Antibacterial Activities of Naturally Occurring Compounds against Mycobacterium avium subsp. paratuberculosis

Abstract

The antibacterial activities of 18 naturally occurring compounds (including essential oils and some of their isolated constituents, apple and green tea polyphenols, and other plant extracts) against three strains of Mycobacterium avium subsp. paratuberculosis (a bovine isolate [NCTC 8578], a raw-milk isolate [806R], and a human isolate [ATCC 43015]) were evaluated using a macrobroth susceptibility testing method. M. avium subsp. paratuberculosis was grown in 4 ml Middlebrook 7H9 broth containing 10% oleic acid-albumin-dextrosecatalase, 0.05% Tween 80 (or 0.2% glycerol), and 2 g/ml mycobactin J supplemented with five concentrations of each test compound. The changes in the optical densities of the cultures at 600 nm as a measure of CFU were recorded at intervals over an incubation period of 42 days at 37°C. Six of the compounds were found to inhibit the growth of M. avium subsp. paratuberculosis. The most effective compound was trans-cinnamaldehyde, with a MIC of 25.9 g/ml, followed by cinnamon oil (26.2 g/ml), oregano oil (68.2 g/ml), carvacrol (72.2 g/ml), 2,5-dihydroxybenzaldehyde (74 g/ml), and 2-hydroxy-5-methoxybenzaldehyde (90.4 g/ml). With the exception of carvacrol, a phenolic compound, three of the four most active compounds are aldehydes, suggesting that the structure of the phenolic group or the aldehyde group may be important to the antibacterial activity. No difference in compound activity was observed between the three M. avium subsp. paratuberculosis strains studied. Possible mechanisms of the antimicrobial effects are discussed.

Antimicrobial activity of pomegranate fruit constituents against drug-resistantMycobacterium tuberculosisandβ-lactamase producingKlebsiella pneumoniae

“…paratuberculosis strains (Wong et al, 2008). Actually, the previous investigations regarding antibacterial/antitubercular activity of aforesaid plant-derived polyphenols were mostly limited to target-specific molecular interactions (Biswas et al, 2013;Gradisar et al, 2007;Sharma et al, 2008;Suriyanarayanan et al, 2013;Zhang & Rock, 2004).…”

A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review.

“…paratuberculosis -trans-Cinnamaldehyde MIC=25.9 μg/ml [156] M. avium subsp. paratuberculosis -Carvacrol MIC=72.2 μg/ml [156] M. avium subsp.…”

“…paratuberculosis -trans-Cinnamaldehyde MIC=25.9 μg/ml [156] M. avium subsp. paratuberculosis -Carvacrol MIC=72.2 μg/ml [156] M. avium subsp. paratuberculosis -2,5-Dihydroxybenzaldehyde MIC=74 μg/ml [156] M. avium subsp.…”

“…paratuberculosis -Carvacrol MIC=72.2 μg/ml [156] M. avium subsp. paratuberculosis -2,5-Dihydroxybenzaldehyde MIC=74 μg/ml [156] M. avium subsp. paratuberculosis -2-Hydroxy-5-methoxybenzaldehyde MIC=90.4 μg/ml [156] G. Lang and G. Buchbauer wileyonlinelibrary.com/journal/ffj EO, among others B. subtilis (MIC of 60mg/ml), S. epidermidis and Enterococcus hirae with a MIC result of 100mg/ml.…”

La méthode Bayésienne pour aider à évaluer l’efficacité des thérapies personnalisées

“…Les huiles essentielles d'Origanum compactum et de cannelle sont connues pour leurs propriétésc ontre Mycobacterium avium [40]. L'utilisation de cette huile serait-elle bénéfique sur l'évolution de la maladie [41] ?…”