Antibacterial activity and mechanism of phillyrin against selected four foodborne pathogens

Junshun, Zhang; Gao, Mingkun; Chai, Yangyang; Guo, Yang; Bao, Yihong; Tianzhi, Yang

doi:10.1590/fst.32922

Cited by 3 publications

(1 citation statement)

References 36 publications

(36 reference statements)

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“…The activity of Na + /K + -ATPase was measured as described previously (Zhang et al, 2022). The spore suspension was treated with ultrasonic cell breaker in an ice bath for 3 s (the gap was 10 s) and repeated for 30 times.…”

Section: Analysis Of Atpase Activitymentioning

confidence: 99%

Effect of pressure-assisted thermal sterilization combining with ε-polylysine on Bacillus subtilis spore proteins, nucleic acids and other intraspore substances

et al. 2023

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Pressure-assisted thermal sterilization (PATS) is a new technology to inactivate bacterial spores and ensure food safety. Little has been known about the effects of PATS combining with ε-PL on the spore's nucleic acid, enzymes and other key substances. This study aimed to investigate the inactivation effect of PATS combining with ε-PL on the spores of B. subtilis. The spores were treated with pressure 600 MPa at 25 °C, 65 °C and 75 °C, and ε-PL at 0.1% and 0.3%. After treatment, the survival rate of B. subtilis spores, leakage of nucleic acid and protein, the change in the cell membrane ATPase activity, the leakage of dipicolinic acid, and the damage on protein and nucleic acid of the spores were determined. The results showed that PATS combining with ε-PL inactivated more spores, and significantly increased the release of protein and nucleic acid compared to the control. ATPase activity reached the lowest value after the treatment of 600 MPa/75 °C combining with 0.3% ε-PL. The release of dipicolinic acid from the spores was increased by 600 MPa/75 °C combining with 0.3% ε-PL as compared with 600 MPa/75 °C treatment alone. FTIR analysis showed that a combination of PATS with ε-PL changed the spectral features of B. subtilis functional groups of proteins and nucleic acids. The PATS treatments when combined with ε-PL were found to shift the symmetric and antisymmetric stretching vibrational absorption peaks of phosphodiester group in nucleic acid molecules (P=O). This change suggested that the combined treatment denatured nucleic acid. The combined treatment also changed the protein from an ordered state to a disordered state, and decreased protein stability. The results improved our understanding on the principle of spore inactivation by PATS combining with ε-PL.

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Section: Analysis Of Atpase Activitymentioning

confidence: 99%

Effect of pressure-assisted thermal sterilization combining with ε-polylysine on Bacillus subtilis spore proteins, nucleic acids and other intraspore substances

et al. 2023

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Anti-microbial efficacy and notable biocompatibility of Rosa damascene and Citrus sinensis biomass-derived metabolites

Francis

Namasivayam

Kavisri

et al. 2023

Biomass Conv. Bioref.

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Antagonistic Strain Bacillus velezensis JZ Mediates the Biocontrol of Bacillus altitudinis m-1, a Cause of Leaf Spot Disease in Strawberry

Zhang,

Liu,

et al. 2024

IJMS

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Biofertilizers are environmentally friendly compounds that can enhance plant growth and substitute for chemically synthesized products. In this research, a new strain of the bacterium Bacillus velezensis, designated JZ, was isolated from the roots of strawberry plants and exhibited potent antagonistic properties against Bacillus altitudinis m-1, a pathogen responsible for leaf spot disease in strawberry. The fermentation broth of JZ exerted an inhibition rate of 47.43% against this pathogen. Using an optimized acid precipitation method, crude extracts of lipopeptides from the JZ fermentation broth were obtained. The crude extract of B. velezensis JZ fermentation broth did not significantly disrupt the cell permeability of B. altitudinis m-1, whereas it notably reduced the Ca2+-ATPase activity on the cell membrane and markedly elevated the intracellular reactive oxygen species (ROS) concentration. To identify the active compounds within the crude extract, QTOF-MS/MS was employed, revealing four antimicrobial compounds: fengycin, iturin, surfactin, and a polyene antibiotic known as bacillaene. The strain JZ also produced various plant-growth-promoting substances, such as protease, IAA, and siderophore, which assists plants to survive under pathogen infection. These findings suggest that the JZ strain holds significant potential as a biological control agent against B. altitudinis, providing a promising avenue for the management of plant bacterial disease.

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Antibacterial activity and mechanism of phillyrin against selected four foodborne pathogens

Cited by 3 publications

References 36 publications

Effect of pressure-assisted thermal sterilization combining with ε-polylysine on Bacillus subtilis spore proteins, nucleic acids and other intraspore substances

Effect of pressure-assisted thermal sterilization combining with ε-polylysine on Bacillus subtilis spore proteins, nucleic acids and other intraspore substances

Anti-microbial efficacy and notable biocompatibility of Rosa damascene and Citrus sinensis biomass-derived metabolites

Antagonistic Strain Bacillus velezensis JZ Mediates the Biocontrol of Bacillus altitudinis m-1, a Cause of Leaf Spot Disease in Strawberry

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