Antibacterial Activities of Novel Diselenide-bridged bis(Porphyrin)s on Staphylococcus aureus Investigated by Microcalorimetry

Xu, Xiangjiao; Xue, Zhi; Xiao, Qi; Hou, An Xin; Liu, Yi

doi:10.1007/s12011-008-8156-1

Cited by 21 publications

(13 citation statements)

References 19 publications

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“…Microcalorimetry can be used to study the metabolism of cell and the effect of the toxicants on cellular metabolism [14][15][16][17]. Beer demonstrated microcalorimetric measurements of bacterial growth revealed by temporal details not observed by other techniques [18].…”

Section: Introductionmentioning

confidence: 99%

Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Ding

Liu

et al. 2010

Biol Trace Elem Res

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The biological effect of Se and Cu²(+) on Escherichia coli (E. coli) growth was studied by using a 3114/3236 TAM Air Isothermal Calorimeter, ampoule method, at 37°C. From the thermogenesis curves, the thermokinetic equations were established under different conditions. The kinetics showed that a low concentration of Se (1-10 μg/mL) promoted the growth of E. coli, and a high concentration of Se (>10 μg/mL) inhibited the growth, but the Cu²(+) was always inhibiting the growth of E. coli. Moreover, there was an antagonistic or positive synergistic effect of Se and Cu²(+) on E. coli in the different culture medium when Se was 1-10 μg/ml and Cu²(+) was 1-20 μg/ml. There was a negative synergistic effect of Se and Cu²(+) on E. coli when Se was higher than 10 μg/ml and Cu²(+) was higher than 20 μg/ml. The antagonistic or synergistic effect between Se and Cu²(+) on E. coli was related to the formation of Cu-Se complexes under the different experimental conditions chosen.

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Section: Introductionmentioning

confidence: 99%

Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Ding

Liu

et al. 2010

Biol Trace Elem Res

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“…In the last few years, it has been used as a tool to determine inhibitory effects and/or the minimal inhibitory concentration (MIC) for different antimicrobial compounds [8][9][10][11][12][13] and antibiotics within hours [14][15][16][17][18][19].…”

Section: Microcalorimetry and Its Applicationsmentioning

confidence: 99%

Microcalorimetric method to determine ceftazidime and piperacillin–tazobactam susceptibility in Pseudomonas aeruginosa

López

Rivero

Vázquez

et al. 2015

J Therm Anal Calorim

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Pseudomonas aeruginosa is a gram-negative bacterium, considered one of the most common causes of nosocomial infections. Bacterial cultures produce an exchange of energy as a result of their metabolism. The rate of heat production is an adequate measure of the metabolic activity of the organisms and their constituent parts. Microorganisms produce small amounts of heat, in the order of 1-3 pW per cell. Although the heat produced by bacteria is very small, their exponential reproduction in a culture medium permits heat detection through microcalorimetry.In this work, we analysed the microcalorimetric behaviour of P. aeruginosa and its susceptibility against two antibiotics: ceftazidime and piperacillin-tazobactam. We performed this experiment five times using different concentrations of antibiotics (2 9 MIC, 1 9 MIC, 0.75 9 MIC, 0.5 9 MIC y 0.25 9 MIC). The effect of antibiotics on bacterial growth can be evaluated by the delay of heat flow and reduction of the heat flow peak compared to results with the growth control without the presence of antibiotics. The measures were carried out using a Calvet microcalorimeter that contains two stainless steel cells of approximately 10 cm 3 (reference and experimental). A constant temperature of 309.65 K was maintained within the microcalorimeter. Recording the difference in calorific potential over time, we obtained the growth curves of P. aeruginosa.

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“…In recent years, main drugs clinical used in the treatment of fungal infections are imidazole, carbamates, and polyenes. These drugs present much higher efficacy and lower toxicity compared with previous drugs (Buckton et al, 1991;Xu et al, 2008). Among these drugs, azoles show high efficiency, low recurrence rate, and adverse reactions are light.…”

Section: Introductionmentioning

confidence: 99%

Antifungal efficiency of miconazole and econazole and the interaction with transport protein: A comparative study

Zhang

Cheng

2014

Pharmaceutical Biology

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Context: Miconazole (MIZ) and econazole (ECZ) are clinically used as antifungal drugs. Objective: The drug effect and binding property with transport protein human serum albumin of MIZ and ECZ were studied. Materials and methods: The antifungal efficiency was investigated by microdiluting drug solutions from 0 to 48 mmol L À1 through microcalorimetry and voltammetry studies. Transmission electron microscopy was used for morphological observations of C. albicans. The interaction with HSA was studied by electrochemical methods, fluorescence spectrometry, electron microscopy, and molecular simulation. , the peak currents increased to 4.887 and 6.024 mA. The peak currents of C. albicans in the presence of 20 and 48 mmol L À1 ECZ were 4.701 and 5.544 mA. The docking scores for MIZ and ECZ of the best binding conformation in site I and site II were 5.60, 4.79, 5.63, and 5.85. Discussion and conclusion: Strong inhibition to the metabolism of C. albicans and destructive effect was proved for both drugs. The lower IC 50 , growth rate constant of C. albicans, and higher peak current, reveal stronger antifungal activity of MIZ. Both drugs show an efficient quenching effect to intrinsic fluorescence residues of protein. MIZ mainly binds on site I while ECZ on site II. Molecular modeling experiments give further insight of the binding mechanism.

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Antibacterial Activities of Novel Diselenide-bridged bis(Porphyrin)s on Staphylococcus aureus Investigated by Microcalorimetry

Cited by 21 publications

References 19 publications

Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Microcalorimetric method to determine ceftazidime and piperacillin–tazobactam susceptibility in Pseudomonas aeruginosa

Antifungal efficiency of miconazole and econazole and the interaction with transport protein: A comparative study

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