].…”

Section: Resultsmentioning

confidence: 72%

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

et al. 2019

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This study explored the chemical compositions of garlic essential oil, the inhibitory activity of garlic essential oil and diallyl disulfide (DADS) against Phytophthora nicotianae, and the effects on mycelial plasma membrane permeability and P. nicotianae inhibition. In total, 29 compounds were detected in garlic essential oil, of which 26 were detected by gas chromatography‒mass spectrometry (GC-MS) and 21 by headspace solid-phase microextraction (HS-SPME) GC-MS. DADS (60.12% and 19.09%) and trisulfide di-2-propenyl (14.18% and 17.98%) were the major components identified by HS-SPME GC-MS and GC-MS analysis, respectively. Half-inhibitory concentration (Ec50, antagonism) and minimum inhibitory concentration (MIC, fumigation) of DADS against P. nicotianae were 150.83 μL/L and 20 μL/L, respectively, while Ec50 of garlic essential oil was 1108.25 μL/L. Mycelial membrane permeability gradually increased in a concentration-dependent manner, and cell death increased at 450 μL/L DADS. Furthermore, DADS treatment significantly reduced the incidence of tobacco black shank and the number of P. nicotianae pathogens in rhizosphere soil. DADS also promoted root development of tobacco seedlings at low concentrations, which was inhibited at high concentrations. Therefore, DADS may play an important role in the antifungal effect against P. nicotianae by destroying mycelial cell membrane integrity, causing an increase in cell membrane permeability, and leading to cell death.

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Section: Resultsmentioning

confidence: 72%

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

et al. 2019

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“…This assay relies on the principle that, after diffusion into the cell, DCFDA gets deacetylated by cellular esterases to a nonfluorescent compound, which is later oxidized by ROS into 2',7'-dichlorofluorescein (DCF). DCF, being a highly fluorescent compound, can be detected by fluorescence spectroscopy with maximum excitation and emission spectra of 495 nm and 528 nm, respectively [17].…”

Section: Resultsmentioning

confidence: 99%

“…Herbal extract-induced generation of free radicals, e.g., ROS, peroxide radicals, and superoxide radicals, within bacterial cells, leading to cellular membrane damage, has been reported to be a crucial antibacterial mechanism of action [17]. Keeping this in view, levels of ROS generated within the bacterial cells were estimated using DCFDA.…”

Section: Resultsmentioning

confidence: 99%

“…The protein leakage induced within the bacterial pathogen's cellular environment upon exposure to specific GLF and GLM aqueous extracts and PRFs screened for potent antibacterial activity was evaluated by Bradford's method using a protocol reported elsewhere [16, 17]. According to this method, 16 to 18 h cultures of the bacteria cultured in nutrient broth were washed with 0.8% physiological saline by centrifugation at 10,000 rpm for 20 min, followed by resuspension in saline.…”

Section: Methodsmentioning

confidence: 99%

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Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Mishra

Joshi

Rajput

et al. 2018

Advances in Pharmacological Sciences

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Ganoderma lucidum (G. lucidum) fungus (Family Ganodermataceae) is widely used as a traditional medicine in China, Japan, and many Asian countries on account of its numerous medicinal properties such as antioxidant, anticancer, antimicrobial, energy enhancing, and immunostimulatory. This broad spectrum of therapeutic effects exhibited by G. lucidum is ascribed to its abundance in several classes of chemical constituents, namely, carbohydrates, flavonoids, minerals, phenolics, proteins, and steroids which possess substantial bioactivities. The aim of the current study was to prepare phenolic rich fractions (PRFs) from aqueous extract of the Indian variety of G. lucidum mycelium and fruiting body. These fractions were assessed for their antioxidant capacity by TPC (total phenolic content), TFC (total flavonoid content), FRAP (ferric reducing antioxidant power), and ABTS [2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid] assays. Quantification of flavonoids and nucleobases present in the fractions was carried out by high-performance thin layer chromatography (HPTLC). The antibacterial activity of the fractions was evaluated against Escherichia coli, Salmonella typhi, and Staphylococcus aureus. The antibacterial mechanism of action of the PRFs was established to be generation of reactive oxygen species and leakage of proteins within bacterial cells. Additionally, the protective effect of the PRFs in counteracting hypoxia was observed in HEK 293 cell lines.

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“…ex Schult also have shown their antimicrobial action by blebbing and leakage of cellular contents (Saritha et al 2015 ). The ethanolic extract of Mentha arvensis also induce cellular damage which increased with an increase in concentration (Zhang et al 2015 ). Scanning electron microscopic analysis of S. mutans and A. actinomycetemcomitans has revealed that cells after treatment of aqueous extract of A. lakoocha lost their original shape having an irregular, distorted cell wall structure (Teanpaisan et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

et al. 2017

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Phytochemicals of Syzygium cumini are used for the treatment of various diseases as a traditional medicine but the mechanism behind their action is not well reported. Antimicrobial activity of methanolic seed extract of S. cumini was done by agar well diffusion assay on Bacillus subtilis and its zone of inhibition was found to be 20.06 mm in comparison to control having no zone of inhibition. MIC of S. cumini was found to be 0.3 mg/ml. Genomic DNA degradation of B. subtilis reveals apoptosis and FE-scanning electron microscope indicates cell wall cracking on several intervals of time. Results of propidium iodide staining showed few bacterial cells were stained in control; however population of stained cells increased after exposing them for varying period of time. Flow cytometric kinetic data analysis on the membrane permeabilization in bacterial cell showed the significant contribution of antimicrobial potential of the seed extract on antimicrobial-induced permeabilization. In silico analysis revealed two components of S. cumini methanolic extract to be active against four enzymes (PDB ID—1W5D, 4OX3, 3MFD and 5E2F) which are crucial for plasma membrane synthesis in B. subtilis. Moreover lupeol showed highest binding energy for macromolecule 1W5D and 4OX3 forming one hydrogen bond each whereas stigmasterol showed the highest binding energy for macromolecule 3MFD and 5E2F forming four hydrogen bonds and alkyl bonds respectively. It demonstrates that methanolic seed extracts of S. cumini could be used for inhibition of food born infection caused by B. subtilis and also an alternative of prevalent antibiotics.

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Antibacterial Activity and Mode of Action of <i>Mentha arvensis</i> Ethanol Extract against Multidrug-Resistant <i>Acinetobacter baumannii</i>

Abstract: Purpose: To evaluate the antibacterial effect of ethanol extract of Mentha arvensis against multi-drug resistant Acinetobacter baumannii using liquid chromatography-mass spectrometry (LC-ESI-MS

Cited by 36 publications

References 12 publications

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Antimicrobial action of methanolic seed extracts of Syzygium cumini Linn. on Bacillus subtilis

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